Mouse MyBPC3 Antibody

Catalog #: MAB7199 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7199
MAB7199-SP

Detection of Mouse MyBPC3 by Western Blot.

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Mouse MyBPC3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MyBPC3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human MyBPC3 is observed.

Source

Monoclonal Rat IgG₁ Clone # 751631

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse MyBPC3
Pro2-Ser169
Accession # O70468

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse MyBPC3 by Western Blot. Western blot shows lysates of mouse embryonic heart tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse MyBPC3 Monoclonal Antibody (Catalog # MAB7199) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for MyBPC3 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MyBPC3

MyBPC3 (Myosin-binding protein C-cardiac type) is a 140-150 kDa member of the MyBP family, Ig superfamily of proteins. It is expressed in cardiac muscle, and contributes both to myosin filament structure by interacting with light meromysin, and the regulation of contraction by binding to myosin subfragment-2, which results in a reduction of actomyosin ATPase activity. Mouse MyBPC3 is 1270 amino acids (aa) in length. It contains five consecutive C2-type Ig-like domains (aa 151-767), three FN type III repeats (aa 768-958), and two additional C-terminal Ig-like domains (aa 967-1270). There are at least three utilized phosphorylation sites and one Pro‑rich region (aa 100-150). In human, multiple mutations generate variable-length premature truncated forms of MyBPC3. There are two potential isoform variants. One basically shows a six aa substitution for aa 339-344, while a second contains the same six aa substitution coupled to an eight aa extension at the N-terminus. MyBPC3 is a thick filament-associated protein located in the crossbridge region of cardiac muscle sarcomeres. It is known to be a physiological substrate of cAMP‑dependent protein kinase. MyBPC3 has a role in sarcomeric structure and in the regulation of cardiac muscle contraction. MyBPC3 is released into the coronary effluent during myocardial infarction. Mutations in MYBPC3 are associated with hypertrophic cardiomyopathy. Within aa 2-169, mouse MyBPC3 shares 71% and 85% aa sequence identity with human and rat MyBPC3, respectively.

Long Name

Myosin Binding Protein C, Cardiac

Entrez Gene IDs

4607 (Human); 17868 (Mouse)

Alternate Names

Cardiac MyBP-C; CMH4; C-protein, cardiac muscle isoform; DKFZp779E1762; FHC; MYBP-C; MyBPC3; myosin binding protein C, cardiac; myosin-binding protein C, cardiac; myosin-binding protein C, cardiac-type