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Human IL-12 p70 ELISA Kit - Quantikine

Catalog #: D1200
73 Citations 4 Reviews Datasheet / COA / SDS
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S1200
PD1200
D1200
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QC01-1: Quantikine Immunoassay Control Group 1
Human IL-12 p70 ELISA Cell Culture Supernate/Serum/Plasma Standard Curve
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Citations (73)
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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IL-12 p70 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL), Urine (200 uL)
Sensitivity
5 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human IL-12p70
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma/Urine - Forty samples from apparently healthy volunteers were evaluated for the presence of human IL-12 p70 in this assay. No medical histories were available for the donors used in this study. All samples measured less than the lowest human IL-12 p70 standard, 7.8 pg/mL.

Cell Culture Supernates: NC-37 Burkitt's lymphoma B lymphoblast cells (1 x 106 cells/mL) were cultured for 3 days with and without 10 ng/mL PMA and 25 ng/mL calcium ionophore in RPMI 1640 media supplemented with 2 mM L-glutamine, 100 μg/mL penicillin, 100 μg/mL streptomycin, and 1% Nutridoma.

Human peripheral blood mononuclear cells (1 x 106 cells/mL) were cultured for 18 hours with and without 0.0075% (wt/vol) SAC (Pansorbin®, EMD Millipore) in RPMI 1640 media supplemented with 1% fetal bovine serum.

ConditionNC-37 cellsPBMCs
Stimulated8.3 pg/mL33 pg/mL
UnstimulatedNDND
ND=Non-detectable

Product Summary

The Quantikine Human IL-12 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-12 in cell culture supernates, serum, plasma, and urine. It contains Sf 21-expressed recombinant human IL-12 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-12 accurately. Results obtained using natural human IL-12 showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-12.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1
n 20 20 20 20
Mean (pg/mL) 21.2 228 447 21
Standard Deviation 0.3 3.5 4.9 1.5
CV% 1.4 1.5 1.1 7.1

Recovery

The recovery of human IL-12 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 90 81-96
Citrate Plasma (n=5) 90 82-94
EDTA Plasma (n=5) 101 96-109
Heparin Plasma (n=5) 99 89-109
Serum (n=5) 100 89-106
Urine (n=5) 89 85-92

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of IL-12 in various matrices and diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-12 p70 ELISA Linearity

Scientific Data

N/A Human IL-12 p70 ELISA Cell Culture Supernate/Serum/Plasma Standard Curve View Larger

Human IL-12 p70 ELISA Cell Culture Supernate/Serum/Plasma Standard Curve

N/A Human IL-12 p70 ELISA Urine Standard Curve View Larger

Human IL-12 p70 ELISA Urine Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-12 p70

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Long Name:
Interleukin 12 p70
Entrez Gene IDs:
3592 (Human); 16159 (Mouse); 84405 (Rat)
Alternate Names:
CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL12 p70; IL-12 p70; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, working standards, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL of the Appropriate Assay Diluent
  3.   Add 50 µL of the appropriate Assay Diluent to each well.
    4. 200 µL Standard, control, or sample
  4.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer.
  5.   For Cell Culture Supernate, Serum & Plasma Samples:  Incubate at room temperature for 2 hours.
    For Urine Samples:  Incubate at room temperature for 1.5 hours.
  6.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.
    7. 200 µL Conjugate
  7.   Add 200 µL of Conjugate to each well, and cover with a new plate sealer.
  8.   For Cell Culture Supernate, Serum & Plasma Samples:  Incubate at room temperature for 2 hours.
    For Urine Samples:  Incubate at room temperature for 1.5 hours.
  9.   Aspirate and wash 3 times.
    10. 200 µL Substrate Solution
  10. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
    11. 50 µL Stop Solution
  11. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IL-12 p70 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

73 Citations: Showing 1 - 10
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  1. A Comparative Analysis of Innate Immune Responses and the Structural Characterization of Spike from SARS-CoV-2 Gamma Variants and Subvariants
    Authors: Scovino, AM;Dahab, EC;Diniz-Lima, I;de Senna Silveira, E;Barroso, SPC;Cardoso, KM;Nico, D;Makhoul, GJ;da Silva-Junior, EB;Freire-de-Lima, CG;Freire-de-Lima, L;Fonseca, LMD;Valente, N;Nacife, V;Machado, A;Araújo, M;Vieira, GF;Pauvolid-Corrêa, A;Siqueira, M;Morrot, A;
    Microorganisms
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Unique Profile of Proinflammatory Cytokines in Plasma of Drug-Naïve Individuals with Advanced HIV/TB Co-Infection
    Authors: Nosik, M;Belikova, MG;Ryzhov, K;Avdoshina, D;Sobkin, A;Zverev, V;Svitich, O;
    Viruses
    Species: Human
    Sample Types: Plasma
  3. Combination IFNbeta and membrane-stable CD40L maximize tumor dendritic cell activation and lymph node trafficking to elicit systemic T-cell immunity
    Authors: H Zheng, X Yu, ML Ibrahim, D Foresman, M Xie, JO Johnson, TA Boyle, B Ruffell, BA Perez, SJ Antonia, N Ready, AN Saltos, MJ Cantwell, AA Beg
    Cancer Immunology Research, 2023-04-03;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. WT1 Pulsed Human CD141+ Dendritic Cell Vaccine Has High Potential in Solid Tumor-Targeted Immunotherapy
    Authors: SY Cho, SM Jeong, YJ Jeon, SJ Yang, JE Hwang, BM Yoo, HS Kim
    International Journal of Molecular Sciences, 2023-01-12;24(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Potential Protective Role of Galectin-3 in Patients with Gonarthrosis and Diabetes Mellitus: A Cross-Sectional Study
    Authors: J Milosevic, M Jurisevic, V Grbovic, I Jovanovic, N Gajovic, A Jurisic-Sk
    International Journal of Environmental Research and Public Health, 2022-09-13;19(18):.
    Species: Human
    Sample Types: Serum
  6. Mesothelin?specific T cell cytotoxicity against triple negative breast cancer is enhanced by 40s ribosomal protein subunit 3?treated self?differentiated dendritic cells
    Authors: N Jirapongwa, S Thongchot, W Chiraphapp, T Chieochans, D Sa-Nguanra, M Warnnissor, P Thuwajit, PT Yenchitsom, C Thuwajit
    Oncology reports, 2022-05-26;48(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Nanoplastics affect the inflammatory cytokine release by primary human monocytes and dendritic cells
    Authors: A Weber, A Schwiebs, H Solhaug, J Stenvik, AM Nilsen, M Wagner, B Relja, HH Radeke
    Environment international, 2022-03-15;163(0):107173.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Immunoregulatory effects of Lactococcus lactis-derived extracellular vesicles in allergic asthma
    Authors: DH Lee, HK Park, HR Lee, H Sohn, S Sim, HJ Park, YS Shin, YK Kim, Y Choi, HS Park
    Clinical and translational allergy, 2022-03-01;12(3):e12138.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Glioma Stem Cells Upregulate CD39 Expression to Escape Immune Response through SOX2 Modulation
    Authors: B Liu, Y Cao, Y Li, H Ma, M Yang, Q Zhang, G Li, K Zhang, Y Wu, Y Zhou, W Yang, T Sun
    Cancers, 2022-02-03;14(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Non-Clinical In Vitro Evaluation of Antibiotic Resistance Gene-Free Plasmids Encoding Human or Murine IL-12 Intended for First-in-Human Clinical Study
    Authors: S Kos, M Bosnjak, T Jesenko, B Markelc, U Kamensek, K Znidar, U Matkovic, A Rencelj, G Sersa, R Hudej, A Tuljak, M Peterka, M Cemazar
    Pharmaceutics, 2021-10-19;13(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Macrophages exert homeostatic actions in pregnancy to protect against preterm birth and fetal inflammatory injury
    Authors: N Gomez-Lope, V Garcia-Flo, PY Chin, HM Groome, MT Bijland, KR Diener, R Romero, SA Robertson
    JCI Insight, 2021-10-08;6(19):.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Association of Sarcopenia and Gut Microbiota Composition in Older Patients with Advanced Chronic Kidney Disease, Investigation of the Interactions with Uremic Toxins, Inflammation and Oxidative Stress
    Authors: E Margiotta, L Caldiroli, ML Callegari, F Miragoli, F Zanoni, S Armelloni, V Rizzo, P Messa, S Vettoretti
    Toxins, 2021-07-08;13(7):.
    Species: Human
    Sample Types: Serum
  13. Association between the uremic toxins indoxyl-sulfate and p-cresyl-sulfate with sarcopenia and malnutrition in elderly patients with advanced chronic kidney disease
    Authors: L Caldiroli, S Armelloni, A Eskander, P Messa, V Rizzo, E Margiotta, M Cesari, S Vettoretti
    Experimental gerontology, 2021-01-30;147(0):111266.
    Species: Human
    Sample Types: Serum
  14. Oxidized LDL Disrupts Metabolism and Inhibits Macrophage Survival by Activating a miR-9/Drp1/Mitochondrial Fission Signaling Pathway
    Authors: T Xin, C Lu, J Zhang, J Wen, S Yan, C Li, F Zhang, J Zhang
    Oxid Med Cell Longev, 2020-11-01;2020(0):8848930.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Comparative analysis of the tear protein profile in herpes simplex virus type 1 epithelial keratitis
    Authors: H Yang, X Yang, Y Wang, X Zheng, Y Zhang, Y Shao
    BMC Ophthalmol, 2020-08-31;20(1):355.
    Species: Human
    Sample Types: Tears
  16. Pro-inflammatory properties of H-ferritin on human macrophages, ex vivo and in vitro observations
    Authors: P Ruscitti, P Di Benedet, O Berardicur, N Panzera, N Grazia, AR Lizzi, P Cipriani, Y Shoenfeld, R Giacomelli
    Sci Rep, 2020-07-22;10(1):12232.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Gaps in Study Design for Immune Parameter Research for Latent Tuberculosis Infection: A Systematic Review
    Authors: M Herrera, C Vera, Y Keynan, ZV Rueda
    J Immunol Res, 2020-04-21;2020(0):8074183.
    Species: Human
    Sample Types: Plasma
  18. Dioscin attenuates oxLDL uptake and the inflammatory reaction of dendritic cells under high glucose conditions by blocking p38 MAPK
    Authors: Y Li, Y Li, T Yang, M Wang
    Mol Med Rep, 2019-11-06;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Structure establishment of three-dimensional (3D) cell culture printing model for bladder cancer
    Authors: MJ Kim, BH Chi, JJ Yoo, YM Ju, YM Whang, IH Chang
    PLoS ONE, 2019-10-22;14(10):e0223689.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Sarcopenia is Associated with Malnutrition but Not with Systemic Inflammation in Older Persons with Advanced CKD
    Authors: S Vettoretti, L Caldiroli, S Armelloni, C Ferrari, M Cesari, P Messa
    Nutrients, 2019-06-19;11(6):.
    Species: Human
    Sample Types: Serum
  21. Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging
    Authors: DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé
    JCI Insight, 2019-06-11;5(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Circulating growth/differentiation factor 15 is associated with human CD56bright natural killer cell dysfunction and nosocomial infection in severe systemic inflammation
    Authors: H Kleinertz, M Hepner-Sch, S Ehnert, M Claus, R Halbgebaue, L Boller, M Huber-Lang, P Cinelli, C Kirschning, S Flohé, A Sander, C Waydhas, S Vonderhage, M Jäger, M Dudda, C Watzl, SB Flohé
    EBioMedicine, 2019-04-13;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Plasma levels of TNF-?, IL-6, IFN-?, IL-12, IL-17, IL-22, and IL-23 in achalasia, eosinophilic esophagitis (EoE), and gastroesophageal reflux disease (GERD)
    Authors: S Clayton, E Cauble, A Kumar, N Patil, D Ledford, N Kolliputi, MF Lopes-Vire, D Castell, J Richter
    BMC Gastroenterol, 2019-02-11;19(1):28.
    Species: Human
    Sample Types: Plasma
  24. Mesenchymal stem cell transplantation ameliorates Sj�gren's syndrome via suppressing IL-12 production by dendritic cells
    Authors: B Shi, J Qi, G Yao, R Feng, Z Zhang, D Wang, C Chen, X Tang, L Lu, W Chen, L Sun
    Stem Cell Res Ther, 2018-11-08;9(1):308.
    Species: Human
    Sample Types: Serum
  25. Snail-overexpressing Cancer Cells Promote M2-Like Polarization of Tumor-Associated Macrophages by Delivering MiR-21-Abundant Exosomes
    Authors: CH Hsieh, SK Tai, MH Yang
    Neoplasia, 2018-07-02;20(8):775-788.
    Species: Human
    Sample Types: Exosomes
  26. Factors Influencing the Differentiation of Human Monocytic Myeloid-Derived Suppressor Cells Into Inflammatory Macrophages
    Authors: D Bayik, D Tross, DM Klinman
    Front Immunol, 2018-03-26;9(0):608.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Clinically Usable Interleukin 12 Plasmid without an Antibiotic Resistance Gene: Functionality and Toxicity Study in Murine Melanoma Model
    Authors: U Kamensek, N Tesic, G Sersa, M Cemazar
    Cancers (Basel), 2018-02-27;10(3):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  28. HCMV requires EGFR signaling to enter and initiate the early steps in the establishment of latency in CD34+ human progenitor cells
    Authors: Jung Heon Kim
    J. Virol, 2017-02-14;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations
    Authors: Y Wang, CS Ma, Y Ling, A Bousfiha, Y Camcioglu, S Jacquot, K Payne, E Crestani, R Roncagalli, A Belkadi, G Kerner, L Lorenzo, C Deswarte, M Chrabieh, E Patin, QB Vincent, I Müller-Fle, B Fleckenste, F Ailal, L Quintana-M, S Fraitag, MA Alyanakian, M Leruez-Vil, C Picard, A Puel, J Bustamante, S Boisson-Du, M Malissen, B Malissen, L Abel, A Hovnanian, LD Notarangel, E Jouanguy, SG Tangye, V Béziat, JL Casanova
    J. Exp. Med., 2016-09-19;213(11):2413-2435.
    Species: Human
    Sample Types: Whole Blood
  30. Defining the Pharmacodynamic Profile and Therapeutic Index of NHS-IL12 Immunocytokine in Dogs with Malignant Melanoma.
    Authors: Paoloni M, Mazcko C, Selting K, Lana S, Barber L, Phillips J, Skorupski K, Vail D, Wilson H, Biller B, Avery A, Kiupel M, LeBlanc A, Bernhardt A, Brunkhorst B, Tighe R, Khanna C
    PLoS ONE, 2015-06-19;10(6):e0129954.
    Species: Human
    Sample Types: Serum
  31. Live attenuated B. pertussis BPZE1 rescues the immune functions of Respiratory Syncytial virus infected human dendritic cells by promoting Th1/Th17 responses.
    Authors: Schiavoni I, Fedele G, Quattrini A, Bianco M, Schnoeller C, Openshaw P, Locht C, Ausiello C
    PLoS ONE, 2014-06-26;9(6):e100166.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Endocannabinoids affect innate immunity of Muller glia during HIV-1 Tat cytotoxicity.
    Authors: Krishnan G, Chatterjee N
    Mol Cell Neurosci, 2014-01-11;59(0):10-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Analysis of plasma cytokines and angiogenic factors in patients with pretreated urothelial cancer receiving Pazopanib: the role of circulating interleukin-8 to enhance the prognostic accuracy.
    Authors: Necchi A, Pennati M, Zaffaroni N, Landoni E, Giannatempo P, Raggi D, Schwartz L, Morosi C, Crippa F, Fare E, Nicolai N, Lanocita R, Sava T, Sacco C, Messina C, Ortega C, De Braud F, Salvioni R, Daidone M, Gianni A, Mariani L
    Br J Cancer, 2013-11-14;110(1):26-33.
    Species: Human
    Sample Types: Plasma
  34. Bile acids PKA-dependently induce a switch of the IL-10/IL-12 ratio and reduce proinflammatory capability of human macrophages.
    Authors: Haselow K, Bode J, Wammers M, Ehlting C, Keitel V, Kleinebrecht L, Schupp A, Haussinger D, Graf D
    J Leukoc Biol, 2013-08-29;94(6):1253-64.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Efficacy of oncolytic adenovirus expressing suicide genes and interleukin-12 in preclinical model of prostate cancer.
    Authors: Freytag, S O, Barton, K N, Zhang, Y
    Gene Ther, 2013-07-11;20(12):1131-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Effects of TLR agonists on maturation and function of 3-day dendritic cells from AML patients in complete remission.
    Authors: Beck B, Dorfel D, Lichtenegger FS, Geiger C, Lindner L, Merk M, Schendel DJ, Subklewe M
    J Transl Med, 2011-09-13;9(0):151.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. HDAC inhibition by LBH589 affects the phenotype and function of human myeloid dendritic cells.
    Authors: Song W, Tai YT, Tian Z, Hideshima T, Chauhan D, Nanjappa P, Exley MA, Anderson KC, Munshi NC
    Leukemia, 2010-11-19;25(1):161-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. Lack of anti-tumour reactivity despite enhanced numbers of circulating natural killer T cells in two patients with metastatic renal cell carcinoma.
    Authors: Vyth-Dreese FA, Sein J, van de Kasteele W, Dellemijn TA, van den Bogaard C, Nooijen WJ, de Gast GC, Haanen JB, Bex A
    Clin. Exp. Immunol., 2010-10-05;162(3):447-59.
    Species: Human
    Sample Types: Plasma
  39. CD14-independent responses induced by a synthetic lipid A mimetic.
    Authors: Legat A, Thomas S, Hermand P, Van Mechelen M, Goldman M, De Wit D
    Eur J Immunol, 2010-03-01;40(3):797-802.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Immunologic markers as predictors of tuberculosis-associated immune reconstitution inflammatory syndrome in HIV and tuberculosis coinfected persons in Thailand.
    Authors: Tieu HV, Ananworanich J, Avihingsanon A, Apateerapong W, Sirivichayakul S, Siangphoe U, Klongugkara S, Boonchokchai B, Hammer SM, Manosuthi W
    AIDS Res. Hum. Retroviruses, 2009-11-01;25(11):1083-9.
    Species: Human
    Sample Types: Serum
  41. Direct and indirect impairment of human dendritic cell function by virulent Francisella tularensis Schu S4.
    Authors: Chase JC, Celli J, Bosio CM
    Infect. Immun., 2008-11-03;77(1):180-95.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Cerebrospinal fluid levels of interleukin-6 and interleukin-12 in children with meningitis.
    Authors: Hsieh CC, Lu JH, Chen SJ, Lan CC, Chow WC, Tang RB
    Cytotechnology, 2008-09-25;25(4):461-5.
    Species: Human
    Sample Types: CSF
  43. In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22.
    Authors: Olsnes AM, Ryningen A, Ersvaer E, Bruserud Ø
    J. Interferon Cytokine Res., 2008-05-01;28(5):297-310.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
    Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
    Eur. J. Immunol., 2008-04-01;38(4):1012-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. Phase 2 study of pegylated liposomal doxorubicin in combination with interleukin-12 for AIDS-related Kaposi sarcoma.
    Authors: Little RF, Aleman K, Kumar P, Wyvill KM, Pluda JM, Read-Connole E, Wang V, Pittaluga S, Catanzaro AT, Steinberg SM, Yarchoan R
    Blood, 2007-09-10;110(13):4165-71.
    Species: Human
    Sample Types: Serum
  46. Translocation of bacterial DNA from Gram-positive microorganisms is associated with a species-specific inflammatory response in serum and ascitic fluid of patients with cirrhosis.
    Authors: Frances R, Gonzalez-Navajas JM, Zapater P, Munoz C, Cano R, Pascual S, Santana F, Marquez D, Perez-Mateo M, Such J
    Clin. Exp. Immunol., 2007-09-05;150(2):230-7.
    Species: Human
    Sample Types: Serum
  47. Fucoidan ingestion increases the expression of CXCR4 on human CD34+ cells.
    Authors: Irhimeh MR, Fitton JH, Lowenthal RM
    Exp. Hematol., 2007-06-01;35(6):989-94.
    Species: Human
    Sample Types: Plasma
  48. Chemokines CXCL10 and CXCL11 in the cerebrospinal fluid of patients with tick-borne encephalitis.
    Authors: Lepej SZ, Misic-Majerus L, Jeren T, Rode OD, Remenar A, Sporec V, Vince A
    Acta Neurol. Scand., 2007-02-01;115(2):109-14.
    Species: Human
    Sample Types: CSF
  49. IL-23 is increased in dendritic cells in multiple sclerosis and down-regulation of IL-23 by antisense oligos increases dendritic cell IL-10 production.
    Authors: Vaknin-Dembinsky A, Balashov K, Weiner HL
    J. Immunol., 2006-06-15;176(12):7768-74.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Bordetella pertussis inhibition of interleukin-12 (IL-12) p70 in human monocyte-derived dendritic cells blocks IL-12 p35 through adenylate cyclase toxin-dependent cyclic AMP induction.
    Authors: Spensieri F, Fedele G, Fazio C, Nasso M, Stefanelli P, Mastrantonio P, Ausiello CM
    Infect. Immun., 2006-05-01;74(5):2831-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. Celecoxib up-regulates the expression of the zeta chain of T cell receptor complex in tumor-infiltrating lymphocytes in human cervical cancer.
    Authors: Ferrandina G, Ranelletti FO, Legge F, Salutari V, Martinelli E, Fattorossi A, Lorusso D, Zannoni G, Vellone V, Paglia A, Scambia G
    Clin. Cancer Res., 2006-04-01;12(7):2055-60.
    Species: Human
    Sample Types: Plasma
  52. Elevation of IL-12 p40 and its antibody in myasthenia gravis with thymoma.
    Authors: Yoshikawa H, Sato K, Edahiro S, Furukawa Y, Maruta T, Iwasa K, Watanabe H, Takaoka S, Suzuki Y, Takamori M, Yamada M
    J. Neuroimmunol., 2006-03-29;175(1):169-75.
    Species: Human
    Sample Types: Serum
  53. Chemokines indicate allergic bronchopulmonary aspergillosis in patients with cystic fibrosis.
    Authors: Hartl D, Latzin P, Zissel G, Krane M, Krauss-Etschmann S, Griese M
    Am. J. Respir. Crit. Care Med., 2006-03-16;173(12):1370-6.
    Species: Human
    Sample Types: Serum
  54. C-reactive protein and vascular cell adhesion molecule-1 as markers of severity in sickle cell disease.
    Authors: Makis AC, Hatzimichael EC, Stebbing J, Bourantas KL
    Arch. Intern. Med., 2006-02-13;166(3):366-8.
    Species: Human
    Sample Types: Plasma
  55. Interleukin-12 and interleukin-16 in periodontal disease.
    Authors: Tsai IS, Tsai CC, Ho YP, Ho KY, Wu YM, Hung CC
    Cytokine, 2005-07-07;31(1):34-40.
    Species: Human
    Sample Types:
  56. Effect of piceatannol in human monocyte-derived dendritic cells in vitro.
    Authors: Takei M, Umeyama A, Arihara S, Matsumoto H
    J Pharm Sci, 2005-05-01;94(5):974-82.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. Manipulation of host cytokine network by ticks: a potential gateway for pathogen transmission.
    Authors: Hajnicka V, Vancova I, Kocakova P, Slovak M, Gasperik J, Slavikova M, Hails RS, Labuda M, Nuttall PA
    Parasitology, 2005-03-01;130(0):333-42.
    Species: Human
    Sample Types: Saliva
  58. Cytokines and adhesion molecules in multiple sclerosis patients treated with interferon-beta1b.
    Authors: Jensen J, Krakauer M, Sellebjerg F
    Cytokine, 2005-01-07;29(1):24-30.
    Species: Human
    Sample Types: Plasma
  59. Human plasmacytoid dendritic cells activated by CpG oligodeoxynucleotides induce the generation of CD4+CD25+ regulatory T cells.
    Authors: Moseman EA, Liang X, Dawson AJ, Panoskaltsis-Mortari A, Krieg AM, Liu YJ, Blazar BR, Chen W
    J. Immunol., 2004-10-01;173(7):4433-42.
    Species: Human
    Sample Types: Cell Culture Supernates
  60. Effects of antiasthmatic agents on the functions of peripheral blood monocyte-derived dendritic cells from atopic patients.
    Authors: Saeki S, Matsuse H, Kondo Y, Machida I, Kawano T, Tomari S, Obase Y, Fukushima C, Kohno S
    J. Allergy Clin. Immunol., 2004-09-01;114(3):538-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. Production of interferon gamma in respiratory syncytial virus infection of humans is not associated with interleukins 12 and 18.
    Authors: Garofalo RP, Hintz KH, Hill V, Ogra PL, Welliver RC
    J. Med. Virol., 2004-06-01;73(2):289-94.
    Species: Human
    Sample Types: Tissue Secretion
  62. Lack of phenotypic and functional impairment in dendritic cells from chimpanzees chronically infected with hepatitis C virus.
    Authors: Larsson M, Babcock E, Grakoui A, Shoukry N, Lauer G, Rice C, Walker C, Bhardwaj N
    J. Virol., 2004-06-01;78(12):6151-61.
    Species: Primate - Pan troglodytes (Chimpanzee)
    Sample Types: Cell Culture Supernates
  63. Cytokines evaluation in nasal lavage of allergic children after Bacillus clausii administration: a pilot study.
    Authors: Ciprandi G, Tosca MA, Milanese M, Caligo G, Ricca V
    Pediatr Allergy Immunol, 2004-04-01;15(2):148-51.
    Species: Human
    Sample Types: Nasal Lavage Fluid
  64. Inflammatory cytokine concentrations are elevated in seminal plasma of men with spinal cord injuries.
    Authors: Basu S, Aballa TC, Ferrell SM, Lynne CM, Brackett NL
    J. Androl., 2004-03-01;25(2):250-4.
    Species: Human
    Sample Types: Seminal Plasma
  65. Reduced levels of transforming growth factor-beta1, interleukin-12 and increased migration inhibitory factor are associated with severe malaria.
    Authors: Chaiyaroj SC, Rutta AS, Muenthaisong K, Watkins P, Na Ubol M, Looareesuwan S
    Acta Trop., 2004-02-01;89(3):319-27.
    Species: Human
    Sample Types: Plasma
  66. NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen.
    Authors: Vankayalapati R, Klucar P, Wizel B, Weis SE, Samten B, Safi H, Shams H, Barnes PF
    J. Immunol., 2004-01-01;172(1):130-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  67. HIV-1 protein Vpr suppresses IL-12 production from human monocytes by enhancing glucocorticoid action: potential implications of Vpr coactivator activity for the innate and cellular immunity deficits observed in HIV-1 infection.
    Authors: Mirani M, Elenkov I, Volpi S, Hiroi N, Chrousos GP, Kino T
    J. Immunol., 2002-12-01;169(11):6361-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  68. Intraperitoneal bispecific antibody (HEA125xOKT3) therapy inhibits malignant ascites production in advanced ovarian carcinoma.
    Authors: Marme A, Strauss G, Bastert G, Grischke EM, Moldenhauer G
    Int. J. Cancer, 2002-09-10;101(2):183-9.
    Species: Human
    Sample Types: Ascites Fluid
  69. Local immunostimulation induced by intravesical administration of autologous interferon-gamma-activated macrophages in patients with superficial bladder cancer.
    Authors: Pages F, Lebel-Binay S, Vieillefond A, Deneux L, Cambillau M, Soubrane O, Debre B, Tardy D, Lemonne JL, Abastado JP, Fridman WH, Thiounn N
    Clin. Exp. Immunol., 2002-02-01;127(2):303-9.
    Species: Human
    Sample Types: Urine
  70. Cytokine profile in Behcet's disease patients. Relationship with disease activity.
    Authors: Hamzaoui K, Hamzaoui A, Guemira F, Bessioud M, Hamza M, Ayed K
    Scand. J. Rheumatol., 2002-01-01;31(4):205-10.
    Species: Human
    Sample Types: Serum
  71. Mature dendritic cells infiltrate the T cell-rich region of oral mucosa in chronic periodontitis: in situ, in vivo, and in vitro studies.
    Authors: Jotwani R, 175, Palucka AK, Al-Quotub M, Nouri-Shirazi M, Kim J, Bell D, Banchereau J, Cutler CW
    2001-10-15;167(8):4693-700.
    Species: Human
    Sample Types:
  72. Interleukin-11 induces Th2 polarization of human CD4(+) T cells.
    Authors: Curti A, Ratta M, Corinti S, Girolomoni G, Ricci F, Tazzari P, Siena M, Grande A, Fogli M, Tura S, Lemoli RM
    Blood, 2001-05-01;97(9):2758-63.
    Species: Human
    Sample Types: Cell Culture Supernates
  73. Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
    Authors: Kadowaki N, Antonenko S, Lau JY, Liu YJ
    J. Exp. Med., 2000-07-17;192(2):219-26.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human IL-12 p70 Quantikine ELISA Kit
By Anonymous on 02/25/2023
Sample Tested: Serum and Plasma
Human IL-12 p70 Quantikine ELISA Kit
By Anonymous on 02/25/2023
Sample Tested: Serum-free Cell Culture Supernates
Human IL-12 p70 Quantikine ELISA Kit
By komal patel on 07/25/2020
Sample Tested: Porcine Serum,Serum
Human IL-12 p70 Quantikine ELISA Kit D1200
Human IL-12 p70 Quantikine ELISA Kit
By Anonymous on 05/11/2020
Sample Tested: macrophage