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Mouse/Rat PTP1B Antibody

Catalog #: AF3954
14 Citations Datasheet / COA / SDS
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AF3954
AF3954-SP

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Detection of Mouse/Rat PTP1B by Western Blot.
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Product Details
Citations (14)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat PTP1B Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat PTP1B in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human PTP1B is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse PTP1B
Met1-Phe431
Accession # P35821
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.3 µg/mL
See below
Simple Western
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse/Rat PTP1B antibody by Western Blot. View Larger

Detection of Mouse/Rat PTP1B by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.3 µg/mL Goat Anti-Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3954) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human PTP1B (Catalog # 1366-PT) and recombinant mouse PTP1B 5 ng/lane) were included. A specific band for PTP1B was detected at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Mouse PTP1B antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Mouse PTP1B by Simple WesternTM. Simple Western lane view shows lysates of Neuro-2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTP1B at approximately 56 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3954) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTP1B

Protein tyrosine phosphatase 1B (PTP1B), also known as protein tyrosine phosphatase, non-receptor type 1 (PTPN1) and placental protein tyrosine phosphatase, dephosphorylates tyrosine residues in proteins. PTP1B is a cytosolic enzyme anchored in the endoplasmic reticulum that plays a critical role in regulating growth factor responsiveness. PTP1B knockout mice show increased resistance to high fat diet-induced obesity and have exaggerated responses to insulin.

Long Name
Protein Tyrosine Phosphatase 1B
Entrez Gene IDs
5770 (Human); 19246 (Mouse)
Alternate Names
EC 3.1.3.48; protein tyrosine phosphatase 1B; protein tyrosine phosphatase, non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP1B; PTP-1B; PTP1Bprotein tyrosine phosphatase, placental; PTPN1; tyrosine-protein phosphatase non-receptor type 1

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Citations for Mouse/Rat PTP1B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Comprehensive analysis of PTPN family expression and prognosis in acute myeloid leukemia
    Authors: Yong Liu, Jing Zhang, Zefan Du, Junbin Huang, Yucai Cheng, Wenfang Yi et al.
    Frontiers in Genetics
  2. Protein Tyrosine Phosphatase 1B Deficiency Improves Glucose Homeostasis in Type 1 Diabetes Treated With Leptin
    Authors: Yoshihiro Ito, Runan Sun, Hiroshi Yagimuma, Keigo Taki, Akira Mizoguchi, Tomoko Kobayashi et al.
    Diabetes
  3. PTP1B Is an Intracellular Checkpoint that Limits T-cell and CAR T-cell Antitumor Immunity
    Authors: Florian Wiede, Kun-Hui Lu, Xin Du, Mara N. Zeissig, Rachel Xu, Pei Kee Goh et al.
    Cancer Discovery
  4. The antioxidant role of STAT3 in methylmercury-induced toxicity in mouse hypothalamic neuronal GT1-7 cell line
    Authors: Beatriz Ferrer, Harshini Suresh, Abel Santamaria, João Batista Rocha, Aaron B. Bowman, Michael Aschner
    Free Radical Biology and Medicine
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Chronic exposure to methylmercury enhances the anorexigenic effects of leptin in C57BL/6J male mice
    Authors: Beatriz Ferrer, Lisa M. Prince, Alexey A. Tinkov, Abel Santamaria, Marcelo Farina, João Batista Rocha et al.
    Food and Chemical Toxicology
  6. Hepatic protein-tyrosine phosphatase 1B disruption and pharmacological inhibition attenuate ethanol-induced oxidative stress and ameliorate alcoholic liver disease in mice
    Authors: MF Hsu, S Koike, A Mello, LE Nagy, FG Haj
    Redox Biol, 2020-07-24;36(0):101658.
    Species: Human, Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  7. Intranasal Targeting of Hypothalamic PTP1B and TCPTP Reinstates Leptin and Insulin Sensitivity and Promotes Weight Loss in Obesity
    Authors: GT Dodd, CE Xirouchaki, M Eramo, CA Mitchell, ZB Andrews, BA Henry, MA Cowley, T Tiganis
    Cell Rep, 2019-09-10;28(11):2905-2922.e5.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  8. Adrenalectomy impairs insulin-induced hypophagia and related hypothalamic changes
    Authors: Ernane Torres Uchoa, Paula Beatriz Marangon, Rodrigo Rorato, Silvia Graciela Ruginsk, Lucas Kniess Debarba, Jose Antunes-Rodrigues et al.
    Journal of Endocrinology
  9. Obesity Drives STAT-1-Dependent NASH and STAT-3-Dependent HCC
    Authors: Marcus Grohmann, Florian Wiede, Garron T. Dodd, Esteban N. Gurzov, Geraldine J. Ooi, Tariq Butt et al.
    Cell
  10. Pathological Oxidation of PTPN12 Underlies ABL1 Phosphorylation in Hereditary Leiomyomatosis and Renal Cell Carcinoma
    Authors: Y Xu, P Taylor, J Andrade, B Ueberheide, B Shuch, PM Glazer, RS Bindra, MF Moran, WM Linehan, BG Neel
    Cancer Res., 2018-10-08;0(0):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Assay to visualize specific protein oxidation reveals spatio-temporal regulation of SHP2
    Authors: R Tsutsumi, J Harizanova, R Stockert, K Schröder, PIH Bastiaens, BG Neel
    Nat Commun, 2017-09-06;8(1):466.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  12. Deficiency of PTP1B Attenuates Hypothalamic Inflammation via Activation of the JAK2-STAT3 Pathway in Microglia
    Authors: T Tsunekawa, R Banno, A Mizoguchi, M Sugiyama, T Tominaga, T Onoue, D Hagiwara, Y Ito, S Iwama, M Goto, H Suga, Y Sugimura, H Arima
    EBioMedicine, 2017-01-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. Ventromedial hypothalamus–specific Ptpn1 deletion exacerbates diet-induced obesity in female mice
    Authors: Franck Chiappini, Karyn J. Catalano, Jennifer Lee, Odile D. Peroni, Jacqueline Lynch, Abha S. Dhaneshwar et al.
    Journal of Clinical Investigation
  14. Targeting density-enhanced phosphatase-1 (DEP-1) with antisense oligonucleotides improves the metabolic phenotype in high-fat diet-fed mice
    Authors: Janine Krüger, Manuela Trappiel, Markus Dagnell, Philipp Stawowy, Heike Meyborg, Christian Böhm et al.
    Cell Communication and Signaling

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