Recombinant Canine IL-12 Protein

Catalog #: 2118-CL Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
2118-CL-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
2118-CL-025

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Canine IL-12 Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED₅₀ for this effect is < 2.00 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived canine IL-12 protein

Canine IL-12 p40 (Ile23-Ser329) Accession # Q28268	10-His tag
Canine IL-12 p35 (Arg26-Ser222) (Asp63Glu, Tyr64Leu, Ile65Tyr, Pro66Ser) Accession # Q28267	10-His tag
N-terminus	C-terminus

Accession #

Q28268 (p40) & Q28267 (p35)

N-terminal Sequence
Analysis

Ile23 (p40 subunit) & Arg26 (p35 subunit)

Structure / Form

Disulfide-linked heterodimer

Predicted Molecular Mass

36.3 kDa (p40), 23.6 kDa (p35)

SDS-PAGE

42 kDa and 28 kDa, reducing conditions

Product Datasheets

2118-CL (with carrier)

Product Datasheet

COA

SDS

2118-CL/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2118-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2118-CL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-12

Interleukin 12 (IL-12) is the founding member of the IL-12 family of heterodimeric cytokines, which have important immunological functions. IL-12 is composed of two disulfide-linked subunits of 35 kDa and 40 kDa, respectively. The 35 kDa subunit (p35) is an alpha -helical protein homologous to IL-6 and G-CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also utilized in IL-23. Mature canine p35 is a 197 amino acids (aa) protein that is secreted as a heterodimer linked to p40. It contains two potential N-linked glycosylation sites and shares 91%, 86%, 85%, 56%, and 55% aa sequence identity with feline, human, porcine, rat and mouse p35, respectively. Mature p40 contains 307 aa and can exist in multiple forms, including monomer, homodimer, heterodimer linked to p19 (forming IL-23), and heterodimer linked to p35 (forming IL-12). It is upregulated by substances such as LPS and CpG that activate antigen-presenting cells. Mature canine p40 shows 94%, 85%, 84%, 65%, and 65% aa sequence identity to feline, human, porcine, rat and mouse p40, respectively. Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. The activities of IL-12 are mediated by the receptor complex composed of two type I transmembrane proteins: a 100 kDa ligand-binding subunit (IL-12 R beta 1) and a 130 kDa signal transducing subunit (IL-12 R beta 2). IL-12 facilitates hematopoietic stem cell proliferation, induces NK cell proliferation, promotes IgG production (in mice), and potentiates the expansion and late activation of Th1 CD4+ T cells (1 - 5).

References

Buttner, M. et al. (1997) Cytokine 10:241.
Park, A.Y. and P. Scott (2001) Scand. J. Immunol. 53:529.
Trinchieri, G. et al. (2003) Immunity 19:641.
Brombacher, F. et al. (2003) Trends Immunol. 24:207.
Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.

Long Name

Interleukin 12

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat); 397053 (Porcine); 403977 (Canine); 102115316 (Cynomolgus Monkey); 493741 (Feline); 703205 (Rhesus Macaque)

Alternate Names

CLMF p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35