Canine IL-1 beta /IL-1F2 Biotinylated Antibody

Catalog #: BAF3747 Datasheet / COA / SDS

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BAF3747

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Canine IL-1 beta /IL-1F2 Biotinylated Antibody Summary

Species Reactivity

Canine

Specificity

Detects canine IL‑1 beta /IL‑1F2 in Western blots. In Western blots, approximately 40% cross-reactivity with recombinant feline IL-1 beta is observed, 10% cross-reactivity with recombinant human IL-1 beta, recombinant rhesus macaque IL-1 beta, recombinant equine IL-1 beta, and recombinant porcine IL-1 beta is observed, and 5% cross-reactivity with recombinant mouse IL-1 beta, recombinant rat IL-1 beta, and recombinant cotton rat IL-1 beta is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant canine IL‑1 beta /IL‑1F2
Asp114-Ser265
Accession # Q28292

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Cotton Rat IL‑1 beta /IL‑1F2 (Catalog # 1009-CL)

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

Canine peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 22% amino acid (aa) identity in dog. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The canine IL-1 beta cDNA encodes a 266 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature canine IL-1 beta shares 68-78% aa sequence identity with cotton rat, equine, feline, human, mouse, porcine, rat, and rhesus macaque IL-1 beta.

References

Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
Accession # NP_001033060.
Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Long Name

Interleukin 1 beta

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Alternate Names

catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta