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Mouse MARCO Antibody

Catalog #: MAB29561
2 Citations Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB29561-100
MAB29561-SP
Detection of MARCO in J774 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (2)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MARCO Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MARCO in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2359A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line, NS0-derived mouse MARCO protein
Gln70-Ser518
Accession # Q60754
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
J774 mouse monocyte-macrophage cell line treated with LPS
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MARCO antibody in J774 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of MARCO in J774 Mouse Cell Line by Flow Cytometry. J774 mouse monocyte-macrophage cell line, resting (open histogram), or treated with 500 ng/ml LPS for 3 days (filled histogram) were stained with Rabbit Anti-Mouse MARCO Monoclonal Antibody (Catalog # MAB29561) followed by anti-Rabbit IgG APC-conjugated secondary antibody (F0111). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MARCO

MARCO (macrophage receptor with collagenous structure), also known as SCARA2, is an 80 kDa type II transmembrane glycoprotein that belongs to the class A scavenger receptor family (1). Mouse MARCO consists of a 48 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 449 aa extracellular domain (ECD) that includes a stalk region, a collagen-like region, and one SRCR domain (2). Within the ECD, mouse MARCO shares 69% and 86% aa sequence identity with human and rat MARCO, respectively. It shares 18%-28% aa sequence identity with other mouse class A scavenger receptors CL-P1, SCARA3, SCARA5, and SR-A1/MSR. MARCO is constitutively expressed on the surface of splenic and lymph node macrophages (2, 3). Its expression is induced on Kupffer cells and alveolar macrophages by microbial infection, chemical irritants, and Th1 polarizing factors (3-5). MARCO binds LPS, lipoteichoic acid, and other determinants on Gram positive and Gram negative bacteria (2, 6-8). It also binds modified LDL, CpG oligonucleotides, UGRP1, silica, and TiO2 (2, 9-11). MARCO is required for the organization of the splenic marginal zone and the interaction of local macrophages and B cells (12, 13). The SRCR domain mediates binding of MARCO to its various ligands (3, 12), while the collagen-like region mediates assembly into a disulfide-linked trimeric molecule (2, 7). MARCO ligation induces, but is not required for the production of IL-12, NO, or TNF-alpha by macrophages (5, 6, 9). MARCO knockout mice show a reduced clearance of bacterial infections, reduced mast cell mediated silicosis, increased pulmonary inflammation, and increased sensitivity to ozone induced lung damage (4, 9, 14-16).

References
  1. Murphy, J.E. et al. (2005) Atherosclerosis 182:1.
  2. Elomaa, O. et al. (1995) Cell 80:603.
  3. Van der Laan, L.J.W. et al. (1999) J. Immunol. 162:939.
  4. Dahl, M. et al. (2007) J. Clin. Invest. 117:757.
  5. Jozefowski, S. et al. (2005) J. Immunol. 175:8032. 
  6. Mukhopadhyay, S. et al. (2006) Eur. J. Immunol. 36:940. 
  7. Sankala, M. et al. (2002) J. Biol. Chem. 277:33378. 
  8. Chen, Y. et al. (2006) J. Biol. Chem. 281:12767. 
  9. Jozefowski, S. et al. (2006) J. Leukoc. Biol. 80:870.
  10. Bin, L.-H. et al. (2003) J. Immunol. 171:924.
  11. Hamilton, Jr. R.F. et al. (2006) J. Biol. Chem. 281:34218.
  12. Karlsson, M.C.I. et al. (2003) J. Exp. Med. 198:333.
  13. Chen, Y. et al. (2005) J. Immunol. 175:8173.
  14. Arredouani, M. et al. (2004) J. Exp. Med. 200:267.
  15. Arredouani, M.S. et al. (2007) J. Immunol. 178:5912.
  16. Brown, J.M. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:43.
Long Name
Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2
Entrez Gene IDs
8685 (Human); 17167 (Mouse)
Alternate Names
macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2; MARCO; member 2; SCARA2

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Citations for Mouse MARCO Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Age-associated disparity in phagocytic clearance affects the efficacy of cancer nanotherapeutics
    Authors: Wang, Y;Deng, W;Lee, D;Yan, L;Lu, Y;Dong, S;Huntoon, K;Antony, A;Li, X;Ye, R;Zhao, Y;Zhao, F;Schrank, BR;Ha, J;Kang, M;Yang, M;Gong, P;Lorenzi, PL;Tan, L;Gallup, TD;Tang, SK;Yang, Z;Li, J;Sanford, NN;Wang, H;Kim, BYS;Jiang, W;
    Nature nanotechnology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. In vivo induction of activin A-producing alveolar macrophages supports the progression of lung cell carcinoma
    Authors: S Taniguchi, T Matsui, K Kimura, S Funaki, Y Miyamoto, Y Uchida, T Sudo, J Kikuta, T Hara, D Motooka, YC Liu, D Okuzaki, E Morii, N Emoto, Y Shintani, M Ishii
    Nature Communications, 2023-01-17;14(1):143.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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