Equine IL-1 beta /IL-1F2 Antibody

Catalog #: AF3340 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3340
AF3340-SP

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Equine IL‑1 beta /IL‑1F2 Antibody.

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Equine IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity

Equine

Specificity

Detects equine IL‑1 beta /IL‑1F2 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant equine IL‑1 beta /IL‑1F2
Ala116-Ala268 (Glu179Gly, Met188Thr, Thr194Ile, Ser245Lys, Arg256Gln)
Accession # Q28386

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Equine IL‑1 beta /IL‑1F2 (Catalog # 3340-EL)

Immunocytochemistry

20-35 µg/mL

See below

Neutralization

Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 0.05-0.25 µg/mL in the presence of 100 pg/mL Recombinant Equine IL‑1 beta /IL‑1F2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Equine IL‑1 beta /IL‑1F2 Antibody. Recombinant Equine IL-1 beta / IL-1F2 (Catalog # 3340-EL) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Equine IL-1 beta /IL-1F2 (100 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine IL-1 beta /IL-1F2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF3340). The ND₅₀ is typically 0.05-0.25 µg/mL.

Immunocytochemistry

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IL‑1 beta /IL‑1F2 in Equine PBMCs. IL-1 beta /IL-1F2 was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Equine IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3340) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in equine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The equine IL-1 beta cDNA encodes a 268 aa precursor. A 115 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). An alternatively spliced form of equine IL-1 beta has a deletion which encompasses the Caspase-1 cleavage site and potentially results in a membrane-associated form (8). The 17 kDa mature equine IL-1 beta shares 65%-75% aa sequence identity with canine, cotton rat, feline, human, mouse, porcine, rat, and rhesus IL-1 beta.

References

Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
Kato, H. et al. (1997) Vet. Immunol. Immunopathol. 48:221.
Howard, R.D. et al. (1998) Am. J. Vet. Res. 59:704.
Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Kato, H. et al. (1996) Gene 177:11.

Long Name

Interleukin 1 beta

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Alternate Names

catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta