Equine IL-1 beta /IL-1F2 Antibody

Catalog #: MAB3340 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3340
MAB3340-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Equine IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity

Equine

Specificity

Detects equine IL‑1 beta /IL‑1F2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant canine IL-1 beta, recombinant cotton rat IL-1 beta, recombinant feline IL-1 beta, recombinant human (rh) IL‑1 beta, recombinant porcine IL-1 beta, recombinant rat IL‑1 beta, rhIL-1H1, or recombinant mouse IL-17R beta is observed.

Source

Monoclonal Rat IgG_2A Clone # 424823

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant equine IL‑1 beta /IL‑1F2
Ala116-Ala268 (Glu179Gly, Met188Thr, Thr194Ile, Ser245Lys, Arg256Gln)
Accession # NP_001075995

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Equine IL‑1 beta /IL‑1F2 (Catalog # 3340-EL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in equine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The equine IL-1 beta cDNA encodes a 268 aa precursor. A 115 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). An alternatively spliced form of equine IL-1 beta has a deletion which encompasses the Caspase-1 cleavage site and potentially results in a membrane-associated form (8). The 17 kDa mature equine IL-1 beta shares 65%-75% aa sequence identity with canine, cotton rat, feline, human, mouse, porcine, rat, and rhesus IL-1 beta.

References

Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
Kato, H. et al. (1997) Vet. Immunol. Immunopathol. 48:221.
Howard, R.D. et al. (1998) Am. J. Vet. Res. 59:704.
Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Kato, H. et al. (1996) Gene 177:11.

Long Name

Interleukin 1 beta

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Alternate Names

catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta