Human CCL4/MIP-1 beta Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 23.3 | 98.8 | 428 | 31.4 | 118 | 476 |
Standard Deviation | 1.1 | 3.8 | 17.3 | 3.7 | 9.9 | 28 |
CV% | 4.7 | 3.8 | 4 | 11.8 | 8.4 | 5.9 |
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 51.3 | 208 | 932 | 58.8 | 220 | 949 |
Standard Deviation | 4.6 | 7.5 | 30.2 | 5.7 | 15.6 | 70.5 |
CV% | 9 | 3.6 | 3.2 | 9.7 | 7.1 | 7.4 |
Recovery
The recovery of MIP-1 beta spiked to three levels in samples throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=5) | 107 | 81-117 |
Citrate Plasma (n=5) | 99 | 87-116 |
EDTA Plasma (n=5) | 100 | 84-112 |
Heparin Plasma (n=5) | 99 | 90-112 |
Serum (n=5) | 99 | 88-113 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: CCL4/MIP-1 beta
CCL4/MIP-1 beta is a beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. It attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha. It signals through CCR5, and an N-terminally trimmed form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- For Serum & Plasma Samples: Add 100 µL of Assay Diluent to each well.
For Cell Culture Supernate Samples: Add 50 µL Assay Diluent to each well. - For Serum & Plasma Samples: Add 150 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
For Cell Culture Supernate Samples: Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1.25 hours. - Aspirate each well and wash, repeating the process twice for a total of 3 washes.
- Add 200 µL of Conjugate to each well.
- For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1.25 hours. - Aspirate and wash 3 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human CCL4/MIP-1 beta Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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A Comparative Analysis of Innate Immune Responses and the Structural Characterization of Spike from SARS-CoV-2 Gamma Variants and Subvariants
Authors: Scovino, AM;Dahab, EC;Diniz-Lima, I;de Senna Silveira, E;Barroso, SPC;Cardoso, KM;Nico, D;Makhoul, GJ;da Silva-Junior, EB;Freire-de-Lima, CG;Freire-de-Lima, L;Fonseca, LMD;Valente, N;Nacife, V;Machado, A;Araújo, M;Vieira, GF;Pauvolid-Corrêa, A;Siqueira, M;Morrot, A;
Microorganisms
Species: Human
Sample Types: Cell Culture Supernates
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TCR-mimic bispecific antibodies to target the HIV-1 reservoir
Authors: S Sengupta, NL Board, F Wu, M Moskovljev, J Douglass, J Zhang, BR Reinhold, J Duke-Cohan, J Yu, MC Reed, Y Tabdili, A Azurmendi, EJ Fray, H Zhang, EH Hsiue, K Jenike, YC Ho, SB Gabelli, KW Kinzler, B Vogelstein, S Zhou, JD Siliciano, S Sadegh-Nas, EL Reinherz, RF Siliciano
Proceedings of the National Academy of Sciences of the United States of America, 2022-04-08;119(15):e2123406119.
Species: Human
Sample Types: Cell culture supernate
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Distinct BTK inhibitors differentially induce apoptosis but similarly suppress chemotaxis and lipid accumulation in mantle cell lymphoma
Authors: Z Liu, J Liu, T Zhang, L Li, S Zhang, H Jia, Y Xia, M Shi, J Zhang, S Yue, X Chen, J Yu
BMC Cancer, 2021-06-26;21(1):732.
Species: Human
Sample Types: Cell Culture Supernates
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Cytokine expression patterns in hospitalized children with Bordetella pertussis, Rhinovirus or co-infection
Authors: E Pandolfi, N Panera, A Alisi, E Carloni, L Russo, I Campagna, C Rizzo, C Concato, G Linardos, L Piccioni, S Jackson, A Villani, F Midulla, AE Tozzi
Scientific Reports, 2021-05-26;11(1):10948.
Species: Human
Sample Types: Sputum
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CXCL13 plasma levels function as a biomarker for disease activity in patients with chronic lymphocytic leukemia
Authors: M Sivina, L Xiao, E Kim, A Vaca, SS Chen, MJ Keating, A Ferrajoli, Z Estrov, N Jain, WG Wierda, X Huang, N Chiorazzi, JA Burger
Leukemia, 2020-10-21;0(0):.
Species: Human
Sample Types: Plasma
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TNF-alpha and IL-1beta sensitize human MSC for IFN-gamma signalling and enhance neutrophil recruitment
Authors: A Hackel, A Aksamit, K Bruderek, S Lang, S Brandau
Eur. J. Immunol., 2020-09-24;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Cognitive functional therapy (CFT) compared with core training exercise (CTE) in patients with failed back surgery syndrome (FBSS): A study protocol for a randomized controlled trial
Authors: L Avila, ML Neves, AR Abreu, CR Fiuza, L Fukusawa, N Meziat-Fil, AR Soares San
Journal of bodywork and movement therapies, 2020-09-14;26(0):428-434.
Species: Human
Sample Types: Serum
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Differential B-Cell Receptor Signaling Requirement for Adhesion of Mantle Cell Lymphoma Cells to Stromal Cells
Authors: L Sadeghi, G Arvidsson, M Merrien, A M Wasik, A Görgens, CIE Smith, B Sander, AP Wright
Cancers (Basel), 2020-05-02;12(5):.
Species: Human
Sample Types: Cell Culture Supernates
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4D electron microscopy of T cell activation
Authors: Y Lu, BK Yoo, AHC Ng, J Kim, S Yeom, J Tang, MM Lin, AH Zewail, JR Heath
Proc. Natl. Acad. Sci. U.S.A., 2019-10-14;116(44):22014-22019.
Species: Human
Sample Types: Cell Culture Supernates
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Biological variation of immunological blood biomarkers in healthy individuals and quality goals for biomarker tests
Authors: N Aziz, R Detels, JJ Quint, D Gjertson, T Ryner, AW Butch
BMC Immunol., 2019-09-14;20(1):33.
Species: Human
Sample Types: Serum
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Vascular Adhesion Protein-1 (VAP-1) as Predictor of Radiographic Severity in Symptomatic Knee Osteoarthritis in the New York University Cohort
Authors: E Bournazou, J Samuels, H Zhou, S Krasnokuts, J Patel, T Han, J Bencardino, L Rybak, SB Abramson, U Junker, KS Brown, M Attur
Int J Mol Sci, 2019-05-29;20(11):.
Species: Human
Sample Types: Synovial Fluid
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A novel orally available Syk/Src/Jak2 inhibitor, SKLB-850, showed potent anti-tumor activities in B cell lymphoma (BCL) models
Authors: N Zhang, G Zhang, N Liu, W Lin, S Ji, M Zheng, K Chen, X Liang, G Li, Y Ma, J Zhu, T Niu, LL Li, J Li, YQ Wei, SY Yang
Oncotarget, 2017-12-01;8(67):111495-111507.
Species: Human
Sample Types: Cell Culture Supernates
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Profiling of cytokines, chemokines and other soluble proteins as a potential biomarker in colorectal cancer and polyps
Authors: NA Johdi, L Mazlan, I Sagap, R Jamal
Cytokine, 2017-07-06;99(0):35-42.
Species: Human
Sample Types: Serum
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Functional Differences between IgM and IgD Signaling in Chronic Lymphocytic Leukemia
J Immunol, 2016-08-17;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Stability of cytokines, chemokines and soluble activation markers in unprocessed blood stored under different conditions
Cytokine, 2016-05-18;84(0):17-24.
Species: Human
Sample Types: Serum
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Serum levels of chemokines CCL4 and CCL5 in cirrhotic patients indicate the presence of hepatocellular carcinoma.
Authors: Sadeghi M, Lahdou I, Oweira H, Daniel V, Terness P, Schmidt J, Weiss K, Longerich T, Schemmer P, Opelz G, Mehrabi A
Br J Cancer, 2015-08-13;113(5):756-62.
Species: Human
Sample Types: Serum
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The phosphoinositide-3-kinase (PI3K)-delta and gamma inhibitor, IPI-145 (Duvelisib), overcomes signals from the PI3K/AKT/S6 pathway and promotes apoptosis in CLL.
Authors: Balakrishnan K, Peluso M, Fu M, Rosin N, Burger J, Wierda W, Keating M, Faia K, O'Brien S, Kutok J, Gandhi V
Leukemia, 2015-04-28;29(9):1811-22.
Species: Human
Sample Types: Cell Culture Supernates
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Pleurocidin, a novel antimicrobial peptide, induces human mast cell activation through the FPRL1 receptor.
Authors: Pundir P, Catalli A, Leggiadro C, Douglas S, Kulka M
Mucosal Immunol, 2013-07-10;7(1):177-87.
Species: Human
Sample Types: Cell Culture Supernates
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Regulation of inflammatory chemokine receptors on blood T cells associated to the circulating versus liver chemokines in dengue Fever.
Authors: de-Oliveira-Pinto LM, Marinho CF, Povoa TF, de Azeredo EL, de Souza LA, Barbosa LD, Motta-Castro AR, Alves AM, Avila CA, de Souza LJ, da Cunha RV, Damasco PV, Paes MV, Kubelka CF
PLoS ONE, 2012-07-16;7(7):e38527.
Species: Human
Sample Types: Plasma
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The Bruton tyrosine kinase inhibitor PCI-32765 thwarts chronic lymphocytic leukemia cell survival and tissue homing in vitro and in vivo.
Authors: Ponader S, Chen SS, Buggy JJ
Blood, 2011-12-16;119(5):1182-9.
Species: Human
Sample Types: Plasma
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Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2.
Authors: Johnson TR, McLellan JS, Graham BS
J. Virol., 2011-11-16;86(3):1339-47.
Species: Human
Sample Types: Cell Culture Supernates
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Lenalidomide as initial therapy of elderly patients with chronic lymphocytic leukemia.
Authors: Badoux XC, Keating MJ, Wen S
Blood, 2011-07-01;118(13):3489-98.
Species: Human
Sample Types: Plasma
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CCL3 (MIP-1alpha) plasma levels and the risk for disease progression in chronic lymphocytic leukemia.
Authors: Sivina M, Hartmann E, Kipps TJ, Rassenti L, Krupnik D, Lerner S, LaPushin R, Xiao L, Huang X, Werner L, Neuberg D, Kantarjian H, O'Brien S, Wierda WG, Keating MJ, Rosenwald A, Burger JA
Blood, 2010-11-29;117(5):1662-9.
Species: Human
Sample Types: Plasma
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Macrophage inflammatory protein-1beta induced cell adhesion with increased intracellular reactive oxygen species.
Authors: Tatara Y, Ohishi M, Yamamoto K, Shiota A, Hayashi N, Iwamoto Y, Takeda M, Takagi T, Katsuya T, Ogihara T, Rakugi H
J. Mol. Cell. Cardiol., 2009-03-26;47(1):104-11.
Species: Human
Sample Types: Plasma
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Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
J. Immunol., 2008-12-01;181(11):8053-67.
Species: Human
Sample Types: BALF
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NF-kappaB modulates sensitivity to apoptosis, proinflammatory and migratory potential in short- versus long-term cultured human gammadelta lymphocytes.
Authors: Ferrarini M, Delfanti F, Gianolini M, Rizzi C, Alfano M, Lazzarin A, Biswas P
J. Immunol., 2008-11-01;181(9):5857-64.
Species: Human
Sample Types: Cell Culture Supernates
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In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22.
Authors: Olsnes AM, Ryningen A, Ersvaer E, Bruserud Ø
J. Interferon Cytokine Res., 2008-05-01;28(5):297-310.
Species: Human
Sample Types: Cell Culture Supernates
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Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions.
Authors: Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A
Int. J. Cancer, 2008-02-15;122(4):769-76.
Species: Human
Sample Types: Cell Culture Supernates
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Duffy antigen modifies the chemokine response in human endotoxemia.
Authors: Mayr FB, Spiel AO, Leitner JM, Firbas C, Kliegel T, Jilma-Stohlawetz P, Derendorf H, Jilma B
Crit. Care Med., 2008-01-01;36(1):159-65.
Species: Human
Sample Types: Plasma
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Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction.
Authors: Bolovan-Fritts CA, Spector SA
Blood, 2007-09-25;111(1):175-82.
Species: Human
Sample Types: Cell Culture Supernates
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High T-cell response to human cytomegalovirus induces chemokine-mediated endothelial cell damage.
Authors: Bolovan-Fritts CA, Trout RN, Spector SA
Blood, 2007-05-22;110(6):1857-63.
Species: Human
Sample Types: Cell Culture Supernates
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Human Vgamma2Vdelta2 T cells contain cytoplasmic RANTES.
Authors: Tikhonov I, Deetz CO, Paca R, Berg S, Lukyanenko V, Lim JK, Pauza CD
Int. Immunol., 2006-06-01;18(8):1243-51.
Species: Human
Sample Types: Cell Culture Supernates
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A network-based analysis of the late-phase reaction of the skin.
Authors: Benson M, Langston MA, Adner M, Andersson B, Torinssson-Naluai A, Cardell LO
J. Allergy Clin. Immunol., 2006-05-11;118(1):220-5.
Species: Human
Sample Types: Cell Culture Supernates
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Respiratory syncytial virus and other respiratory viruses during the first 3 months of life promote a local TH2-like response.
Authors: Kristjansson S, Bjarnarson SP, Wennergren G, Palsdottir AH, Arnadottir T, Haraldsson A, Jonsdottir I
J. Allergy Clin. Immunol., 2005-10-01;116(4):805-11.
Species: Human
Sample Types: Tissue Secretion
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CCR5 expression and CC chemokine levels in idiopathic pulmonary fibrosis.
Authors: Capelli A, Di Stefano A, Gnemmi I, Donner CF
Eur. Respir. J., 2005-04-01;25(4):701-7.
Species: Human
Sample Types: BALF
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Concentrations of macrophage inflammatory proteins MIP-1alpha and MIP-1beta and interleukin 8 (il-8) in lyme borreliosis.
Authors: Grygorczuk S, Pancewicz S, Zajkowska J, Kondrusik M, Rwierzbinska R, Hermanowska-Szpakowicz T
Infection, 2004-12-01;32(6):350-5.
Species: Human
Sample Types: CSF
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Inhibition of human immunodeficiency virus type 1 replication by Z-100, an immunomodulator extracted from human-type tubercle bacilli, in macrophages.
Authors: Emori Y, Ikeda T, Ohashi T, Masuda T, Kurimoto T, Takei M, Kannagi M
J. Gen. Virol., 2004-09-01;85(0):2603-13.
Species: Human
Sample Types: Cell Culture Supernates
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Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells.
Authors: Megjugorac NJ, Young HA, Amrute SB, Olshalsky SL, Fitzgerald-Bocarsly P
J. Leukoc. Biol., 2004-01-23;75(3):504-14.
Species: Human
Sample Types: Cell Culture Supernates
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Impact of NNRTI compared to PI-based highly active antiretroviral therapy on CCR5 receptor expression, beta-chemokines and IL-16 secretion in HIV-1 infection.
Authors: Burton CT, »V6þÌ™+ìZ, Hardy GA, Sullivan AK, Nelson MR, Gazzard B, Gotch FM, Imami N
2002-11-01;130(2):286-92.
Species: Human
Sample Types: Plasma
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Endogenously expressed nef uncouples cytokine and chemokine production from membrane phenotypic maturation in dendritic cells.
Authors: Messmer D, Jacque JM, Santisteban C, Bristow C, Han SY, Villamide-Herrera L, Mehlhop E, Marx PA, Steinman RM, Gettie A, Pope M
J. Immunol., 2002-10-15;169(8):4172-82.
Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Cell Culture Supernates
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Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes.
Authors: D'Ambrosio D
Eur. J. Immunol., 2002-10-01;32(10):2934-43.
Species: Human
Sample Types: Cell Culture Supernates
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CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor.
Authors: Honczarenko M, Le Y, Glodek AM, Silberstein LE
Blood, 2002-10-01;100(7):2321-9.
Species: Human
Sample Types: Cell Culture Supernates
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TNFalpha-induced macrophage chemokine secretion is more dependent on NF-kappaB expression than lipopolysaccharides-induced macrophage chemokine secretion.
Authors: Ciesielski CJ, Andreakos E, Foxwell BM, Feldmann M
Eur. J. Immunol., 2002-07-01;32(7):2037-45.
Species: Human
Sample Types: Cell Culture Supernates
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Analysis of chemokines and chemokine receptor expression in ovarian cancer ascites.
Authors: Milliken D, Scotton C, Raju S, Balkwill F, Wilson J
Clin. Cancer Res., 2002-04-01;8(4):1108-14.
Species: Human
Sample Types: Plasma
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Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes.
Authors: Binninger-Schinzel D, 2019, Norley S, Adler HS, Oberg HH, Kurth R
2002-03-01;294(1):209-21.
Species: Human
Sample Types: Cell Culture Supernates
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Amyloid beta and amylin fibrils induce increases in proinflammatory cytokine and chemokine production by THP-1 cells and murine microglia.
Authors: Yates SL, Burgess LH, Kocsis-Angle J, Antal JM, Dority MD, Embury PB, Piotrkowski AM, Brunden KR
J. Neurochem., 2000-03-01;74(3):1017-25.
Species: Human
Sample Types: Cell Culture Supernates
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Immunization with live attenuated simian immunodeficiency virus induces strong type 1 T helper responses and beta-chemokine production.
Authors: Gauduin MC, Glickman RL, Ahmad S, Yilma T, Johnson RP
Proc. Natl. Acad. Sci. U.S.A., 1999-11-23;96(24):14031-6.
Species: Primate
Sample Types: Cell Culture Supernates
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Interleukin-12 induces sustained activation of multiple host inflammatory mediator systems in chimpanzees.
Authors: Lauw FN, Dekkers PE, te Velde AA, Speelman P, Levi M, Kurimoto M, Hack CE, Van Deventer SJ, van der Poll T
J. Infect. Dis., 1999-03-01;179(3):646-52.
Species: Primate - Pan troglodytes (Chimpanzee)
Sample Types: Plasma
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Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques.
Authors: Lehner T, —îÊëÖ“§, Wang Y, r¹oŽ&A½ýx, Cranage M, Ïï§Ù´âoÑ‘, Bergmeier LA, Mitchell E, Tao L, Hall G, Dennis M, Cook N, Brookes R, Klavinskis L, Jones I, Doyle C, Ward R
1996-07-01;2(7):767-75.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Cell Culture Supernates
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