Home / IL-34 / Recombinant Human IL-34 Protein

Recombinant Human IL-34 Protein

Catalog #: 5265-IL
21 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
5265-IL-010/CF

With Carrier

Catalog # Availability Size / Price Qty
5265-IL-010
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Graph showing dose-dependent bioactivity of Human IL-34 protein
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Product Details
Citations (21)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-34 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using human peripheral blood monocytes. Lin, H. et al. (2008) Science 320:807. The ED50 for this effect is 0.75-3.75 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-34 protein
Asn21-Pro242, with a C-terminal 6-His tag
Accession #
NP_689669
N-terminal Sequence
Analysis
Asn21
Predicted Molecular Mass
26.1 kDa
SDS-PAGE
35-38 kDa, reducing conditions

Product Datasheets

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5265-IL (with carrier)

Product Datasheet
COA
SDS

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5265-IL/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5265-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5265-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Graph showing dose-dependent bioactivity of Human IL-34 protein View Larger

Recombinant human IL-34 (5265-IL) induces proliferation in human peripheral blood monocytes. The ED50 for this effect is 0.75-3.75 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-34

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers (1). It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N‑linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level (1). IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R) (1). IL-34 stimulates monocyte proliferation (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit‑macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1).

References
  1. Lin, H. et al. (2008) Science 320:807.
Long Name
Interleukin 34
Entrez Gene IDs
146433 (Human); 76527 (Mouse)
Alternate Names
C16orf77; IL34; IL-34; interleukin 34

Citations for Recombinant Human IL-34 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Reduced SH3RF3 may protect against Alzheimer's disease by lowering microglial pro-inflammatory responses via modulation of JNK and NFkB signaling
    Authors: Patel, R;Cardona, CL;Angeles, E;Singh, G;Ashok, A;Teich, AF;Sproul, AA;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Parkinson's disease-derived ?-synuclein assemblies combined with chronic-type inflammatory cues promote a neurotoxic microglial phenotype
    Authors: Yildirim-Balatan, C;Fenyi, A;Besnault, P;Gomez, L;Sepulveda-Diaz, JE;Michel, PP;Melki, R;Hunot, S;
    Journal of neuroinflammation
    Species: Mouse
    Sample Types: Whole Blood
    Applications: Bioassay
  3. Progranulin haploinsufficiency mediates cytoplasmic TDP-43 aggregation with lysosomal abnormalities in human microglia
    Authors: Sung, W;Noh, MY;Nahm, M;Kim, YS;Ki, CS;Kim, YE;Kim, HJ;Kim, SH;
    Journal of neuroinflammation
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. slan+ monocytes kill cancer cells coated in therapeutic antibody by trogoptosis
    Authors: Finotti, G;Pietronigro, E;Balanzin, C;Lonardi, S;Constantin, G;Chao, MP;Tecchio, C;Vermi, W;Cassatella, MA;
    Cancer immunology research
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Angiogenic and inflammatory responses in human induced microglia-like (iMG) cells from patients with Moyamoya disease
    Authors: Shirozu, N;Ohgidani, M;Hata, N;Tanaka, S;Inamine, S;Sagata, N;Kimura, T;Inoue, I;Arimura, K;Nakamizo, A;Nishimura, A;Maehara, N;Takagishi, S;Iwaki, K;Nakao, T;Masuda, K;Sakai, Y;Mizoguchi, M;Yoshimoto, K;Kato, TA;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Dual-specificity protein phosphatase 6 (DUSP6) overexpression reduces amyloid load and improves memory deficits in male 5xFAD mice
    Authors: Pan, AL;Audrain, M;Sakakibara, E;Joshi, R;Zhu, X;Wang, Q;Wang, M;Beckmann, ND;Schadt, EE;Gandy, S;Zhang, B;Ehrlich, ME;Salton, SR;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Embryoid Bodies
    Applications: Bioassay
  7. Mitochondria dysregulation contributes to secondary neurodegeneration progression post-contusion injury in human 3D in vitro triculture brain tissue model
    Authors: Liaudanskaya, V;Fiore, NJ;Zhang, Y;Milton, Y;Kelly, MF;Coe, M;Barreiro, A;Rose, VK;Shapiro, MR;Mullis, AS;Shevzov-Zebrun, A;Blurton-Jones, M;Whalen, MJ;Symes, AJ;Georgakoudi, I;Nieland, TJF;Kaplan, DL;
    Cell death & disease
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Susceptibilities of CNS Cells towards Rabies Virus Infection Is Linked to Cellular Innate Immune Responses
    Authors: L Feige, T Kozaki, G Dias de Me, V Guillemot, F Larrous, F Ginhoux, H Bourhy
    Viruses, 2022-12-29;15(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Validation of Induced Microglia-Like Cells (iMG Cells) for Future Studies of Brain Diseases
    Authors: A Banerjee, Y Lu, K Do, T Mize, X Wu, X Chen, J Chen
    Frontiers in Cellular Neuroscience, 2021-04-09;15(0):629279.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Interleukin-34-CSF1R Signaling Axis Promotes Epithelial Cell Transformation and Breast Tumorigenesis
    Authors: M Poudel, G Kim, PY Bhattarai, JY Kim, HS Choi
    International Journal of Molecular Sciences, 2021-03-08;22(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Over-Expression of Allograft Inflammatory Factor-1 (AIF-1) in Patients with Rheumatoid Arthritis
    Authors: K Piotrowska, S S?uczanows, M Kurzawski, V Dziedziejk, P Kopytko, E Paczkowska, D Rogi?ska, K Safranow, B Machali?sk, A Pawlik
    Biomolecules, 2020-07-16;10(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  12. Neuroinflammation and EIF2 Signaling Persist despite Antiretroviral Treatment in an hiPSC Tri-culture Model of HIV Infection
    Authors: SK Ryan, MV Gonzalez, JP Garifallou, FC Bennett, KS Williams, NP Sotuyo, E Mironets, K Cook, H Hakonarson, SA Anderson, KL Jordan-Sci
    Stem Cell Reports, 2020-03-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Autocrine Production of Interleukin-34 Promotes the Development of Endometriosis through CSF1R/JAK3/STAT6 signaling
    Authors: K Lin, J Ma, Y Peng, M Sun, K Xu, R Wu, J Lin
    Sci Rep, 2019-11-14;9(1):16781.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Increased synapse elimination by microglia in schizophrenia patient-derived models of synaptic pruning
    Authors: CM Sellgren, J Gracias, B Watmuff, JD Biag, JM Thanos, PB Whittredge, T Fu, K Worringer, HE Brown, J Wang, A Kaykas, R Karmachary, CP Goold, SD Sheridan, RH Perlis
    Nat. Neurosci., 2019-02-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. slan+ monocytes and macrophages mediate CD20-dependent B cell lymphoma elimination via ADCC and ADCP
    Authors: W Vermi, A Micheletti, G Finotti, C Tecchio, F Calzetti, S Costa, M Bugatti, S Calza, C Agostinell, S Pileri, P Balzarini, A Tucci, G Rossi, L Furlani, G Todeschini, A Zamò, F Facchetti, L Lorenzi, S Lonardi, MA Cassatella
    Cancer Res., 2018-05-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Fibromyalgia and microglial TNF-?: Translational research using human blood induced microglia-like cells
    Authors: M Ohgidani, TA Kato, M Hosoi, M Tsuda, K Hayakawa, C Hayaki, R Iwaki, N Sagata, R Hashimoto, K Inoue, N Sudo, S Kanba
    Sci Rep, 2017-09-19;7(1):11882.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Interleukin-34 inhibits hepatitis B virus replication in vitro and in vivo
    Authors: ST Cheng, H Tang, JH Ren, X Chen, AL Huang, J Chen
    PLoS ONE, 2017-06-14;12(6):e0179605.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  18. IL-34 Upregulated Th17 Production through Increased IL-6 Expression by Rheumatoid Fibroblast-Like Synoviocytes
    Authors: B Wang, Z Ma, M Wang, X Sun, Y Tang, M Li, Y Zhang, F Li, X Li
    Mediators Inflamm., 2017-06-04;2017(0):1567120.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1alpha.
    Authors: Foucher E, Blanchard S, Preisser L, Descamps P, Ifrah N, Delneste Y, Jeannin P
    Eur J Immunol, 2015-01-19;45(4):1092-102.
    Species: Human
    Sample Types: Whole Cells
    Applications: Differentiation, Differentiation
  20. Direct induction of ramified microglia-like cells from human monocytes: dynamic microglial dysfunction in Nasu-Hakola disease.
    Authors: Ohgidani, Masahiro, Kato, Takahiro, Setoyama, Daiki, Sagata, Noriaki, Hashimoto, Ryota, Shigenobu, Kazue, Yoshida, Tetsuhik, Hayakawa, Kohei, Shimokawa, Norihiro, Miura, Daisuke, Utsumi, Hideo, Kanba, Shigenob
    Sci Rep, 2014-05-14;4(0):4957.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Cloning and expression of feline colony stimulating factor receptor (CSF-1R) and analysis of the species specificity of stimulation by colony stimulating factor-1 (CSF-1) and interleukin-34 (IL-34).
    Cytokine, 2012-12-19;61(2):630-8.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Bioassay

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