Recombinant Mouse CCL1/TCA-3 Protein

R&D Systems | Catalog # 845-TC

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse CCL1/TCA-3 Protein (845-TC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P10146

Applications

Bioactivity
View all CCL1/I-309/TCA-3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CCL1/I-309/TCA-3 protein
Lys24-Cys92

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys24

Predicted Molecular Mass

7.8 kDa

SDS-PAGE

13-16 kDa, reducing conditions

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR8.
The ED50 for this effect is 0.5-2.0 ng/mL.

Reviewed Applications

Read 2 reviews rated 4.5 using 845-TC in the following applications:

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 845-TC
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 845-TC/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL1/I-309/TCA-3

Mouse TCA-3 is a member of the CC beta family of chemokines. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.

TCA-3 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of TCA-3 contains one potential N-linked glycosylation site. Mouse TCA-3 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta and JE. TCA-3 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. TCA-3 has been shown to chemoattract T-cells.

References

  1. Goya, I. et al. (1998) J. Immunol. 160:1975.
  2. Burd, P. et al. (1987) J. Immunol. 139:3126.

Alternate Names

I-309, P500, SCYA1, SCYA2, TCA-3, TCA3

Entrez Gene IDs

6346 (Human); 20290 (Mouse)

Gene Symbol

CCL1

UniProt

P10146

Additional CCL1/I-309/TCA-3 Products

Product Documents for Recombinant Mouse CCL1/TCA-3 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 845-TC (with carrier)
Download SDS 845-TC/CF (carrier free)

Product Specific Notices for Recombinant Mouse CCL1/TCA-3 Protein

For research use only

Citations for Recombinant Mouse CCL1/TCA-3 Protein

Customer Reviews for Recombinant Mouse CCL1/TCA-3 Protein (2)

4.5 out of 5
2 Customer Ratings
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  • Recombinant Mouse CCL1/TCA-3 Protein
    Name: Anonymous
    Application: Characterization prior to use in downstream assays.
    Verified Customer | Posted 11/04/2019
    Recombinant Mouse CCL1/TCA-3 Protein 845-TC
  • Recombinant Mouse CCL1/TCA-3 Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 01/05/2018
    The recombinant TCA-3 protein was used as the standard with antibodies MAB8451 and BAF845 in an ELISA to measure mouse samples.
    Recombinant Mouse CCL1/TCA-3 Protein 845-TC

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