Mouse/Rat CD25/IL-2 R alpha PE-conjugated Antibody

Name: Mouse/Rat CD25/IL-2 R alpha PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB6750P

Catalog #: FAB6750P Datasheet / COA / SDS

Discontinued Product

FAB6750P has been discontinued.

View all CD25/IL-2R alpha products.

Detection of CD25/IL-2 R alpha in Mouse Splenocytes by Flow Cytometry.

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All CD25/IL-2R alpha Antibodies

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Mouse/Rat CD25/IL-2 R alpha PE-conjugated Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse CD25/IL-2 R alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human CD25/IL-2 R alpha is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD25/IL-2 R alpha
Glu22-Lys236
Accession # Q544I2

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD25/IL-2 R alpha in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD25/IL-2 Ra PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6750P) and Anti-Mouse CD4 APC-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CD25/IL-2 R alpha in Rat CD4⁺T cells by Flow Cytometry.

Rat CD4⁺T cells purified with the Rat CD4⁺T cell Enrichment Column (Catalog # RCD4C-1000) were stained with Goat Anti-Mouse/Rat CD25/IL-2 Ra PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6750P) and Anti-Rat CD4 APC-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD25/IL-2R alpha

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma _c). IL-2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1-3). The mouse IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signal peptide, a 215 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain (4, 5). Within the ECD, mouse IL-2 R alpha shares 81% and 58% aa sequence identity with rat and human IL-2 R alpha, respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma _c. IL-2 R beta (CD122) and gamma _c (IL-2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (6, 7). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma _c to generate a ternary high affinity IL-2 receptor complex (8). A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (9, 10). Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders (11). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (12, 13). IL-2 R alpha is also required for the development of CD4⁺CD25⁺ Treg which suppress autoreactive CD4⁺ T cells, thereby contributing to peripheral T cell homeostasis (12-14).

References

Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
Kovanen, P.E. and W.J. Leonard (2004) Immunol. Rev. 202:67.
Bluestone, J.A. and Q. Tang (2005) Curr. Opin. Immunol. 17:638.
Miller, J. et al. (1985) J. Immunol. 134:4212.
Shimuzu, A. et al. (1985) Nucleic Acids Res. 13:1505.
Hatakeyama, M. et al. (1989) Science 244:551.
Takeshita, T. et al. (1992) Science 257:379.
Wang, X. et al. (2005) Science 310:1159.
Wagner, D.K. et al. (1986) J. Immunol. 137:592.
Schulz, O. et al. (1998) J. Exp. Med. 187:271.
Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
Willerford, D.M. et al. (1995) Immunity 3:521.
Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.

Long Name

Interleukin 2 Receptor alpha

Entrez Gene IDs

3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)

Alternate Names

CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR