Rat CCR6 Antibody

Catalog #: MAB8320
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8320
MAB8320-SP

Detection of CCR6 in HEK293 Human Cell Line Transfected with Rat CCR6 and eGFP by Flow Cytometry.

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All CCR6 Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat CCR6 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CCR6 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 876515

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NSO mouse myeloma cell line transfected with rat CCR6
Met1-Met366
Accession # Q5BK58

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR6 in HEK293 Human Cell Line Transfected with Rat CCR6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with rat CCR6 and eGFP was stained with Mouse Anti-Rat CCR6 Monoclonal Antibody (Catalog # MAB8320) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR6

CCR6 is an approximately 45 kDa G-protein linked seven transmembrane domain receptor that binds the chemokine CCL20/MIP-3 alpha. Among hematopoietic cells, CCR6 is expressed on B cells, immature dendritic cells, neutrophils, and various T cell subsets (e.g. CD4⁺ T cells, regulatory T cells, effector memory Th17 cells, and g/d T cells). It is upregulated in several cancers, Alzheimer’s brain, and the airways in obstructive pulmonary disease. CCR6 plays an important role in mucosal immunity, IgE mediated allergic reactions, immune cell infiltration into sites of inflammation, and tumor cell invasiveness. It also promotes chemotaxis and tumor angiogenesis in response to beta defensins. Rat CCR6 shares 74% and 87% amino acid sequence identity with human and mouse CCR6, respectively.

Entrez Gene IDs

1235 (Human); 12458 (Mouse); 308163 (Rat)

Alternate Names

BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKRL3; CKR-L3; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPRCY4; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Rat CCR6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Desensitization and treatment with APRIL/BLyS blockade in rodent kidney transplant model
Authors: NM Bath, X Ding, NA Wilson, BM Verhoven, BA Boldt, A Sukhwal, SR Reese, SE Panzer, A Djamali, RR Redfield
PLoS ONE, 2019-02-08;14(2):e0211865.
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry
GM-CSF-Producing Th Cells in Rats Sensitive and Resistant to Experimental Autoimmune Encephalomyelitis.
Authors: Zorica Stoji?-Vuk, Ivan Pilipovi?, Ivana Vujnovi?, Mirjana Nacka-Ale, Raisa Petrovi?, Nevena Arsenovi?, Mirjana Dimitrije, Gordana Leposavi?
PLoS ONE, 2016-11-10;0(0):1932-6203.
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry