Mouse STAT6 Antibody

Name: Mouse STAT6 Antibody
Brand: R&D Systems
SKU: MAB2169
Rating: 5 (1 reviews)

Catalog #: MAB2169 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2169
MAB2169-SP

Detection of Mouse STAT6 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse STAT6 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects human, mouse, and rat STAT6 in Western blots.

Source

Monoclonal Rat IgG_2A Clone # 69727

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse STAT6
Ser627-Trp837
Accession # P52633

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse STAT6 by Western Blot. Western blot shows lysates of DA3 mouse myeloma cell line untreated (-) or treated (+) with 25 µg Recombinant Mouse IL-4 (Catalog # 404-ML) for 30 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse STAT6 Monoclonal Antibody (Catalog # MAB2169), followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for STAT6 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT6

STAT family proteins mediate cytokine signaling by acting as signal transducers in the cytoplasm and transcription activators in the nucleus. STAT6 is important for the development of Th2 cells and regulation of gene expression by IL-4 and IL-13.

Long Name

Signal Transducer and Activator of Transcription 6

Entrez Gene IDs

6778 (Human); 20852 (Mouse); 362896 (Rat)

Alternate Names

D12S1644; EC 2.4.1.227; EC 2.7.7.6; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin-4 induced; STAT, interleukin4-induced; STAT6; STAT6B; STAT6C; transcription factor IL-4 STAT