Home / CD34 / Canine CD34 Fluorescein-conjugated Antibody

Canine CD34 Fluorescein-conjugated Antibody

Catalog #: FAB3346F
1 Citation Datasheet / COA / SDS

Discontinued Product

FAB3346F has been discontinued.
View all CD34 products.
Detection of CD34 in CLL‑1390 Canine Cell Line by Flow Cytometry.
1 Image
Product Details
Citations (1)
FAQs
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Canine CD34 Fluorescein-conjugated Antibody Summary

Species Reactivity
Canine
Specificity
Detects canine CD34 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 1H6
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant canine CD34
Accession # Q28270
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD34 antibody in CLL-1390 Canine Cell Line antibody by Flow Cytometry. View Larger

Detection of CD34 in CLL‑1390 Canine Cell Line by Flow Cytometry. CLL-1390 canine leukocytic round cell neoplasia cell line was stained with Mouse Anti-Canine CD34 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3346F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD34

CD34 is a 100-125 kDa highly glycosylated member of the CD34 family of molecules. In canine, mature CD34 is a 356 amino acid (aa) type I transmembrane protein that contains a 261 aa extracellular domain (ECD). Although originally identified on the surface of hematopoietic stem cells and vascular endothelial progenitors, it is now known to be expressed by highly divergent cell types such as telocytes/ICLC (cardiac support cells), cytotoxic NK lineage restricted progenitors, muscle satellite cells, corneal keratocytes (fibroblasts) and dendritic cell progenitors (with the exception of pre-conventional DCs). Little is known about CD34 ligands, save for L-Selectin. Canine CD34 ECD shares 60% and 56% aa sequence identity with human and mouse CD34 ECD, respectively.

Entrez Gene IDs
947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
Alternate Names
CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Canine CD34 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Allogeneic multipotent mesenchymal stromal cell sheet transplantation promotes healthy healing of wounds caused by zoledronate and dexamethasone in canine mandibular bones
    Authors: N Kaibuchi, T Iwata, S Onizuka, K Yano, Y Tsumanuma, M Yamato, T Okano, T Ando
    Regen Ther, 2019-01-12;10(0):77-83.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Canine CD34 Fluorescein-conjugated Antibody

There are currently no reviews for this product. Be the first to review Canine CD34 Fluorescein-conjugated Antibody and earn rewards!

Have you used Canine CD34 Fluorescein-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review