Recombinant Human Ret His-tag Protein, CF Summary
Product Specifications
When Recombinant Human Ret His-tag is immobilized at 1 µg/mL (100 µL/well), Recombinant Human GDNF (Catalog # 212-GD) binds with an ED50 of 0.05-0.3 μg/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha‑1 Fc Chimera (Catalog # 714-GR).
Leu29-Arg635, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1168-CR/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Background: Ret
The GDNF family of neurotrophic factors forms a subfamily within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and the adult animal. The GDNF family members (GDNF, neurturin, artemin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha -1 - 4). GFR alpha -1 -2, -3 and -4 are the preferred ligand-binding subunits for GDNF, neurturin, artemin and persephin, respectively. The Ret tyrosine-kinase receptor is encoded by the c-ret proto-oncogene. Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha (1-4).
- Trupp, M. et al. (1998) Mol. Cell. Neurosci. 11:47.
- Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
- Carlomagno, F. et al. (1998) Endocrinology 139:3613.
- Baloh, R. et al. (1998) Neuron 21:1291.
Citations for Recombinant Human Ret His-tag Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Long-term post-mortem studies following neurturin gene therapy in patients with advanced Parkinson's disease.
Authors: Chu Y, Bartus R, Manfredsson F, Olanow C, Kordower J
Brain, 2020-03-01;143(3):960-975.
Species: Mouse
Sample Types: Whole Tissue
Applications: Adsorption -
A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi.
Authors: Lu B, PereiraPerrin M
Infect. Immun., 2008-06-09;76(8):3530-8.
Species: Bacteria
Sample Types: Trypanosoma
Applications: PcIP
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