Mouse TNFRH3/TNFRSF26 Antibody

Catalog #: MAB5330 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5330
MAB5330-SP

Detection of TNFRH3/TNFRSF26 in Mouse Splenocytes by Flow Cytometry.

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Mouse TNFRH3/TNFRSF26 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TNFRH3/TNFRSF26 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Fas, rmTNF RI, and rmTNF RII is observed.

Source

Monoclonal Rat IgG_2A Clone # 509027

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse TNFRH3/TNFRSF26
Ser20-Lys162
Accession # P83626

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TNFRH3/TNFRSF26 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse TNFRH3/TNFRSF26 Monoclonal Antibody (Catalog # MAB5330, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNFRH3/TNFRSF26

Tumor necrosis factor receptor homolog 3 (TNFRH3; also tumor necrosis factor receptor superfamily member 26) is a 21 kDa (predicted, unglycosylated) type I transmembrane protein and member of the TNF receptor superfamily. The ECD contains two potential sites of N-linked glycosylation and a cysteine-rich region (aa 38‑126). There is no human ortholog for TNFRH3. TNFRH3 is expressed in thymus and spleen with detectable levels in the lung.

Long Name

Tumor Necrosis Factor Receptor Homolog 3

Entrez Gene IDs

244237 (Mouse)

Alternate Names

APO-3;DDR3;DR3;GEF720;LARD;PLEKHG5;TNFRSF12;TNFRSF25;TR3;TRAMP;Tumor necrosis factor receptor superfamily member 25;WSL-1;WSL-LR; TNFRH3; Tnfrsf24; Tnfrsf26