Recombinant Human IFNA16 Protein, CF

R&D Systems | Catalog # 11156-IF

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human IFNA16 Protein (11156-IF)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P05015.1

Applications

Bioactivity
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Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IFNA16 protein
Cys24-Asp189

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys24

Predicted Molecular Mass

19 kDa

SDS-PAGE

18-22 kDa, under reducing conditions.

Activity

Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129.
The ED50 for this effect is 2.00-50.0 pg/mL.

Scientific Data Images for Recombinant Human IFNA16 Protein, CF

Recombinant Human IFNA16 Protein Bioactivity.

Recombinant Human IFNA16 Protein (Catalog # 11156-IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 2.00-50.0 pg/mL.

Recombinant Human IFNA16 Protein SDS-PAGE.

2 μg/lane of Recombinant Human IFNA16 Protein (Catalog # 11156-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-22 kDa under reducing conditions.

Formulation, Preparation, and Storage

11156-IF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFNA16

Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). Human IFNA2 was originally cloned in the early ‘80s and now more than a dozen closely related IFN alpha subtypes have been identified in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2-4). Structurally, type I IFNs belong to the class of five helical-bundle cytokines, with the IFNA subtypes containing 2 conserved disulfide bonds (5). Mature human IFNA16 shares 60% aa sequence identity with mouse IFNA16. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (6, 7). Individual IFNA subtypes are known to display unique efficacies to viral protection, and IFNA16 has been shown to be an intermediate inducer of IFN-stimulated genes and anti-viral protection (8). IFNA16 has been shown to be upregulated during chronic HIV infection and might play a role in lipid accumulation and fatty acid deposition in porcine muscle (9, 10).

References

  1. Pestka S. et al. (1987) Annu Rev Biochem. 56:727
  2. Goeddel, D.V. et al. (1980) Nature 287:411.
  3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  4. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
  5. Wittling, M.C. et al. (2021) Front Immunol. 11:605673.
  6. van Pesch, V. et al. (2004) J. Virol. 78:8219.
  7. James C.M. et al. (2007) Vaccine. 25(10):1856.
  8. Moll, H.P. et al. (2011) Cytokine. 53:52.
  9. Lavender, K.J. et al. (2016) J. Virol. 90:6001.
  10. Mekchay, S. et al. (2022) Biology. 11:109.

Long Name

Interferon alpha 16

Alternate Names

BC114392, Gm13280, IFN-alpha 16, IFN-alpha WA, IFN-alpha-WA, Ifna6T, IFNalpha WA, Ifnat6, Interferon Alpha-WA

Entrez Gene IDs

3449 (Human); 230398 (Mouse)

Gene Symbol

IFNA16

UniProt

P05015.1

Additional IFNA16 Products

Product Documents for Recombinant Human IFNA16 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human IFNA16 Protein, CF

For research use only

Related Research Areas

Interferons

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