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Human ICAM-1/CD54 Allele-specific ELISA Kit - Quantikine

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Human ICAM-1/CD54 ELISA Standard Curve
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
2 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.254 ng/mL
Assay Range
1.6 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human sICAM-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human sICAM-1 immunoassay is a 2.0 hour solid phase ELISA designed to measure sICAM-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human sICAM-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human sICAM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring sICAM-1.

Precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 2 3
n 40 40
Mean (ng/mL) 12.5 20.5
Standard Deviation 0.67 0.9
CV% 5.4 4.4

Recovery

The recovery of sICAM-1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 104 90-109

Linearity

To assess the linearity of the assay, samples spiked with or containing high concentrations of sICAM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human ICAM-1/CD54 ELISA Linearity

Scientific Data

N/A Human ICAM-1/CD54 ELISA Standard Curve View Larger

Human ICAM-1/CD54 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ICAM-1/CD54

ICAM-1 (intercellular adhesion molecule-1), also known as CD54, is a transmembrane protein that is upregulated on endothelial and epithelial cells at sites of inflammation. It mediates the vascular adhesion and paracellular migration of leukocytes expressing activated LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). It also binds several non-integrin ligands including CD43/Sialophorin, Fibrinogen, Hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes. Soluble ICAM-1 promotes angiogenesis and serves an indicator of vascular endothelial cell activation or damage. Elevated levels of soluble ICAM-1 are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, increased abdominal fat mass, hypertension, liver disease, certain malignancies, and cerebral malaria.

Long Name:
Intercellular Adhesion Molecule 1
Entrez Gene IDs:
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names:
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Conjugate
  3.   Add 100 µL of Conjugate to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1.5 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Substrate Solution
  6.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    7. 50 µL Stop Solution
  7.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

72 Citations: Showing 1 - 10
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  1. CD40 Ligand-CD40 Interaction Is an Intermediary between Inflammation and Angiogenesis in Proliferative Diabetic Retinopathy
    Authors: Abu El-Asrar, AM;Nawaz, MI;Ahmad, A;Dillemans, L;Siddiquei, M;Allegaert, E;Gikandi, PW;De Hertogh, G;Opdenakker, G;Struyf, S;
    International journal of molecular sciences
    Species: Human
    Sample Types: Vitreous Humor
  2. Decreased Serum Stromal Cell-Derived Factor-1 in Patients with Familial Hypercholesterolemia and Its Strong Correlation with Lipoprotein Subfractions
    Authors: Juhász, L;L?rincz, H;Szentpéteri, A;Tóth, N;Varga, É;Paragh, G;Harangi, M;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  3. Lung Ultrasound Findings and Endothelial Perturbation in a COVID-19 Low-Intensity Care Unit
    Authors: R Gualtierot, F Tafuri, R Rossio, M Rota, P Bucciarell, B Ferrari, A Giachi, C Suffritti, M Cugno, F Peyvandi, On Behalf
    Journal of Clinical Medicine, 2022-09-15;11(18):.
    Species: Human
    Sample Types: Serum
  4. Hepatocyte Nuclear Factor 1alpha Proinflammatory Effect Linked to the Overexpression of Liver Nuclear Factor-kappaB in Experimental Model of Chronic Kidney Disease
    Authors: E Sucajtys-S, A Debska-Sli, B Rutkowski, M Szolkiewic, J Swierczyns, RT Smolenski
    International Journal of Molecular Sciences, 2022-08-10;23(16):.
    Species: Rat
    Sample Types: Serum
  5. Oxidative stress-induced endothelial dysfunction and decreased vascular nitric oxide in COVID-19 patients
    Authors: V Montiel, I Lobysheva, L Gérard, M Vermeersch, D Perez-Morg, T Castelein, JB Mesland, P Hantson, C Collienne, D Gruson, MA van Dievoe, A Persu, C Beauloye, M Dechamps, L Belkhir, A Robert, M Derive, PF Laterre, AHJ Danser, X Wittebole, JL Balligand
    EBioMedicine, 2022-02-23;77(0):103893.
    Species: Human
    Sample Types: Serum
  6. Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study
    Authors: C Gar, B Thorand, C Herder, C Sujana, M Heier, C Meisinger, A Peters, W Koenig, W Rathmann, M Roden, M Stumvoll, H Maalmi, T Meitinger, H Then, J Seissler, C Then
    PLoS ONE, 2022-01-06;17(1):e0262330.
    Species: Human
    Sample Types: Serum
  7. Effect of the Renin-Angiotensin-Aldosterone System Reactivity on Endothelial Function and Modulative Role of Valsartan in Male Subjects with Essential Hypertension
    Authors: J Jasiczek, M Trocha, A Derkacz, E Szahidewic, A Doroszko
    Journal of Clinical Medicine, 2021-12-13;10(24):.
    Species: Human
    Sample Types: Plasma
  8. Differences in the Biomarker Profile of De Novo Acute Heart Failure versus Decompensation of Chronic Heart Failure
    Authors: S Nawrocka-M, J Biegus, M Hurkacz, M Guzik, M Rosiek-Bie, EA Jankowska, P Ponikowski, R Zymli?ski
    Biomolecules, 2021-11-16;11(11):.
    Species: Human
    Sample Types: Plasma
  9. Relationship of low molecular weight fluorophore levels with clinical factors and fenofibrate effects in adults with type 2 diabetes
    Authors: AS Januszewsk, D Chen, RS Scott, RL O'Connell, NR Aryal, DR Sullivan, GF Watts, MR Taskinen, PJ Barter, JD Best, RJ Simes, AC Keech, AJ Jenkins
    Scientific Reports, 2021-09-21;11(1):18708.
    Species: Human
    Sample Types: Serum
  10. Preterm Intraventricular Hemorrhage-Induced Inflammatory Response in Human Choroid Plexus Epithelial Cells
    Authors: Z Fejes, M Pócsi, J Takai, J Erdei, A Tóth, E Balogh, Á Rusznyák, F Fenyvesi, A Nagy, J Kappelmaye, V Jeney, B Nagy
    International Journal of Molecular Sciences, 2021-08-11;22(16):.
    Species: Human
    Sample Types: CSF
  11. Serum Metabolomic Analysis of Coronary Heart Disease Patients with Stable Angina Pectoris Subtyped by Traditional Chinese Medicine Diagnostics Reveals Biomarkers Relevant to Personalized Treatments
    Authors: N Guo, P Wang, J Yang, X Yang, M van der Vo, M Wildwater, J Wei, X Tang, M Wang, H Yang
    Frontiers in Pharmacology, 2021-06-14;12(0):664320.
    Species: Human
    Sample Types: Serum
  12. The Short-Term Kinetics of sICAM-1 after Induction of Acute Experimental Pain in Healthy Volunteers
    Authors: P Lüke, E Kraft, SC Azad
    Journal of Clinical Medicine, 2021-05-09;10(9):.
    Species: Human
    Sample Types: Plasma
  13. Endothelial glycocalyx degradation and disease severity in Plasmodium vivax and Plasmodium knowlesi malaria
    Authors: BE Barber, MJ Grigg, KA Piera, Y Chen, T William, JB Weinberg, TW Yeo, NM Anstey
    Scientific Reports, 2021-05-07;11(1):9741.
    Species: Human
    Sample Types: Plasma
  14. The effects of exercise training on lipid profile in patients with sarcoidosis
    Authors: D Jastrzebsk, B Toczylowsk, E Zieminska, A Zebrowska, S Kostorz-No, E Swietochow, C Di Giulio, D Ziora
    Scientific Reports, 2021-03-10;11(1):5551.
    Species: Human
    Sample Types: Serum
  15. Leptin mediate central obesity on the severity of cardiovascular autonomic neuropathy in well-controlled type 2 diabetes and prediabetes
    Authors: YR Lai, MH Chen, WC Lin, WC Chiu, BC Cheng, JF Chen, NW Tsai, CC Huang, CH Lu
    J Transl Med, 2020-10-19;18(1):396.
    Species: Human
    Sample Types: Serum
  16. Serum and CSF cytokine levels mirror different neuroimmunological mechanisms in patients with LGI1 and Caspr2 encephalitis
    Authors: P Körtvelyes, A Goihl, K Guttek, B Schraven, H Prüss, D Reinhold
    Cytokine, 2020-08-12;135(0):155226.
    Species: Human
    Sample Types: Serum
  17. Blood Monocyte Phenotype Fingerprint of Stable Coronary Artery Disease: A Cross-Sectional Substudy of SMARTool Clinical Trial
    Authors: S Sbrana, J Campolo, A Clemente, L Bastiani, A Cecchettin, E Ceccherini, C Caselli, D Neglia, O Parodi, D Chiappino, JM Smit, AJ Scholte, G Pelosi, S Rocchiccio
    Biomed Res Int, 2020-07-27;2020(0):8748934.
    Species: Human
    Sample Types: Plasma
  18. The association between metabolic components and markers of inflammatory and endothelial dysfunction in adolescents, based on the Ewha Birth and Growth Cohort Study
    Authors: HA Lee, EJ Choi, B Park, H Lee, YS Hong, HS Kim, MK Shin, H Park
    PLoS ONE, 2020-05-20;15(5):e0233469.
    Species: Human
    Sample Types: Serum
  19. HIV antiretroviral drugs, dolutegravir, maraviroc and ritonavir-boosted atazanavir use different pathways to affect inflammation, senescence and insulin sensitivity in human coronary endothelial cells
    Authors: M Auclair, AC Guénantin, S Fellahi, M Garcia, J Capeau
    PLoS ONE, 2020-01-23;15(1):e0226924.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Markers of endothelial cell activation and neutrophil extracellular traps are elevated in immune thrombocytopenia but are not enhanced by thrombopoietin receptor agonists
    Authors: L Garabet, CE Henriksson, ML Lozano, W Ghanima, J Bussel, E Brodin, MP Fernández-, C Martínez, R González-C, MC Mowinckel, PM Sandset
    Thromb. Res., 2019-11-30;185(0):119-124.
    Species: Human
    Sample Types: Plasma
  21. Serum and cerebrospinal fluid Neudesin concentration and Neudesin Quotient as potential circulating biomarkers of a primary brain tumor
    Authors: OM Koper-Lenk, J Kami?ska, A Milewska, K Sawicki, M Jadeszko, Z Mariak, J Resze?, V Dymicka-Pi, J Matowicka-
    BMC Cancer, 2019-04-05;19(1):319.
    Species: Human
    Sample Types: Serum
  22. Low-Dose Carbon Monoxide Inhibits Rhinovirus Replication in Human Alveolar and Airway Epithelial Cells
    Authors: X Deng, H Yasuda, T Sasaki, M Yamaya
    Tohoku J. Exp. Med., 2019-04-01;247(4):215-222.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. CD34+ and CD34+VEGFR2+?cells in poorly controlled hypertensive patients
    Authors: MW Skrzypkows, PG Gutknecht, ME Ryba-Stani, B S?omi?ski, J Siebert, JM My?liwska
    J Hum Hypertens, 2018-12-19;0(0):.
    Species: Human
    Sample Types: Serum
  24. Lp-PLA2, scavenger receptor class B type I gene (SCARB1) rs10846744 variant, and cardiovascular disease
    Authors: A Manichaiku, XQ Wang, L Li, J Erdmann, G Lettre, JC Bis, D Waterworth, M Cushman, NS Jenny, WS Post, W Palmas, MY Tsai, L Wallentin, H White, H Schunkert, CJ O'Donnell, DM Herrington, SS Rich, ML O'Donoghue, A Rodriguez
    PLoS ONE, 2018-10-05;13(10):e0204352.
    Species: Human
    Sample Types: Plasma
  25. Effect of Combined Treatment With Folic Acid, Vitamin B6, and Vitamin B12 on Plasma Biomarkers of Inflammation and Endothelial Dysfunction in Women
    Authors: WG Christen, NR Cook, M Van Denbur, E Zaharris, CM Albert, JE Manson
    J Am Heart Assoc, 2018-05-18;7(11):.
    Species: Human
    Sample Types: Plasma
  26. Targeted endothelial gene deletion of Triggering Receptor Expressed on Myeloid cells-1 protects mice during septic shock
    Authors: L Jolly, K Carrasco, M Derive, J Lemarié, A Boufenzer, S Gibot
    Cardiovasc. Res., 2018-05-01;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Identification of sarilumab pharmacodynamic and predictive markers in patients with inadequate response to TNF inhibition: a biomarker substudy of the phase 3 TARGET study
    Authors: C Gabay, J Msihid, M Zilberstei, C Paccard, Y Lin, NMH Graham, A Boyapati
    RMD Open, 2018-03-14;4(1):e000607.
    Species: Human
    Sample Types: Serum
  28. Association of circulating CTRP9 with soluble adhesion molecules and inflammatory markers in patients with type 2 diabetes mellitus and coronary artery disease
    Authors: N Moradi, R Fadaei, S Emamgholip, E Kazemian, G Panahi, S Vahedi, L Saed, S Fallah
    PLoS ONE, 2018-01-30;13(1):e0192159.
    Species: Human
    Sample Types: Serum
  29. PM2.5-induced oxidative stress increases intercellular adhesion molecule-1 expression in lung epithelial cells through the IL-6/AKT/STAT3/NF-?B-dependent pathway
    Authors: CW Liu, TL Lee, YC Chen, CJ Liang, SH Wang, JH Lue, JS Tsai, SW Lee, SH Chen, YF Yang, TY Chuang, YL Chen
    Part Fibre Toxicol, 2018-01-12;15(1):4.
    Species: Human
    Sample Types: Plasma
  30. Antioxidants from diet or supplements do not alter inflammatory markers in adults with cardiovascular disease risk. A pilot randomized controlled trial
    Authors: A Dewell, P Tsao, J Rigdon, CD Gardner
    Nutr Res, 2017-11-02;50(0):63-72.
    Species: Human
    Sample Types: Whole Cells
  31. Cerebrospinal fluid and serum IL-8, CCL2, and ICAM-1 concentrations in astrocytic brain tumor patients.
    Authors: Koper O, Kaminska J, Sawicki K, Reszec J, Rutkowski R, Jadeszko M, Mariak Z, Dymicka-Piekarska V, Kemona H
    Ir J Med Sci, 2017-10-30;0(0):.
    Species: Human
    Sample Types: Serum
  32. Influence of ethnic background on left atrial markers of inflammation, endothelial function and tissue remodelling
    Authors: CD Ruediger, B John, S Kumar, HS Lim, G Rangnekar, KC Roberts-Th, GD Young, D Chase, P Sanders, SR Willoughby
    Indian Pacing Electrophysiol J, 2017-08-30;18(1):1-5.
    Species: Human
    Sample Types: Serum
  33. Paraoxonase-1 and myeloperoxidase correlate with vascular biomarkers in overweight patients with newly diagnosed untreated hyperlipidaemia
    Authors: A Szentpéter, N Zsíros, VE Varga, H L?rincz, M Katkó, I Seres, P Fülöp, G Paragh, M Harangi
    VASA, 2017-06-12;0(0):1-7.
    Species: Human
    Sample Types: Serum
  34. Altered Monocyte and Endothelial Cell Adhesion Molecule Expression Is Linked to Vascular Inflammation in Human Immunodeficiency Virus Infection
    Authors: M Kulkarni, E Bowman, J Gabriel, T Amburgy, E Mayne, DA Zidar, C Maierhofer, AN Turner, JA Bazan, SL Koletar, MM Lederman, SF Sieg, NT Funderburg
    Open Forum Infect Dis, 2016-10-15;3(4):ofw224.
    Species: Human
    Sample Types: Plasma
  35. Serum Interleukin-18, Fetuin-A, Soluble Intercellular Adhesion Molecule-1, and Endothelin-1 in Ankylosing Spondylitis, Psoriatic Arthritis, and SAPHO Syndrome
    Int J Mol Sci, 2016-08-03;17(8):.
    Species: Human
    Sample Types: Serum
  36. Immune-inflammatory markers and arterial stiffness indexes in subjects with acute ischemic stroke with and without metabolic syndrome.
    Authors: Tuttolomondo A, Pecoraro R, Di Raimondo D, Di Sciacca R, Canino B, Arnao V, Butta C, Della Corte V, Maida C, Licata G, Pinto A
    Diabetol Metab Syndr, 2014-02-27;6(1):28.
    Species: Human
    Sample Types: Plasma
  37. Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study.
    Authors: Teichert T, Vossoughi M, Vierkotter A, Sugiri D, Schikowski T, Schulte T, Roden M, Luckhaus C, Herder C, Kramer U
    PLoS ONE, 2013-12-10;8(12):e83042.
    Species: Human
    Sample Types: Plasma
  38. Plasma concentrations predict aortic expression of growth-arrest-specific protein 6 in patients undergoing coronary artery bypass grafting.
    Authors: Lee, Chien-Hs, Shieh, Yi-Shing, Tsai, Chien-Su, Hung, Yi-Jen, Tsai, Yi-Ting, Lin, Chih-Yua
    PLoS ONE, 2013-11-13;8(11):e79452.
    Species: Human
    Sample Types: Plasma
  39. Biomarker results from the AVADO phase 3 trial of first-line bevacizumab plus docetaxel for HER2-negative metastatic breast cancer.
    Authors: Miles D, de Haas S, Dirix L, Romieu G, Chan A, Pivot X, Tomczak P, Provencher L, Cortes J, Delmar P, Scherer S
    Br J Cancer, 2013-02-19;108(5):1052-60.
    Species: Human
    Sample Types: Plasma
  40. Role of serum concentration of VEGFR1 and TIMP2 on clinical outcome in primary cervical cancer: results of a companion protocol of the randomized, NOGGO-AGO phase III adjuvant trial of simultaneous cisplatin-based radiochemotherapy vs. carboplatin and paclitaxel containing sequential radiotherapy.
    Authors: Braicu E, Fotopoulou C, Chekerov R, Richter R, Blohmer J, Kummel S, Stamatian F, Yalcinkaya I, Mentze M, Lichtenegger W, Sehouli J
    Cytokine, 2013-02-14;61(3):755-8.
    Species: Human
    Sample Types: Serum
  41. Suppression of NF-kappaB Reduces Myocardial No-Reflow.
    Authors: Zeng M, Yan H, Chen Y, Zhao H, Lv Y, Liu C, Zhou P, Zhao B
    PLoS ONE, 2012-10-09;7(10):e47306.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Cerebrospinal Fluid Neopterin as Marker of the Meningo-Encephalitic Stage of Trypanosoma brucei gambiense Sleeping Sickness.
    Authors: Tiberti N, Hainard A, Lejon V, Courtioux B, Matovu E, Enyaru JC, Robin X, Turck N, Kristensson K, Ngoyi DM, Vatunga GM, Krishna S, Buscher P, Bisser S, Ndung'u JM, Sanchez JC
    PLoS ONE, 2012-07-18;7(7):e40909.
    Species: Human
    Sample Types: CSF
  43. Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis.
    BMC Infect Dis, 2012-06-25;12(0):144.
    Species: Human
    Sample Types: Serum
  44. Mechanisms of endothelial dysfunction in resistance arteries from patients with end-stage renal disease.
    Authors: Luksha L, Stenvinkel P, Hammarqvist F, Carrero JJ, Davidge ST, Kublickiene K
    PLoS ONE, 2012-04-26;7(4):e36056.
    Species: Human
    Sample Types: Serum
  45. Endogenous granulocyte colony-stimulating factor: a biomarker in acute ischemic stroke.
    Authors: Yu SC, Kuo CL, Huang CS, Chang CS, Wu SL, Su SL, Liu CS
    Biomarkers, 2012-03-23;17(4):319-24.
    Species: Human
    Sample Types: Plasma
  46. The type and quantity of dietary fat and carbohydrate alter faecal microbiome and short-chain fatty acid excretion in a metabolic syndrome 'at-risk' population.
    Authors: Fava, F, Gitau, R, Griffin, B A, Gibson, G R, Tuohy, K M, Lovegrove, J A
    Int J Obes (Lond), 2012-03-13;37(2):216-23.
    Species: Human
    Sample Types: Plasma
  47. Evaluation of endothelial function and subclinical atherosclerosis in association with hepatitis C virus in HIV-infected patients: a cross-sectional study.
    Authors: Masia M, Padilla S, Robledano C, Ramos J, Gutierrez F
    BMC Infect Dis, 2011-10-03;11(0):265.
    Species: Human
    Sample Types: Plasma
  48. Influence of a dose-dense adjuvant chemotherapy on sVCAM-1/sICAM-1 serum levels in breast cancer patients with 1-3 positive lymph nodes.
    Authors: Eggeman H, Stoblen F, Thill M, Korlach S, Schmid P, Luftner D, Elling D, Taran FA, Kummel S, Landt S
    Anticancer Res., 2011-08-01;31(8):2617-22.
    Species: Human
    Sample Types: Serum
  49. Prognostic significance of angiogenic factors in uterine cervical cancer.
    Authors: Landt S, Wehling M, Heidecke H, Jeschke S, Korlach S, Stoblen F, Schmid P, Blohmer JU, Lichtenegger W, Sehouli J, Kummel S
    Anticancer Res., 2011-08-01;31(8):2589-95.
    Species: Human
    Sample Types: Serum
  50. Serum levels of beta-catenin as a potential marker for genotype 4/hepatitis C-associated hepatocellular carcinoma.
    Authors: Zekri A, Bahnassy A, Alam El-Din H, Morsy H, Shaarawy S, Moharram N, Daoud S
    Oncol Rep, 2011-06-21;26(4):825-31.
    Species: Human
    Sample Types: Serum
  51. Plasma markers for identifying patients with metastatic melanoma.
    Authors: Kluger HM, Hoyt K, Bacchiocchi A, Mayer T, Kirsch J, Kluger Y, Sznol M, Ariyan S, Molinaro A, Halaban R
    Clin. Cancer Res., 2011-04-12;17(8):2417-25.
    Species: Human
    Sample Types: Plasma
  52. Angiopoietin-1 and angiopoietin-2 as clinically informative prognostic biomarkers of morbidity and mortality in severe sepsis*
    Authors: Ricciuto DR, Dos Santos CC, Hawkes M, Toltl LJ, Conroy AL, Rajwans N, Lafferty EI, Cook DJ, Fox-Robichaud A, Kahnamoui K, Kain KC, Liaw PC, Liles WC
    Crit. Care Med., 2011-04-01;0(0):.
    Species: Human
    Sample Types: Plasma
  53. Divergent anti-inflammatory effects of different oil acute consumption on healthy individuals.
    Authors: Papageorgiou N, Tousoulis D, Psaltopoulou T, Giolis A, Antoniades C, Tsiamis E, Miliou A, Toutouzas K, Siasos G, Stefanadis C
    Eur J Clin Nutr, 2011-02-16;65(4):514-9.
    Species: Human
    Sample Types: Serum
  54. Effects of dietary fat modification on insulin sensitivity and on other risk factors of the metabolic syndrome-LIPGENE: a European randomized dietary intervention study.
    Authors: Tierney AC, McMonagle J, Shaw DI, Gulseth HL, Helal O, Saris WH, Paniagua JA, Golabek-Leszczynska I, Defoort C, Williams CM, Karsltrom B, Vessby B, Dembinska-Kiec A, Lopez-Miranda J, Blaak EE, Drevon CA, Gibney MJ, Lovegrove JA, Roche HM
    Int J Obes (Lond), 2010-10-12;35(6):800-9.
    Species: Human
    Sample Types: Serum
  55. Matrix metalloproteinase-9 and plasminogen activator inhibitor-1 are associated with right ventricular structure and function: the MESA-RV Study.
    Authors: Kawut SM, Barr RG, Johnson WC
    Biomarkers, 2010-10-05;15(8):731-8.
    Species: Human
    Sample Types: Plasma
  56. Plasma protein growth arrest-specific 6 levels are associated with altered glucose tolerance, inflammation, and endothelial dysfunction.
    Authors: Hung YJ, Lee CH, Chu NF
    Diabetes Care, 2010-05-26;33(8):1840-4.
    Species: Human
    Sample Types: Plasma
  57. Effect of intensive lifestyle changes on endothelial function and on inflammatory markers of atherosclerosis.
    Authors: Dod HS, Bhardwaj R, Sajja V, Weidner G, Hobbs GR, Konat GW, Manivannan S, Gharib W, Warden BE, Nanda NC, Beto RJ, Ornish D, Jain AC
    Am. J. Cardiol., 2010-02-01;105(3):362-7.
    Species: Human
    Sample Types: Serum
  58. SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity.
    Authors: Rutanen J, Yaluri N, Modi S, Pihlajamaki J, Vanttinen M, Itkonen P, Kainulainen S, Yamamoto H, Lagouge M, Sinclair DA, Elliott P, Westphal C, Auwerx J, Laakso M
    Diabetes, 2010-01-27;59(4):829-35.
    Species: Human
    Sample Types: Serum
  59. Prehospital resuscitation with hypertonic saline-dextran modulates inflammatory, coagulation and endothelial activation marker profiles in severe traumatic brain injured patients.
    Authors: Rhind SG, Crnko NT, Baker AJ
    J Neuroinflammation, 2010-01-18;7(0):5.
    Species: Human
    Sample Types: Serum
  60. Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
    Authors: Ruotsalainen E, Stancakova A, Vauhkonen I, Salmenniemi U, Pihlajamaki J, Punnonen K, Laakso M
    Atherosclerosis, 2009-12-02;210(2):536-41.
    Species: Human
    Sample Types: Plasma
  61. Sensitive plasma protein analysis by microparticle-based proximity ligation assays.
    Authors: Darmanis S, Nong RY, Hammond M, Gu J, Alderborn A, Vanelid J, Siegbahn A, Gustafsdottir S, Ericsson O, Landegren U, Kamali-Moghaddam M
    Mol. Cell Proteomics, 2009-11-27;9(2):327-35.
    Species: Human
    Sample Types: Plasma
  62. Intensive insulin therapy decreases urinary MCP-1 and ICAM-1 excretions in incipient diabetic nephropathy.
    Authors: Ye SD, Zheng M, Zhao LL, Qian Y, Yao XM, Ren A, Li SM, Jing CY
    Eur. J. Clin. Invest., 2009-08-03;39(11):980-5.
    Species: Human
    Sample Types: Urine
  63. Immuno-inflammatory predictors of stroke at follow-up in patients with chronic non-valvular atrial fibrillation (NVAF).
    Authors: Pinto A, Tuttolomondo A, Casuccio A, Di Raimondo D, Di Sciacca R, Arnao V, Licata G
    Clin. Sci., 2009-05-01;116(10):781-9.
    Species: Human
    Sample Types: Plasma
  64. Non-conventional markers of atherosclerosis before and after gastric banding surgery.
    Authors: Hanusch-Enserer U, Zorn G, Wojta J, Kopp CW, Prager R, Koenig W, Schillinger M, Roden M, Huber K
    Eur. Heart J., 2009-04-20;30(12):1516-24.
    Species: Human
    Sample Types: Plasma
  65. Macrophage inflammatory protein-1beta induced cell adhesion with increased intracellular reactive oxygen species.
    Authors: Tatara Y, Ohishi M, Yamamoto K, Shiota A, Hayashi N, Iwamoto Y, Takeda M, Takagi T, Katsuya T, Ogihara T, Rakugi H
    J. Mol. Cell. Cardiol., 2009-03-26;47(1):104-11.
    Species: Human
    Sample Types: Plasma
  66. Angiopoietin-2 is associated with decreased endothelial nitric oxide and poor clinical outcome in severe falciparum malaria.
    Authors: Yeo TW, Lampah DA, Gitawati R, Tjitra E, Kenangalem E, Piera K, Price RN, Duffull SB, Celermajer DS, Anstey NM
    Proc. Natl. Acad. Sci. U.S.A., 2008-10-28;105(44):17097-102.
    Species: Human
    Sample Types: Plasma
  67. Association of soluble cell adhesion molecules with ankle-brachial index in a biethnic cohort of predominantly hypertensive individuals.
    Authors: Khaleghi M, Ali Z, Mosley TH, Turner ST, Kullo IJ
    Clin. Chem., 2008-09-11;54(11):1788-95.
    Species: Human
    Sample Types: Plasma
  68. Cross-sectional relations of multiple inflammatory biomarkers to peripheral arterial disease: The Framingham Offspring Study.
    Authors: Murabito JM, Keyes MJ, Guo CY, Keaney JF, Vasan RS, D'Agostino RB, Benjamin EJ
    Atherosclerosis, 2008-07-09;203(2):509-14.
    Species: Human
    Sample Types: Plasma
  69. Immuno-inflammatory and thrombotic/fibrinolytic variables associated with acute ischemic stroke diagnosis.
    Authors: Tuttolomondo A, Pinto A, Corrao S, Di Raimondo D, Fernandez P, Di Sciacca R, Arnao V, Licata G
    Atherosclerosis, 2008-07-09;203(2):503-8.
    Species: Human
    Sample Types: Plasma
  70. Effects of darbepoetin-alpha on plasma pro-inflammatory cytokines, anti-inflammatory cytokine interleukin-10 and soluble Fas/Fas ligand system in anemic patients with chronic heart failure.
    Authors: Kourea K, Parissis JT, Farmakis D, Panou F, Paraskevaidis I, Venetsanou K, Filippatos G, Kremastinos DT
    Atherosclerosis, 2007-11-07;199(1):215-21.
    Species: Human
    Sample Types: Plasma
  71. Consumption of isoflavone-rich soy protein does not alter homocysteine or markers of inflammation in postmenopausal women.
    Authors: Greany KA, Nettleton JA, Wangen KE, Thomas W, Kurzer MS
    Eur J Clin Nutr, 2007-09-05;62(0):1419-25.
    Species: Human
    Sample Types: Serum
  72. Rosiglitazone produces a greater reduction in circulating platelet activity compared with gliclazide in patients with type 2 diabetes mellitus--an effect probably mediated by direct platelet PPARgamma activation.
    Authors: Khanolkar MP, Morris RH, Thomas AW, Bolusani H, Roberts AW, Geen J, Jackson SK, Evans LM
    Atherosclerosis, 2007-08-31;197(2):718-24.
    Species: Human
    Sample Types: Serum

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Reviews for Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit

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Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
By Anonymous on 06/27/2023
Sample Tested: EDTA Plasma
Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit DCD540
Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
By Steven Edmund on 12/06/2021
Sample Tested: Serum
Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit DCD540
Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
By Anonymous on 10/21/2021
Sample Tested: Serum and Plasma

Incredibly easy method and good results

Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit DCD540
Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
By Anonymous on 10/14/2021
Sample Tested: Serum

Easy to use, quick assay, good results

Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit DCD540
Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
By Anonymous on 02/04/2021
Sample Tested: Plasma

Curve developed beautifully, with clear instructions and QC reflecting the sample type.

Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit DCD540
Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
By Anonymous on 06/08/2020
Sample Tested: Serum-free Cell Culture Supernates
Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
By Anonymous on 02/15/2020
Sample Tested: Colon cancer cell line
Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit DCD540
Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
By Anonymous on 01/13/2020
Sample Tested: Serum-free Cell Culture Media
Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
By Anonymous on 12/08/2019
Sample Tested: Cell culture supernatant
Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit
By Anonymous on 09/07/2017
Sample Tested: HPMEC