Canine CD34 Antibody

Catalog #: MAB3346
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3346
MAB3346-SP

Detection of CD34 in CLL‑1390 Canine Cell Line by Flow Cytometry.

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All CD34 Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Canine CD34 Antibody Summary

Species Reactivity

Canine

Specificity

Detects canine CD34 in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 1H6

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant canine CD34

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

McSweeney, P.A. et al. (1998) Blood 91(6):1977. This application was not tested by R&D Systems.

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD34 in CLL‑1390 Canine Cell Line by Flow Cytometry. CLL-1390 canine leukocytic round cell neoplasia cell line was stained with Mouse Anti-Canine CD34 Monoclonal Antibody (Catalog # MAB3346, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD34

CD34 is a sialomucin molecule that is expressed on primitive hematopoietic stem cells and downregulated as they differentiate into mature cells. Although its precise function remains unknown, the pattern of expression of CD34 suggests that it plays a significant role in early hematopoiesis.

Entrez Gene IDs

947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)

Alternate Names

CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Canine CD34 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Comparison of Properties of Stem Cells Isolated from Adipose Tissue and Lipomas in Dogs
Authors: T Teshima, A Matsuoka, M Shiba, K Dairaku, H Matsumoto, R Suzuki, H Koyama
Stem Cells Int, 2019-11-20;2019(0):1609876.
Species: Canine
Sample Types: Whole Cells
Applications: Flow Cytometry
Acellular Endocardium as a Novel Biomaterial for the Intima of Tissue-Engineered Small-Caliber Vascular Grafts
Authors: Feng Wang
Artif Organs, 2016-12-01;40(12):E253-E265.
Species: Canine
Sample Types: Whole Cells
Applications: Functional Assay
Characterization of monoclonal antibodies that recognize canine CD34.
Authors: McSweeney PA, Rouleau KA, Wallace PM, Bruno B, Andrews RG, Krizanac-Bengez L, Sandmaier BM, Storb R, Wayner E, Nash RA
Blood, 1998-03-15;91(6):1977-86.
Species: Canine
Sample Types: Recombinant Protein, Whole Cells
Applications: Flow Cytometry, Surface Plasmon Resonance, Western Blot