Mouse VSIG2 Antibody

Catalog #: MAB4766 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4766
MAB4766-SP

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Mouse VSIG2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse VSIG2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human VSIG2 or recombinant mouse VSIG1 is observed.

Source

Monoclonal Rat IgG₁ Clone # 771625

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VSIG2
Val25-Ala244 (Glu240Asp)
Accession # Q9Z109

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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VSIG2 in Mouse Stomach. VSIG2 was detected in perfusion fixed frozen sections of mouse stomach using Rat Anti-Mouse VSIG2 Monoclonal Antibody (Catalog # MAB4766) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to the plasma membranes of cells in gastric glands. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG2

VSIG2 (V-set and Ig domain-containing protein 2; also CTM and CT-like protein) is presumably a 50‑60 kDa member of the CTX family of Ig-Superfamily proteins. It shows expression in stomach and prostate by Northern blot, and likely participates in cell adhesion. Mouse VSIG2 precursor is 328 amino acids (aa) in length. It is a type I transmembrane (glyco)protein that contains a 220 aa extracellular domain (ECD) (aa 25‑244) and a 63 aa cytoplasmic region. The ECD contains one V-type (aa 25‑138) and one C2-type Ig-like domain (aa 145‑234). Over aa 25‑244, mouse VSIG2 is 94%, 83% and 85% aa identical to rat, canine and human VSIG2, respectively. Two potential splice variants exist, one that shows a deletion of aa 305‑328 and a second that shows a deletion of aa 144‑236.

Long Name

V-Set and Immunoglobulin Domain Containing 2

Entrez Gene IDs

23584 (Human); 57276 (Mouse); 300520 (Rat)

Alternate Names

2210413P10Rik; Cortical thymocyte-like protein; CTH; CTHCTXLcortical thymocyte receptor (X. laevis CTX) like; CT-like protein; CTXL; V-set and immunoglobulin domain containing 2; V-set and immunoglobulin domain-containing protein 2; VSIG2