NPE-caged-HPTS
Chemical Name: 8-Hydroxypyrene-1,3,6-tris-sulfonic acid-8-1-(2-nitrophenyl)ethyl ether
Purity: ≥97%
Biological Activity
NPE-caged-HPTS is a caged fluorescent pH indicator. Rapidly releases the fluorophore HPTS (pyranine) (pKa 7.25) upon two-photon excitation (>3000 s-1). Routinely used for photolysis calibration. Suitable for use in small-compartment diffusion studies.Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Photochemical and pharmacological evaluation of 7-nitroindolinyl-and 4-methoxy-7-nitroindolinyl-amino acids as novel, fast caged neurotransmitters.
Canepari et al.
J.Neurosci.Methods., 2001;112:29 -
Synapse-specific plasticity and compartmentalized signaling in cerebellar stellate cells.
Soler-Llavina et al.
Nat.Neurosci., 2006;9:798 -
Comparisons of simultaneous pH measurements made with 8-hydroxypyrene-1,3,6-trisulphonic acid (HPTS) and pH-sensitive microelectrodes in snail neurones.
Willoughby et al.
Pflugers Arch., 1998;436:615 -
Chemotactic responses of Escherichia coli to small jumps of photoreleased L-aspartate.
Jasuja et al.
Biophys.J., 1999;76:1706 -
The efficiency of two-photon photolysis of a "caged " fluorophore, o-1-(2-nitrophenyl)ethylpyranine, in relation to photodamage of synaptic terminals.
Kiskin et al.
Eur.Biophys.J., 2002;30:588 -
Neuronal activity regulates diffusion across the neck of dendritic spines.
Bloodgood and Sabatini
Science, 2005;310:866 -
Laser photolysis of caged compounds at 405 nm: photochemical advantages, localisation, phototoxicity and methods for calibration.
Trigo et al.
J.Neurosci.Methods, 2009;180:9
Product Datasheets
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