ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free

Catalog #: CCM037 Datasheet / COA / SDS

For potent and highly proliferative NK Cells

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CCM037

ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free

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ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free Summary

Animal Component-Free Media for the ex vivo expansion and culture of human Natural Killer lymphocytes.

Key Benefits

• Supports feeder- and serum-free expansion of NK cells
• Phenol red-free formulation
• Animal component-free media to support patient safety
• Supports NK cell expansion from PBMCs or purified CD3⁺-depleted PBMCs
• Protocol to support feeder-cell G-Rex culture process
• Protocol to support feeder-free G-Rex culture process

Why Use ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free?

Natural Killer (NK) cells play an important role in both the adaptive and innate immune responses that control infection, autoimmunity, and tumor immunosurveillance. Due to their intrinsic and non-specific anti-tumor activity, human NK cells have been employed as anti-cancer therapies. Robust expansion and maintenance of NK Cells is critical to facilitate NK cell therapy.

Versatility of ExCellerate Human NK Cell Expansion Media

ExCellerate Human NK Cell Expansion Media is optimized for NK cell expansion when used in combination with Recombinant Human Cytokines from R&D Systems; however, this versatile media also provides robust growth using a variety of cytokine combinations (IL-2, IL-15, IL-12, IL-18, and IL-21) and cell activation methods, including particle, bead-based, or plate-bound Human NKp46/NCR1 Antibody. The media supports both feeder-based and feeder-free expansion methods.

Kit Contents

1 L of ExCellerate Human NK Cell Expansion Media, Animal Component-Free

Specifications

Shipping Conditions

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the recommended temperature. Protect from light.

Storage

Store the unopened product at -20 °C. Do not use past expiration date. Protect from Light.

Species

Human

Country of Origin

UNITED STATES

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

Product Datasheet

COA

Scientific Data

Data

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Only ExCellerate CCM037 supports both feeder-free and serum-free expansion of NK cells. Purified NK cells were plated at 2.5e⁶/cm² in a 24-well G-Rex using media without serum supplementation. NK cells were stimulated with 250 IU/mL IL-2, 2 ng/mL IL-12 (at initial seeding only), and 10 ng/mL each of IL-15, IL-18, and IL-21. After 7 days, NK cells were reseeded at a density of 0.05e⁶/cm² and grown for a total of 14 days. Data labels are cell viability at harvest.

Data

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ExCellerate CCM037 supports feeder-free expansion of NK cells better than competitor medias. Purified NK cells were plated at 2.5e⁶/cm² in a 24-well G-Rex using medias supplemented with 5% hAB serum. NK cells were stimulated with 250 IU/mL IL-2, 2 ng/mL IL-12 (at initial seeding only), and 10 ng/mL each of IL-15, IL-18, and IL-21. After 7 days, NK cells were reseeded at a density of 0.05e⁶/cm² and grown for a total of 14 days. Data labels are cell viability at harvest.

Data

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NK cells grown in ExCellerate CCM037 with or without serum show similar phenotypes. Day 14 expression profiles of NK cells grown in feeder-free, cytokine stimulated culture conditions either with or without serum supplementation, 3 donor average.

Data

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NK cells grown under feeder-free conditions in serum-containing medias maintain cytotoxic activity. Day 14 cytokine-expanded NK cells were challenged with K562 target cells in a 4 hour luciferase kill assay. NK cells grown in CCM037 show cytotoxic activity comparable to competitor medias.

Data

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ExCellerate CCM037 supports serum free expansion of gene edited NK cells. Purified NK cells were seeded in a G-Rex culture vessel using CCM037 without serum supplementation and stimulated with K562 feeder cells. Gene editing was performed on day 4 using TcBuster transposase to insert a CD19-CAR. NK cells were restimulated at day 7 and 14 with K562 feeder cells. Data labels are cell viability at harvest.