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sCy5DA

Catalog #: 7834 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
7834/2
sCy5DA | Fluorescent Probes for Imaging Bacteria
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Description: FDAA for super-resolution microscopy of bacteria

Chemical Name: (R)-2-Ammonio-3-(6-((E)-3,3-dimethyl-5-sulfo-2-((2E,4E)-5-(1,3,3-trimethyl-5-sulfonato-3H-indol-1-ium-2-yl)penta-2,4-dien-1-ylidene)indolin-1-yl)hexanamido)propanoate

Purity: ≥95%

Product Details
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Biological Activity

sCy5DA is a fluorescent D-amino acid. It is suitable for labeling peptidoglycans in live bacteria. It is efficiently incorporated into the peptidoglycans of a wide variety of bacteria, including gram-positive, gram-negative, and mycobacteria. Incorporates particularly well into gram-negative C. crescentus. sCy5DA labelled bacteria can be imaged by single molecule localization microscopy (SMLM; also referred to as PALM or STORM). Excitation/emission maxima = 646/665 nm.

Scientific Data

Fluorescence Imaging Vibrio cholerae cells stained with sCy5DA View Larger

Single molecule localization microscopy (SMLM) image of Vibrio cholerae cells labeled with 500 μM sCy5DA

Fluorescence Imaging Bacillus subtilis cells stained with sCy5DA View Larger

Single molecule localization microscopy (SMLM) image of Bacillus subtilis cells labeled with 100 μM sCy5DA

Fluorescence Imaging Mycobacterium smegmatis cells stained with sCy5DA View Larger

Single molecule localization microscopy (SMLM) image of Mycobacterium smegmatis cells labeled with 100 μM sCy5DA

Fluorescence Imaging Staphylococcus aureus cells stained with sCy5DA View Larger

Single molecule localization microscopy (SMLM) image of Staphylococcus aureus labeled with 100 μM sCy5DA

Optical Data for sCy5DA

sCy5DA Dye Spectra
Emission Color Red
λabs 646 nm
λem 665 nm
Extinction Coefficient (ε) 197,240 M-1cm-1
Cell Permeable Yes
Application STORM

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data

M.Wt:
728.88
Formula:
C35H44N4O9S2
Solubility:
Soluble to 10 mM in DMSO
Purity:
≥95%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

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