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Recombinant Mouse FGF-17 Protein

Catalog #: 7400-FG
1 Citation Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
7400-FG-025/CF

With Carrier

Catalog # Availability Size / Price Qty
7400-FG-025
R&D Systems Recombinant Proteins and Enzymes
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse FGF-17 Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED50 for this effect is 150‑750 ng/mL, in the presence of 10 µg/mL heparin.
Source
E. coli-derived mouse FGF-17 protein
Thr23-Thr216, with an N-terminal Met
Accession #
P63075
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
22.7 kDa
SDS-PAGE
23 kDa, reducing conditions  

Product Datasheets

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7400-FG (with carrier)

Product Datasheet
COA
SDS

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7400-FG/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7400-FG

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, (NH4)2SO4, DTT and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7400-FG/CF

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, (NH4)2SO4, DTT and EDTA.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-17

FGF‑17 is a member of the fibroblast growth factor (FGF) family. FGFs play multiple roles in biological functions, including angiogenesis, mitogenesis, cell differentiation and wound repair. FGFs share 30‑70% amino acid (aa) identity in a conserved, approximately 120 amino acid core domain (1‑3). The mouse or human FGF‑17 cDNA encodes a cleavable 22 aa signal sequence and a 194 secreted mature protein (1). Mature mouse FGF-17 shares 100%, 99%, 99%, 97%, and 97% aa identity with rat, human, porcine, canine and equine FGF‑17, respectively. The FGF domain of FGF‑17 shares the most aa identity with FGF-8 (75%) and FGF-18 (64%). These three FGFs constitute a subfamily that overlaps in some areas of expression and function (1‑5). All are reported to bind and signal through FGF R4 and the “c” splice forms of FGF R1-3 (6, 7). During embryogenesis, FGF‑17 plays an organizing and inducing role in the patterning at the midbrain/hindbrain junction, and is also expressed in hindgut, parts of the developing skeleton, tail bud, major arteries, and heart (2‑5). In many of these areas, it is expressed along with FGF-8, but slightly later (2‑6). Unlike FGF-8 and FGF‑18, deletion of FGF‑17 produces viable mice. However, FGF‑17-/- mice show abnormalities in the dorsal frontal cortex, midbrain and cerebellum, manifested in some cases by ataxia, auditory defects, and abnormal social behavior (1, 4, 5, 8, 9). In the adult, FGF-17 is expressed in ovarian follicles and the prostate, and its expression is increased by both benign hypertrophy and cancer of the prostate (10‑12). FGF‑8, FGF‑17, and FGF‑18 are also abnormally expressed in many leukemic cell lines and can enhance growth of cancer cells (13).

References
  1. Itoh, N. and D.M. Ornitz (2008) Dev. Dyn. 237:18.
  2. Maruoka, Y. et al. (1998) Mech. Dev. 74:175.
  3. Xu, J. et al. (1999) Mech. Dev. 83:165.
  4. Cholfin, J.A. and J.L.R. Rubenstein (2007) Proc. Natl. Acad. Sci. USA 104:7652.
  5. Xu, J. et al. (2000) Development 127:1833.
  6. Olsen, S.K. et al. (2006) Genes Dev. 20:185.
  7. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  8. Yu, X. et al. (2011) Neuroimage 56:1251.
  9. Scearce-Levie, K. et al. (2008) Genes Brain Behav. 7:344.
  10. Machado, M.F. et al. (2009) J. Endocrinol. 202:347.
  11. Polnaszek, N. et al. (2004) Prostate 60:18.
  12. Heer, R. et al. (2004) J. Pathol. 204:578.
  13. Nezu, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:843.
Long Name
Fibroblast Growth Factor 17
Entrez Gene IDs
8822 (Human); 14171 (Mouse)
Alternate Names
FGF-13; FGF17; FGF-17; fibroblast growth factor 17

Citation for Recombinant Mouse FGF-17 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Heparan sulphate sulphation by Hs2st restricts astroglial precursor somal translocation in developing mouse forebrain by a non cell autonomous mechanism
    Authors: JM Clegg, H Parkin, JO Mason, T Pratt
    J. Neurosci., 2019-01-07;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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