Many Proteome Profiler™ arrays are amenable to near-infrared (NIR) fluorescence detection using the LI-COR Odyssey® Infrared Imaging System. To achieve this adaption, the HRP-conjugated Streptavidin provided in the kit is replaced with IRDye® 800CW Streptavidin, and the arrays are scanned using a LI-COR Odyssey Infrared Imaging System. This protocol is not applicable to all Proteome Profiler™ array kits. It is not applicable to arrays that use an HRP-conjugated detection antibody instead of Streptavidin-HRP, and is not recommended for arrays with phospho-specific antibodies due to increased signal noise. Representative data using LI-COR detection are shown for the Proteome Profiler™ array kits that have been tested. Proteome Profiler™ array kits that have not been tested may or may not be applicable to the LI-COR Odyssey® Infrared Imaging System, or may require optimization by the customer.
Use of Proteome Profiler™ Arrays with LI-COR Detection
- LI-COR Data Examples for Human Adipokine Array Kit
- LI-COR Data Examples for Human Angiogenesis Array Kit
- LI-COR Data Examples for Human Cytokine Array Panel A
- LI-COR Data Examples for Human XL Cytokine Array Kit
- LI-COR Data Examples for Mouse Cytokine Array Panel A
- LI-COR Data Examples for Mouse XL Cytokine Array
- LI-COR Data Examples for Rat Cytokine Array Kit Panel A
- LI-COR Data Examples for Human Kidney Biomarker Array Kit
- LI-COR Data Examples for the Human Oncology Array Kit
- Use of Proteome Profiler Arrays with LI-COR Detection