Key Product Details

Species Reactivity

Human

Applications

Western Blot, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # RB42

Format

BSA Free
View all RBP4/Retinol-Binding Protein 4 Primary Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Human recombinant RBP4.

Specificity

Human plasma RBP4. All MAbs recognize free RBP4 and RBP4 in complex with transthyretin. All MAbs react with apo- as well as holo-RBP4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for RBP4/Retinol-Binding Protein 4 Antibody (RB42) - BSA Free

Western Blot: RBP4/Retinol-Binding Protein 4 Antibody (RB42) [NBP1-05426]

Western Blot: RBP4/Retinol-Binding Protein 4 Antibody (RB42) [NBP1-05426]

Western Blot: RBP4/Retinol-Binding Protein 4 Antibody (RB42) [NBP1-05426] - Immunodetection of RBP4 in Western blotting after SDSelectrophoresis in reducing conditions by MAb RB42 (Cat. NBP1-05426) (lane 1), RB45 (Cat. NBP1-105431) (lane 2), RB48 (Cat. NBP1-105430) (lane 3) and RB51 (Cat.NBP1-105428) (lane 4).
Sandwich ELISA: RBP4/Retinol-Binding Protein 4 Antibody (RB42) [NBP1-05426] - Immunodetection of purified endogenous RBP4 in sandwich immunoassay by RB48-RB42 MAb assay in presence of 5 mM EDTA (blue line) or in absence of EDTA (pink line). Capture antibody: RB48 (Cat NBP1-105430) (1 ug/well) Detection antibody: RB42 (Cat NBP1-05426) labeled with stable Eu3+ chelate (0.2ug/well) Antigen: Native isolated RBP4
Sandwich ELISA: RBP4/Retinol-Binding Protein 4 Antibody (RB42) [NBP1-05426] - Immunoreactivity of native free and native complexed RBP4 after several freeze-thaw cycles measured with the assay RB48 - RB42. Capture antibody: RB48 (Cat. NBP1-105430) (1 ug/well) Detection antibody: RB42 (Cat NBP1-05426) labeled with stable Eu3+ chelate (0.2ug/well) Antigen: Native isolated RBP4.

Applications for RBP4/Retinol-Binding Protein 4 Antibody (RB42) - BSA Free

Application
Recommended Usage

Sandwich ELISA

0.2 ug/well

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RBP4/Retinol-Binding Protein 4

RBP4 is a specific carrier for retinol in the blood. It is a member of the lipocalin protein family. Under physiological conditions, RBP binds transthyretin (TTR) to prevent glomerular filtration of low molecular weight RBP in the kidneys. The RBP/TTR molar ration provides an indirect way to indicate marginal vitamin A deficiency. RBP4 may also play a role in insulin resistance in diabetes.

Alternate Names

RetinolBinding Protein 4

Entrez Gene IDs

5950 (Human)

Gene Symbol

RBP4

Additional RBP4/Retinol-Binding Protein 4 Products

Product Documents for RBP4/Retinol-Binding Protein 4 Antibody (RB42) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RBP4/Retinol-Binding Protein 4 Antibody (RB42) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Adipokines & Insulin Signaling Pathways Adipokines & Insulin Signaling Pathway Thumbnail