Recombinant Rabbit IL-4 Protein

Catalog #: 6939-RB Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
6939-RB-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
6939-RB-025

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rabbit IL-4 Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.

The ED₅₀for this effect is 3-15 ng/mL.

Source

E. coli-derived rabbit IL-4 protein
Arg25-Ser147, with an N-terminal Met

Accession #

NP_001156649

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

14.2 kDa

SDS-PAGE

12.5 kDa, reducing conditions

Product Datasheets

6939-RB (with carrier)

Product Datasheet

COA

SDS

6939-RB/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6939-RB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

6939-RB/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa ‑ 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1 ‑ 3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Rabbit IL-4 is synthesized with a 24 aa signal sequence (5). Mature rabbit IL-4 shares 47%, 56%, 39% and 40% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6 ‑ 8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2‑biased CD4⁺ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11 ‑ 14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15).

References

Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. 83:5894.
Redfield, C. et al. (1991) Biochemistry 30:11029.
Perkins, H.D. et al. (2000) Cytokine 12:555.
Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
Corthay, A. (2006) Scand. J. Immunol. 64:93.
Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

Long Name

Interleukin 4

Entrez Gene IDs

3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)

Alternate Names

B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra