Mouse PLZF Antibody

Catalog #: MAB29441 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB29441-100
MAB29441-SP

Detection of PLZF in Mouse Splenocytes by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse PLZF Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse PLZF in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 816421

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse PLZF
Met1-Gln254
Accession # NP_001028496

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of PLZF antibody in Mouse Splenocytes antibody by Flow Cytometry.

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Detection of PLZF in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes stimulated with Recombinant Mouse IL-12 (Catalog # 419-ML) and Recombinant Mouse IL-18 (Catalog # 9139-IL) for 3 days were stained with Rat Anti-Mouse NKp46/NCR1 PE-conjugated Monoclonal Antibody (Catalog # FAB22252P) and either (A) Rat Anti-Mouse PLZF Monoclonal Antibody (Catalog # MAB29441) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLZF

Mouse PLZF, also known as Zinc finger and BTB domain containing protein 16 (ZBTB-16) PLZF and ZNF145, is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid (aa) protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc finger domain. PLZF forms dimers with RAR alpha and LAZ3 within its zinc finger region. Alternate splice forms exist that are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. PLZF is highly expressed in undifferentiated, multi-potential hematopoietic progenitor cells, with levels declining as cells commit to various lineages. Mouse PLZF shares 98% and 96% aa identity with rat and human PLZF, respectively.

Long Name

Promyelocytic Leukemia Zinc Finger

Entrez Gene IDs

7704 (Human); 235320 (Mouse)

Alternate Names

PLZF; PLZFzinc finger and BTB domain-containing protein 16; ZBTB16; zinc finger and BTB domain containing 16; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); Zinc finger protein 145; Zinc finger protein PLZF; ZNF145; ZNF145Promyelocytic leukemia zinc finger protein