LI-COR Data Examples for Human XL Cytokine Array Kit

Data Examples

Profiling Proteins in Cell Culture Supernates

Figure 1. The Human XL Cytokine Array detects multiple analytes in cell culture supernates. HUVEC human umbilical vein endothelial cells were untreated or treated with 100 μg/mL recombinant human TNF-alpha (R&D Systems, Catalog # 210-TA) for 24 hours In each array shown, 500 µL of cell supernate was used. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

Profiling Proteins in Cell Culture Lysates

Figure 2. The Human XL Cytokine Array detects multiple analytes in cell culture lysates. A431 human epithelial carcinoma cells were either left untreated or treated with 50 ng/mL of recombinant human EGF (R&D Systems, Catalog # 236-EG) for 24 hours. 200 μg of cell lysate was run on each array. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

Profiling Proteins in Tissue Lysate, PBMC Supernates & Body Fluids

1 C-Reactive Protein
2 FGF basic
3 DPPIV
4 Lipocalin-2
5 Resistin
6 Chitinase 3-like 1
7 GRO-alpha
8 IL-8
9 IL-1alpha
10 Adiponectin
11 ICAM-1
12 IGFBP-2
13 RBP-4
14 Angiogenin
15 Complement Factor D
16 IL-19
17 Vitamin D BP
18 EGF
19 GDF-15
20 IL-18 BpA
21 Kallirein 3/PSA
22 Cystatin C
23 IL-1ra
24 MMP-9
25 Myeloperoxidase
26 Osteopontin

Figure 3. The Human XL Cytokine Array detects multiple analytes in tissue lysates, PBMC supernates, serum, milk, urine and saliva samples.

The sample type and quantity used per array is listed. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

Odyssey is registered trademarks of LI-COR, Inc.