Home / IL-1 beta/IL-1F2 / Canine IL-1 beta/IL-1F2 DuoSet ELISA

Canine IL-1 beta/IL-1F2 DuoSet ELISA

Catalog #: DY3747
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY3747
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Canine IL-1 beta / IL-1F2 ELISA Standard Curve
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Canine IL-1 beta/IL-1F2 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
7.8 - 500 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Canine IL-1 beta / IL-1F2 ELISA Standard Curve View Larger

Canine IL-1 beta / IL-1F2 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.

Long Name:
Interleukin 1 beta
Entrez Gene IDs:
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names:
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Citations for Canine IL-1 beta/IL-1F2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. MicroRNA-194 regulates parasitic load and IL-1?-dependent nitric oxide production in the peripheral blood mononuclear cells of dogs with leishmaniasis
    Authors: Costa, SF;Soares, MF;Poleto Bragato, J;Dos Santos, MO;Rebech, GT;de Freitas, JH;de Lima, VMF;
    PLoS neglected tropical diseases
    Species: Canine
    Sample Types: Cell Culture Supernates
  2. miR-148a regulation interferes in inflammatory cytokine and parasitic load in canine leishmaniasis
    Authors: GT Rebech, JP Bragato, SF Costa, JH de Freitas, MO Dos Santos, MF Soares, FR Eugênio, PSP Dos Santos, VMF de Lima
    PloS Neglected Tropical Diseases, 2023-01-31;17(1):e0011039.
    Species: Canine
    Sample Types: Cell Culture Supernates
  3. Mycobacterium intracellulare induces a Th17 immune response via M1-like macrophage polarization in canine peripheral blood mononuclear cells
    Authors: S Kim, YS Hyun, HT Park, MK Shin, HS Yoo
    Scientific Reports, 2022-07-12;12(1):11818.
    Species: Canine
    Sample Types: Cell Culture Supernates
  4. Effect of a single intra-articular administration of stanozolol in a naturally occurring canine osteoarthritis model: a randomised trial
    Authors: JC Alves, A Santos, P Jorge, C Lavrador, LM Carreira
    Scientific Reports, 2022-04-07;12(1):5887.
    Species: Canine
    Sample Types: Synovial Fluid
  5. Evolutionary loss of inflammasomes in the Carnivora and implications for the carriage of zoonotic infections
    Authors: Z Digby, P Tourlomous, J Rooney, JP Boyle, B Bibo-Verdu, RJ Pickering, SJ Webster, TP Monie, LJ Hopkins, N Kayagaki, GS Salvesen, S Warming, L Weinert, CE Bryant
    Cell Reports, 2021-08-24;36(8):109614.
    Species: Canine
    Sample Types: Cell Culture Supernates
  6. The intra-articular administration of triamcinolone hexacetonide in the treatment of osteoarthritis. Its effects in a naturally occurring canine osteoarthritis model
    Authors: JC Alves, A Santos, P Jorge, C Lavrador, LM Carreira
    PLoS ONE, 2021-01-20;16(1):e0245553.
    Species: Canine
    Sample Types: Serum
  7. Early activation of deleterious molecular pathways in the kidney in experimental heart failure with atrial remodeling
    Authors: T Ichiki, BK Huntley, GJ Harty, SJ Sangaralin, JC Burnett
    Physiol Rep, 2017-05-15;5(9):.
    Species: Canine
    Sample Types: Tissue Homogenates

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