Normal Goat IgG Biotinylated Control

Catalog # Availability Size / Price Qty
BAF108
R&D Systems Antibodies
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Product Details
Citations (35)
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Supplemental Products
Reviews (4)

Normal Goat IgG Biotinylated Control Summary

Specificity
Serum was obtained from naive (non-immunized) goats.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
NA
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Normal Goat IgG Biotinylated Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. RNA sequencing of pancreatic circulating tumour cells implicates WNT signalling in metastasis.
    Authors: Yu M, Ting DT, Stott SL et al.
    Nature
  2. Elevated apolipoprotein C3 augments diabetic kidney disease and associated atherosclerosis in type 2 diabetes
    Authors: Cervantes, J;Koska, J;Kramer, F;Akilesh, S;Alpers, CE;Mullick, AE;Reaven, P;Kanter, JE;
    JCI insight
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. Transgelin 2 guards T cell lipid metabolic programming and anti-tumor function
    Authors: Hwang, SM;Awasthi, D;Jeong, J;Sandoval, TA;Chae, CS;Ramos, Y;Tan, C;Falco, MM;McBain, IT;Mishra, B;Ivashkiv, LB;Zamarin, D;Cantillo, E;Chapman-Davis, E;Holcomb, K;Morales, DK;Rodriguez, PC;Conejo-Garcia, JR;Kaczocha, M;Vähärautio, A;Song, M;Cubillos-Ruiz, JR;
    Research square
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  4. Tumor-derived interleukin-1? and leukemia inhibitory factor promote extramedullary hematopoiesis
    Authors: Barisas, DAG;Kabir, AU;Wu, J;Krchma, K;Kim, M;Subramanian, M;Zinselmeyer, BH;Stewart, CL;Choi, K;
    PLoS biology
  5. Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis
    Authors: S Xu, X Yang, Q Chen, Z Liu, Y Chen, X Yao, A Xiao, J Tian, L Xie, M Zhou, Z Hu, F Zhu, X Xu, F Hou, J Nie
    EBioMedicine, 2022-11-04;86(0):104312.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. The immune checkpoint B7-H3 (CD276) regulates adipocyte progenitor metabolism and obesity development
    Authors: E Picarda, PM Galbo, H Zong, MR Rajan, V Wallenius, D Zheng, E Börgeson, R Singh, J Pessin, X Zang
    Science Advances, 2022-04-27;8(17):eabm7012.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  7. The Influence of an Elastase-Sensitive Complement C5 Variant on Lupus Nephritis and Its Flare
    Authors: CR Toy, H Song, HN Nagaraja, J Scott, J Greco, X Zhang, CY Yu, JA Tumlin, BH Rovin, LA Hebert, DJ Birmingham
    Kidney international reports, 2021-06-09;6(8):2105-2113.
    Species: Human
    Sample Types: Urine
    Applications: Western Blot Control
  8. Apolipoprotein C3 and apolipoprotein B colocalize in proximity to macrophages in atherosclerotic lesions in diabetes
    Authors: Jenny E. Kanter, Karin E. Bornfeldt
    Journal of Lipid Research
  9. Sequence‐Defined Heteromultivalent Precision Glycomacromolecules Bearing Sulfonated/Sulfated Nonglycosidic Moieties Preferentially Bind Galectin‐3 and Delay Wound Healing of a Galectin‐3 Positive Tumor Cell Line in an In Vitro Wound Scratch Assay
    Authors: Tanja Freichel, Viktoria Heine, Dominic Laaf, Eleanor E. Mackintosh, Sophia Sarafova, Lothar Elling et al.
    Macromolecular Bioscience
  10. Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice
    Authors: A Carestia, HA Mena, CM Olexen, JM Ortiz Wilc, S Negrotto, AE Errasti, RM Gómez, CN Jenne, EA Carrera Si, M Schattner
    Cell Rep, 2019-07-23;28(4):896-908.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Induction of MET Receptor Tyrosine Kinase Down-regulation through Antibody-mediated Receptor Clustering
    Authors: W Li, A Dick, F Lu, H Zhang, H Sun
    Sci Rep, 2019-02-13;9(1):1988.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. BMP-7 induces apoptosis in human germinal center B cells and is influenced by TGF-? receptor type I ALK5
    Authors: LK Bollum, K Huse, MP Oksvold, B Bai, VI Hilden, L Forfang, SO Yoon, S Wälchli, EB Smeland, JH Myklebust
    PLoS ONE, 2017-05-10;12(5):e0177188.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. A Core Regulatory Circuit in Glioblastoma Stem Cells Links MAPK Activation to a Transcriptional Program of Neural Stem Cell Identity
    Authors: G Riddick, S Kotliarova, V Rodriguez, HS Kim, A Linkous, AJ Storaska, S Ahn, J Walling, G Belova, HA Fine
    Sci Rep, 2017-03-03;7(0):43605.
    Species: Human
    Sample Types: Chromatin
    Applications: Immunoprecipitation
  14. Expression and function of IL-1R8 (TIR8/SIGIRR), a regulatory member of the IL-1 receptor family in platelets
    Authors: Achille Anselmo
    Cardiovasc Res, 2016-06-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  15. Immunohistochemical Analysis of PD-L1 Expression in Canine Malignant Cancers and PD-1 Expression on Lymphocytes in Canine Oral Melanoma
    Authors: Naoya Maekawa
    PLoS ONE, 2016-06-08;11(6):e0157176.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Interleukin-25 initiates Th2 differentiation of human CD4(+) T cells and influences expression of its own receptor
    Authors: Graeme Bredo, Jessica Storie, Nami Shrestha Palikhe, Courtney Davidson, Alexis Adams, Harissios Vliagoftis et al.
    Immunity, Inflammation and Disease
  17. Deep imaging of bone marrow shows non-dividing stem cells are mainly perisinusoidal
    Authors: Melih Acar, Kiranmai S. Kocherlakota, Malea M. Murphy, James G. Peyer, Hideyuki Oguro, Christopher N. Inra et al.
    Nature
  18. Human group3 innate lymphoid cells express DR3 and respond to TL1A with enhanced IL-22 production and IL-2-dependent proliferation.
    Authors: Ahn Y, Weeres M, Neulen M, Choi J, Kang S, Heo D, Bergerson R, Blazar B, Miller J, Verneris M
    Eur J Immunol, 2015-06-22;45(8):2335-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. MAPK7 Regulates EMT Features and Modulates the Generation of CTCs
    Authors: Sarah Javaid, Jianmin Zhang, Gromoslaw A. Smolen, Min Yu, Ben S. Wittner, Anurag Singh et al.
    Molecular Cancer Research
  20. TACI mutations and impaired B-cell function in subjects with CVID and healthy heterozygotes.
    Authors: Martinez-Gallo M, Radigan L, Almejun M, Martinez-Pomar N, Matamoros N, Cunningham-Rundles C
    J Allergy Clin Immunol, 2012-12-11;131(2):468-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  21. Fusion Toxin BLyS-Gelonin Inhibits Growth of Malignant Human B Cell Lines In Vitro and In Vivo.
    Authors: Luster, Troy A, Mukherjee, Ipsita, Carrell, Jeffrey, Cho, Yun Hee, Gill, Jeffrey, Kelly, Lizbeth, Garcia, Andy, Ward, Christop, Oh, Luke, Ullrich, Stephen, Migone, Thi-Sau, Humphreys, Robin
    PLoS ONE, 2012-10-09;7(10):e47361.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  22. Overexpression of IL-1 receptor accessory protein in stem and progenitor cells and outcome correlation in AML and MDS.
    Authors: Barreyro L, Will B, Bartholdy B, Zhou L, Todorova TI, Stanley RF, Ben-Neriah S, Montagna C, Parekh S, Pellagatti A, Boultwood J, Paietta E, Ketterling RP, Cripe L, Fernandez HF, Greenberg PL, Tallman MS, Steidl C, Mitsiades CS, Verma A, Steidl U
    Blood, 2012-06-21;120(6):1290-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  23. Membrane protein GARP is a receptor for latent TGF-beta on the surface of activated human Treg.
    Authors: Stockis J, Colau D, Coulie PG, Lucas S
    Eur. J. Immunol., 2009-12-01;39(12):3315-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  24. Leptin receptor is expressed in thymus medulla and leptin protects against thymic remodeling during endotoxemia-induced thymus involution.
    Authors: Gruver AL, Ventevogel MS, Sempowski GD
    J. Endocrinol., 2009-07-08;203(1):75-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  25. Interleukin-15 combined with an anti-CD40 antibody provides enhanced therapeutic efficacy for murine models of colon cancer.
    Authors: Zhang M, Yao Z, Dubois S, Ju W, Muller JR, Waldmann TA
    Proc. Natl. Acad. Sci. U.S.A., 2009-04-21;106(18):7513-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Vascular endothelial growth factor-induced chemotaxis and IL-10 from T cells.
    Authors: Shin JY, Yoon IH, Kim JS, Kim B, Park CG
    Cell. Immunol., 2009-02-26;256(1):72-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  27. Insertional gene activation by lentiviral and gammaretroviral vectors.
    Authors: Bokhoven M, Stephen SL, Knight S, Gevers EF, Robinson IC, Takeuchi Y, Collins MK
    J. Virol., 2008-10-22;83(1):283-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  28. Tumor-infiltrating myeloid-derived suppressor cells are pleiotropic-inflamed monocytes/macrophages that bear M1- and M2-type characteristics.
    Authors: Umemura N, Saio M, Suwa T, Kitoh Y, Bai J, Nonaka K, Ouyang GF, Okada M, Balazs M, Adany R, Shibata T, Takami T
    J. Leukoc. Biol., 2008-02-19;83(5):1136-44.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  29. IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells.
    Authors: Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, Kuchen S, Lipsky PE
    J. Immunol., 2007-03-01;178(5):2872-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  30. Regulation of MDK expression in human cancer cells modulates sensitivities to various anticancer drugs: MDK overexpression confers to a multi-drug resistance.
    Authors: Kang HC, Kim IJ, Park HW, Jang SG, Ahn SA, Yoon SN, Chang HJ, Yoo BC, Park JG
    Cancer Lett., 2006-04-27;247(1):40-7.
  31. Resveratrol inhibits myeloma cell growth, prevents osteoclast formation, and promotes osteoblast differentiation.
    Authors: Boissy P, Andersen TL, Abdallah BM, Kassem M, Plesner T, Delaisse JM
    Cancer Res., 2005-11-01;65(21):9943-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Targeting tumor angiogenesis with adenovirus-delivered anti-Tie-2 intrabody.
    Authors: Popkov M, Jendreyko N, McGavern DB, Rader C, Barbas CF
    Cancer Res., 2005-02-01;65(3):972-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  33. Ig-reactive CD4+CD25+ T cells from tolerized (New Zealand Black x New Zealand White)F1 mice suppress in vitro production of antibodies to DNA.
    Authors: La Cava A, Ebling FM, Hahn BH
    J. Immunol., 2004-09-01;173(5):3542-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  34. Enhanced B cell expansion, survival, and humoral responses by targeting death receptor 6.
    Authors: Schmidt CS, Liu J, Zhang T, Song HY, Sandusky G, Mintze K, Benschop RJ, Glasebrook A, Yang DD, Na S
    J. Exp. Med., 2003-01-06;197(1):51-62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  35. Membrane-bound Interleukin-1 alpha mediates leukocyte adhesion during atherogenesis

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Reviews for Normal Goat IgG Biotinylated Control

Average Rating: 4.5 (Based on 4 Reviews)

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Normal Goat IgG Biotinylated Control
By Anonymous on 08/24/2017
Application: Flow Sample Tested: Adult splenocytes Species: Mouse

Used as isotype control


Normal Goat IgG Biotinylated Control
By Anonymous on 06/17/2017
Application: Flow Sample Tested: Peritoneal macrophages Species: Mouse

Normal Goat IgG Biotinylated Control
By Adam Guess on 11/08/2016

Normal Goat IgG Biotinylated Control
By Ramya Narasimhan on 05/02/2016
Application: Affinity Purification Sample Tested: Mouse splenocytes,Murine mesenteric lymph node Species: Mouse