Ubiquitin Conjugation Initiation Kit
Ubiquitin Conjugation Initiation Kit Summary
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Background: Ubiquitin
Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).
- Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
- Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
- Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
- Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
- Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
- Wei, W. et al. (2004) Nature 428:194.
- Wertz, I.E. et al. (2004) Nature 430:694.
Citations for Ubiquitin Conjugation Initiation Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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OTUB1 suppresses Hippo signaling via modulating YAP protein in gastric cancer
Authors: C Yan, H Yang, P Su, X Li, Z Li, D Wang, Y Zang, T Wang, Z Liu, Z Bao, S Dong, T Zhuang, J Zhu, Y Ding
Oncogene, 2022-10-21;0(0):. 2022-10-21
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The E3 ubiquitin ligase TRIM7 suppressed hepatocellular carcinoma progression by directly targeting Src protein
Authors: L Zhu, C Qin, T Li, X Ma, Y Qiu, Y Lin, D Ma, Z Qin, C Sun, X Shen, Y Zhao, L Han
Cell Death Differ., 2019-12-04;0(0):. 2019-12-04
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Modulation of M2 macrophage polarization by the crosstalk between Stat6 and Trim24
Authors: T Yu, S Gan, Q Zhu, D Dai, N Li, H Wang, X Chen, D Hou, Y Wang, Q Pan, J Xu, X Zhang, J Liu, S Pei, C Peng, P Wu, S Romano, C Mao, M Huang, X Zhu, K Shen, J Qin, Y Xiao
Nat Commun, 2019-09-25;10(1):4353. 2019-09-25
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CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer
Authors: Y Liu, X Zhang, J Lin, Y Chen, Y Qiao, S Guo, Y Yang, G Zhu, Q Pan, J Wang, F Sun
Cell Death Dis, 2019-09-09;10(9):644. 2019-09-09
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Regulation of a distinct activated RIPK1 intermediate bridging complex I and complex II in TNF?-mediated apoptosis
Authors: P Amin, M Florez, A Najafov, H Pan, J Geng, D Ofengeim, SA Dziedzic, H Wang, VJ Barrett, Y Ito, MJ LaVoie, J Yuan
Proc. Natl. Acad. Sci. U.S.A., 2018-06-11;115(26):E5944-E5953. 2018-06-11
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Keap1/Cullin3 Modulates p62/SQSTM1 Activity via UBA Domain Ubiquitination
Authors: Y Lee, TF Chou, SK Pittman, AL Keith, B Razani, CC Weihl
Cell Rep, 2017-04-04;19(1):188-202. 2017-04-04
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MARCH1 regulates insulin sensitivity by controlling cell surface insulin receptor levels
Nat Commun, 2016-08-31;7(0):12639. 2016-08-31
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Gsk3? and Tomm20 are substrates of the SCFFbxo7/PARK15 ubiquitin ligase associated with Parkinson's disease
Biochem J, 2016-08-08;0(0):. 2016-08-08
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Arabidopsis ubiquitin-conjugating enzyme UBC22 is required for female gametophyte development and likely involved in Lys11-linked ubiquitination
Authors: S Wang, L Cao, H Wang
J Exp Bot, 2016-04-10;0(0):. 2016-04-10
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Intracellular osteopontin stabilizes TRAF3 to positively regulate innate antiviral response
Authors: K Zhao, M Zhang, L Zhang, P Wang, G Song, B Liu, H Wu, Z Yin, C Gao
Sci Rep, 2016-03-30;6(0):23771. 2016-03-30
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RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR).
Authors: El Khouri E, Le Pavec G, Toledano M, Delaunay-Moisan A
J Biol Chem, 2013-09-09;288(43):31177-91. 2013-09-09
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