DC-Cholesterol hydrochloride

Tocris Bioscience | Catalog # 7176

Cationic cholesterol derivative
Tocris Bioscience
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Key Product Details

Description

Cationic cholesterol derivative

Product Description

DC-Cholesterol hydrochloride is a cationic cholesterol derivative for use as a liposomal transfection reagent. Combines with the 'helper lipid' DOPE (Cat. No. 7175) after sonication or microfluidization to form unilamellar liposomes. Can be used in vitro and in vivo for transfer of nucleic acids, protein complexes, or other small molecule complexes into tissues, tumors and cells.

Product Specifications for DC-Cholesterol hydrochloride

Molecular Weight

537.27

Formula

C32H56N2O2.HCl

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

(3β)-3-[N-[2-(Dimethylamino)ethyl]carbamate cholest-5-en-3-ol hydrochloride

CAS Number

166023-21-8

PubChem ID

16219102

InChI Key

ISXSJGHXHUZXNF-LXZPIJOJSA-N

SMILES

[H][C@]1([C@@H](CCCC(C)C)C)CC[C@]2([C@@]3(CC=C4C[C@@H](OC(NCCN(C)C)=O)CC[C@@]4([C@]3(CC[C@]12C)[H])C)[H])[H].Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 2.69 5 with sonication
Ethanol 10.75 20

Preparing Stock Solutions for DC-Cholesterol hydrochloride

The following data is based on the product molecular weight 537.27.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 9.31 mL 46.53 mL 93.06 mL
1 mM 1.86 mL 9.31 mL 18.61 mL
2 mM 0.93 mL 4.65 mL 9.31 mL
10 mM 0.19 mL 0.93 mL 1.86 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

  • Li DC-Chol lipid system in gene transfer. J.Control.Release

Product Documents for DC-Cholesterol hydrochloride

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DC-Cholesterol hydrochloride

For research use only

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