Recombinant Human LRFN5 Fc Chimera Protein, CF

R&D Systems | Catalog # 9385-SA

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems HEK293-derived Recombinant Human LRFN5 Fc Chimera Protein (9385-SA)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q96NI6

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all LRFN5 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LRFN5 protein
Human LRFN5
(Gln18-Gly527)
Accession # Q96NI6		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln18 inferred from enzymatic pyroglutamate treatment revealing Ile19

Predicted Molecular Mass

83 kDa

SDS-PAGE

90-107 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LRFN5 Fc Chimera is used at 1 μg/mL, the concentration of Recombinant Human LAR that produces 50% optimal binding response is 1.5-9 μg/mL.

Scientific Data Images for Recombinant Human LRFN5 Fc Chimera Protein, CF

Recombinant Human LRFN5 Fc Chimera Protein Bioactivity

Recombinant Human LRFN5 Fc Chimera Protein Bioactivity

When Recombinant Human LRFN5 Fc Chimera (Catalog # 9385-SA) is used at 1 µg/mL, 100 µL/well, Recombinant Human LAR (Catalog # 9377-PF) binds with an ED50 of 1.5-9 µg/mL.

Formulation, Preparation, and Storage

9385-SA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: LRFN5

Synaptic adhesion-like molecule 5 (SALM5; also leucine-rich repeat and fibronectin type-III domain-containing protein 5 (LRFN5)) is an approximately 90 kDa member of the SALM family of type I transmembrane glycoproteins (1). LRFNs comprise a family of synaptic adhesion molecules consisting of five members, each containing of an extracellular domain (ECD) of six leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly aligned in that order (1, 2).  LRFN-3 and -5 lack a C-terminal intracellular PDZ binding domain, which is conserved among LRFN-1, 2 and 4. Mature human LRFN-5 shares 99% amino acid sequence identity with mature mouse LRFNs. LRFN-5, like the other LRFNs, promotes neurite outgrowth as well as playing a role in neuroinflammation (3, 4). LRFN-5 is expressed in the brain and is capable of inducing presynaptic differentiation (5). Reduced expression of LRFN-5 has been associated with autism spectrum disorders and schizophrenia (6, 7). LAR family receptor protein tyrosine phosphatases (LAR-RPTPs) have been identified as novel ligands of LRFN-5 that mediates LRFN-5 dependent presynaptic differentiation in a splicing- dependent manner. LRFN-5 interacts directly with the Ig domain of LAR-RPTPs. The postsynaptic LRFN-5 promotes synapse development by trans-synaptically interacting with presynaptic  LAR-RPTPs which is important for the regulation of excitatory synaptic strength (8).

References

  1. Morimura, N. et al. (2006) Gene 380:72.
  2. Wang, C.-Y. et al. (2006) J. Neurosci. 26:2174.
  3. Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.
  4. Yuwen, Z. et al. (2106) Sci Adv. Apr;2(4).
  5. Choi, Y. et al. (2016) Sci Adv. Apr; 2(4).
  6. de Bruijn D.R.H. et al. (2010) Mol Syndromol 46.
  7. Xu, B. et al. (2009) PNAS 106:16746.
  8. Choi, Y. et.al. (2016) Sci Rep. 6:26676.

Long Name

Leucine-rich Repeat and Fibronectin Type III Domain Containing 5

Alternate Names

C14orf146, FIGLER8, SALM5

Entrez Gene IDs

145581 (Human); 238205 (Mouse); 314164 (Rat)

Gene Symbol

LRFN5

UniProt

Q96NI6

Additional LRFN5 Products

Product Documents for Recombinant Human LRFN5 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human LRFN5 Fc Chimera Protein, CF

For research use only

Related Research Areas

Immunoglobulin Superfamily CAMs

Customer Reviews for Recombinant Human LRFN5 Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human LRFN5 Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Human LRFN5 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes