Recombinant Mouse SCF, Animal-Free Protein

Name: Recombinant Mouse SCF, Animal-Free Protein
Brand: R&D Systems
SKU: AFL455

Catalog #: AFL455 Datasheet / COA / SDS

Discontinued Product

AFL455 has been discontinued.

View all SCF/c-kit Ligand products.

R&D Systems Animal-Free Recombinant Proteins

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse SCF, Animal-Free Protein Summary

Animal Free Proteins
Animal-free proteins are particularly important for researchers concerned with experimental variables caused by trace animal components or mammalian pathogens. Our products generated under animal-free conditions share the same biological activities as those produced using our standard laboratory techniques.

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 2.5-10 ng/mL.

Source

E. coli-derived mouse SCF/c-kit Ligand protein
Lys26-Ala189, with an N-terminal Met
Produced using non-animal reagents in an animal-free laboratory.

Accession #

Q78ED8

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

18.6 kDa

Product Datasheets

AFL455

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AFL455

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: SCF/c-kit Ligand

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1 - 7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N-linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the short isoform (corresponding to this recombinant protein), mouse SCF shares 93% aa sequence identity with rat SCF and 72% - 75% with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (14). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling (15). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (16).

References

Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037.
Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599.
Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201.
Kapur, R. et al. (2002) Blood 100:1287.
Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039.
Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327.
Huang, E. et al. (1990) Cell 63:225.
Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. 88:4671.
Flanagan, J.G. et al. (1991) Cell 64:1025.
Martin, F.H. et al. (1990) Cell 63:203.
Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.

Long Name

Stem Cell Factor

Entrez Gene IDs

4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)

Alternate Names

c-kit Ligand; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SHEP7; SLF; steel factor

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.
Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.
Bulk proteins are filtered using animal-free filters.
Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

Low Endotoxin Level.
No impairment of biological activity.
High quality product obtained under stringent conditions.
For ex vivo research or bioproduction, additional documentation can be provided.

Please read our complete Animal-Free Statement