Recombinant Human OTUD7B/Cezanne Catalytic Domain, CF

Name: Recombinant Human OTUD7B/Cezanne Catalytic Domain, CF
Brand: R&D Systems
SKU: E-563
Price: 226 USD

Catalog #: E-563
1 Citation Datasheet / COA / SDS

Discontinued Product

E-563 has been discontinued.

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Citations (1)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human OTUD7B/Cezanne Catalytic Domain, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Cezanne Catalytic Domain concentration of 0.1-1.0 μM.

Source

E. coli-derived human OTUD7B/Cezanne protein
Asn124 - Leu436

Accession #

Q6GQQ9

Predicted Molecular Mass

36 kDa

Product Datasheets

E-563

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-563

Formulation	1.8 mg/ml (50 μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10 % Glycerol (v/v), 1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: OTUD7B/Cezanne

OTU domain-containing protein 7B (OTUD7B), also known as cellular zinc finger anti-NF-kappa B (Cezanne), is a C64 type peptidase and a member of the ovarian tumor (OTU) protein super-family with a predicted molecular weight of 93 kDa. The human protein shares 90% amino acid sequence identity with its mouse ortholog. Cezanne has been reported to cleave K48-, and K63-linked polyubiquitin chains, but this enzyme displays a very strong preference for K11-linked chains in vitro. It has been shown to play a role in the negative regulation of NF-kappa B signaling, possibly by deubiquitination of RIPK1 and TRAF3. This untagged recombinant protein contains the catalytic domain (including the OTU domain) of Cezanne.

References

Bremm, A. et al. (2010) Nat. Struct. Mol. Biol. 17: 939
Enesa, K. et al. (2008) J. Biol. Chem. 283: 7036
Evans, P.C. et al. (2003) J. Biol. Chem. 278: 23180
Hu, H. et al. (2013) Nature 494: 371

Long Name

OTU Domain-containing Protein 7B

Entrez Gene IDs

56957 (Human); 229603 (Mouse); 310677 (Rat)

Alternate Names

cellular zinc finger NF-kappaB Cezanne; Cellular zinc finger NF-kappa-B protein; Cezanne; CEZANNEA20 domain containing 1; EC 3.4.19.12; OTU domain containing 7B; OTU domain-containing protein 7B; OTUD7B; ZA20D1; Zinc finger A20 domain-containing protein 1; Zinc finger protein Cezanne

Related Research Areas

Citation for Recombinant Human OTUD7B/Cezanne Catalytic Domain, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1