Bovine FGF basic/FGF2/bFGF Protein Summary
Product Specifications
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
133-FB
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
133-FB/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: FGF basic/FGF2/bFGF
FGF basic is a member of the FGF family, currently comprised of seven related mitogenic proteins which show 35 - 55% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum and placenta. This factor contains four cysteine residues but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. Several reports indicate that a variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions apparently affect localization of FGF basic in cellular compartments but do not affect biological activity. Studies indicate that binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities.
FGF basic stimulates the proliferation of all cells of mesodermal origin, and many cells of neuroectodermal, ectodermal and endodermal origin. The cells include fibroblasts, endothelial cells, astrocytes, oligodendrocytes, neuroblasts, keratinocytes, osteoblasts, smooth muscle cells, and melanocytes. FGF basic is chemotactic and mitogenic for endothelial cells in vitro. FGF basic induces neuron differentiation, survival and regeneration. FGF basic has also been shown to be crucial in modulating embryonic development and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.
Citations for Bovine FGF basic/FGF2/bFGF Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Macrophages modulate fibrosis during newt lens regeneration
Authors: Tsissios, G;Sallese, A;Raul Perez-Estrada, J;Tangeman, JA;Chen, W;Smucker, B;Ratvasky, SC;Grajales-Esquivel, E;Martinez, A;Visser, KJ;Araus, AJ;Wang, H;Simon, A;Yun, MH;Del Rio-Tsonis, K;
bioRxiv : the preprint server for biology
Species: Newt
Sample Types: In Vivo
Applications: In Vivo -
A Stage-Specific OTX2 Regulatory Network and Maturation-Associated Gene Programs Are Inherent Barriers to RPE Neural Competency.
Authors: Tangeman J, Perez-Estrada J, Van Zeeland E, Liu L, Danciutiu A, Grajales-Esquivel E, Smucker B, Liang C, Del Rio-Tsonis K
Front Cell Dev Biol, 2022-04-19;10(0):875155.
Species: Chicken
Sample Types: In Vivo
Applications: In Vivo -
Hic1 Defines Quiescent Mesenchymal Progenitor Subpopulations with Distinct Functions and Fates in Skeletal Muscle Regeneration
Authors: RW Scott, M Arostegui, R Schweitzer, FMV Rossi, TM Underhill
Cell Stem Cell, 2019-12-05;25(6):797-813.e9.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Transcriptional repressor GATA binding 1-mediated repression of SRY-box 2 expression suppresses cancer stem cell functions and tumor initiation
Authors: X Gong, W Liu, L Wu, Z Ma, Y Wang, S Yu, J Zhang, H Xie, G Wei, F Ma, L Lu, L Chen
J. Biol. Chem., 2018-10-12;0(0):.
Species: Human
Sample Types: Transfected Whole Cells
Applications: Bioassay -
Dual function of TGF? in lens epithelial cell fate: implications for secondary cataract
Authors: BA Boswell, A Korol, JA West-Mays, LS Musil
Mol. Biol. Cell, 2017-02-16;0(0):.
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay -
Single transcription factor reprogramming of hair follicle dermal papilla cells to induced pluripotent stem cells.
Authors: Tsai SY, Bouwman BA, Ang YS, Kim SJ, Lee DF, Lemischka IR, Rendl M
Stem Cells, 2011-06-01;29(6):964-71.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Essential role of BMPs in FGF-induced secondary lens fiber differentiation.
Authors: Boswell BA, Overbeek PA, Musil LS
Dev. Biol., 2008-09-18;324(2):202-12.
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay -
Activation of multiple signaling pathways is critical for fibroblast growth factor 2- and vascular endothelial growth factor-stimulated ovine fetoplacental endothelial cell proliferation.
Authors: Zheng J, Wen Y, Song Y, Wang K, Chen DB, Magness RR
Biol. Reprod., 2007-09-26;78(1):143-50.
Species: Ovine
Sample Types: Whole Cells
Applications: Bioassay -
Carcinoembryonic antigen-related cell adhesion molecule 1 modulates vascular remodeling in vitro and in vivo.
Authors: Horst AK, Ito WD, Dabelstein J, Schumacher U, Sander H, Turbide C, Brummer J, Meinertz T, Beauchemin N, Wagener C
J. Clin. Invest., 2006-05-04;116(6):1596-605.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Effect of cytokine treatment on the neurogenesis process in the brain of soman-poisoned mice.
Authors: Collombet JM, Four E, Burckhart MF, Masqueliez C, Bernabe D, Baubichon D, Herodin F, Lallement G
Toxicology, 2005-05-15;210(1):9-23.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
FAQs
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What receptors does FGF basic bind?
FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.rndsystems.com/resources/articles/fibroblast-growth-factors-and-their-receptors
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