Home / NKp46/NCR1 / Human NKp46/NCR1 Antibody

Human NKp46/NCR1 Antibody

Catalog #: MAB1850
46 Citations 6 Reviews Datasheet / COA / SDS
Clone 195314 was used by HLDA to establish CD designation
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MAB1850-SP
MAB1850-100
MAB1850-500
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NKp46/NCR1 in NK-92 Human Cell Line and Human PBMCs.
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Citations (46)
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Reviews (6)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NKp46/NCR1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NKp46/NCR1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 195314
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse T cell hybridoma transfected with human NKp46/NCR1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human NKp46/NCR1 Fc Chimera (Catalog # 1850-NK)
Flow Cytometry
0.25 µg/106 cells
Human whole blood CD56+ natural killer cells
Immunohistochemistry
5-25 µg/mL
See below
Agonist Activity
Measured by its ability to induce IFN-gamma secretion by NK-92 human natural killer lymphoma cells.
The ED50 for this effect is typically ≤ 1 μg/mL.
 
CyTOF-reported
Horowitz, A. et al. Sci. Transl. Med. (2013) 208ra145. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Dual RNAscope ISH-IHC
3-25 µg/mL
Immersion fixed paraffin-embedded sections of human tonsil
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry NKp46/NCR1 antibody in NK-92 Human Cell Line and Human PBMCs by Immunocytochemistry (ICC). View Larger

NKp46/NCR1 in NK-92 Human Cell Line and Human PBMCs. NKp46/NCR1 was detected in immersion fixed NK-92 human natural killer lymphoma cell line (left panel, positive stain) and human peripheral blood mononuclear cells (PBMCs; right panel, negative stain) using Mouse Anti-Human NKp46/NCR1 Monoclonal Antibody (Catalog # MAB1850) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry NKp46/NCR1 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

NKp46/NCR1 in Human Tonsil. NKp46/NCR1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human NKp46/NCR1 Monoclonal Antibody (Catalog # MAB1850) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to natural killer cells in germinal center. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Flow Cytometry View Larger

Detection of NKp46/NCR1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human NKp46/NCR1 Monoclonal Antibody (Catalog # MAB1850) or (B) Mouse IgG2B Isotype Control (MAB0041) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Rabbit Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (FAB24086P). Staining was performed using our Staining Membrane-associated Proteins protocol.

In-situ Hybridization View Larger

Detection of NKp46/NCR1 in Human Tonsil. Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for NKp46 mRNA (ACD RNAScope Probe, catalog #312658; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human NKp46 monoclonal antibody (R&D Systems catalog # MAB1850) at 15ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cell surface.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKp46/NCR1

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. The CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is up-regulated by IL-2. NKp46 is a type I transmembrane protein with two extracellular Ig-like domains followed by a short stalk region, a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. Through its positive charge in the transmembrane domain, NKp46 associates with the ITAM‑bearing signal adapter proteins, CD3 zeta and Fc epsilon R1 gamma, which are able to form disulfide-linked homodimers and heterodimers (3, 8). Studies with neutralizing antibodies indicate that the three NCRs are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (4). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (5‑7). Human NKp46 shares 58% and 59% amino acid sequence identity with the mouse and rat proteins, respectively.

References
  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255. 
  2. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235. 
  4. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  5. Arnon, T. et al. (2004) Blood 103:664.
  6. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
  7. Mandelboim, O. et al. (2001) Nature 409:1055.
  8. Moretta, A. et al. (2001) Annu. Rev. Immunol. 19:197.
Entrez Gene IDs
9437 (Human); 17086 (Mouse); 100141419 (Porcine); 102115479 (Cynomolgus Monkey)
Alternate Names
CD335 antigen; CD335; hNKp46; Ly94; LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); Lymphocyte antigen 94 homolog; MAR-1; natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; NCR1; NK cell-activating receptor; NKp46; NKP46FLJ99094; NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)

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Citations for Human NKp46/NCR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

46 Citations: Showing 1 - 10
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  1. Expression of human Interferon Regulatory Factor 3 (IRF-3) in alveolar macrophages relates to clinical and functional traits in COPD
    Authors: Baraldo, S;Bonato, M;Cassia, S;Casolari, P;De Ferrari, L;Tiné, M;Baraldi, F;Bigoni, T;Riccio, AM;Braido, F;Saetta, M;Papi, A;Contoli, M;
    Respiratory research
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Characterization of HIF-1? Knockout Primary Human Natural Killer Cells Including Populations in Allogeneic Glioblastoma
    Authors: Nakazawa, T;Morimoto, T;Maeoka, R;Yamada, K;Matsuda, R;Nakamura, M;Nishimura, F;Yamada, S;Park, YS;Tsujimura, T;Nakagawa, I;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. An efficient feeder-free and chemically-defined expansion strategy for highly purified natural killer cells derived from human cord blood
    Authors: Nakazawa, T;Maeoka, R;Morimoto, T;Matsuda, R;Nakamura, M;Nishimura, F;Yamada, S;Nakagawa, I;Park, YS;Ito, T;Nakase, H;Tsujimura, T;
    Regenerative therapy
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Capture
  4. Suppression of the antitumoral activity of natural killer cells under indirect coculture with cancer-associated fibroblasts in a pancreatic TIME-on-chip model
    Authors: Kim, HA;Kim, H;Nam, MK;Park, JK;Lee, MY;Chung, S;Lee, KM;Kuh, HJ;
    Cancer cell international
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Sex Differences in the Systemic and Local Immune Response of Pancreatic Cancer Patients
    Authors: A Ahmed, S Köhler, R Klotz, N Giese, T Hackert, C Springfeld, D Jäger, N Halama
    Cancers, 2023-03-17;15(6):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Human Hematopoietic Stem Cell Engrafted IL-15 Transgenic NSG Mice Support Robust NK Cell Responses and Sustained HIV-1 Infection
    Authors: SA Abeynaike, TR Huynh, A Mehmood, T Kim, K Frank, K Gao, C Zalfa, A Gandarilla, L Shultz, S Paust
    Viruses, 2023-01-27;15(2):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Cytotoxic CD8+ T cells may be drivers of tissue destruction in Sj�gren's syndrome
    Authors: N Kaneko, H Chen, CA Perugino, T Maehara, R Munemura, S Yokomizo, J Sameshima, TJ Diefenbach, KR Premo, A Chinju, Y Miyahara, M Sakamoto, M Moriyama, JH Stone, S Nakamura, S Pillai
    Scientific Reports, 2022-09-14;12(1):15427.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. The Combination of Radiotherapy and Complement C3a Inhibition Potentiates Natural Killer cell Functions Against Pancreatic Cancer
    Authors: Quaovi H. Sodji, Dhanya K. Nambiar, Vignesh Viswanathan, Rie von Eyben, Deana Colburg, Michael S. Binkley et al.
    Cancer Research Communications
  9. Balanced engagement of activating and inhibitory receptors mitigates human NK cell exhaustion
    Authors: JA Myers, D Schirm, L Bendzick, R Hopps, C Selleck, P Hinderlie, M Felices, JS Miller
    JCI Insight, 2022-08-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  10. Establishment of an efficient ex�vivo expansion strategy for human natural killer cells stimulated by defined cytokine cocktail and antibodies against natural killer cell activating receptors
    Authors: T Nakazawa, T Morimoto, R Maeoka, R Matsuda, M Nakamura, F Nishimura, S Yamada, I Nakagawa, YS Park, H Nakase, T Tsujimura
    Regenerative Therapy, 2022-07-21;21(0):185-191.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Capture
  11. Radiotherapy orchestrates natural killer cell dependent antitumor immune responses through CXCL8
    Authors: T Walle, JA Kraske, B Liao, B Lenoir, C Timke, E von Bohlen, F Tran, P Griebel, D Albrecht, A Ahmed, M Suarez-Car, A Jiménez-Sá, T Beikert, A Tietz-Dahl, AN Menevse, G Schmidt, M Brom, JHW Pahl, W Antonopoul, M Miller, RL Perez, F Bestvater, NA Giese, P Beckhove, P Rosenstiel, D Jäger, O Strobel, D Pe'er, N Halama, J Debus, A Cerwenka, PE Huber
    Science Advances, 2022-03-23;8(12):eabh4050.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Flow cytometric single cell-based assay to simultaneously detect cell death, cell cycling, DNA content and cell senescence
    Authors: E Lieschke, Z Wang, C Chang, CE Weeden, GL Kelly, A Strasser
    Cell Death and Differentiation, 2022-03-09;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. Colorectal Cancer-Associated Immune Exhaustion Involves T and B Lymphocytes and Conventional NK Cells and Correlates With a Shorter Overall Survival
    Authors: Carlo Sorrentino, Luigi D’Antonio, Cristiano Fieni, Stefania Livia Ciummo, Emma Di Carlo
    Frontiers in Immunology
  14. Peripheral blood and tissue assessment highlights differential tumor-circulatory gradients of IL2 and MIF with prognostic significance in resectable pancreatic ductal adenocarcinoma
    Authors: Azaz Ahmed, Sophia Köhler, Rosa Klotz, Nathalia Giese, Felix Lasitschka, Thilo Hackert et al.
    OncoImmunology
  15. Association Between SARS-CoV-2 Infection and Immune-Mediated Myopathy in Patients Who Have Died
    Authors: Tom Aschman, Julia Schneider, Selina Greuel, Jenny Meinhardt, Simon Streit, Hans-Hilmar Goebel et al.
    JAMA Neurology
  16. Natural killer cells and cytotoxic T lymphocytes are required to clear solid tumor in a patient-derived xenograft
    Authors: Duy Tri Le, Tridu R. Huynh, Bryan Burt, George Van Buren, Shawn A. Abeynaike, Cristina Zalfa et al.
    JCI Insight
  17. Wilms' Tumor Primary Cells Display Potent Immunoregulatory Properties on NK Cells and Macrophages
    Authors: PF Fiore, P Vacca, N Tumino, F Besi, A Pelosi, E Munari, M Marconi, I Caruana, V Pistoia, L Moretta, B Azzarone
    Cancers, 2021-01-09;13(2):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  18. Neuroblast senescence in the aged brain augments natural killer cell cytotoxicity leading to impaired neurogenesis and cognition
    Authors: WN Jin, K Shi, W He, JH Sun, L Van Kaer, FD Shi, Q Liu
    Nature Neuroscience, 2020-11-30;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Cellular and gene signatures of tumor-infiltrating dendritic cells and natural-killer cells predict prognosis of neuroblastoma
    Authors: O Melaiu, M Chierici, V Lucarini, G Jurman, LA Conti, R De Vito, R Boldrini, L Cifaldi, A Castellano, C Furlanello, V Barnaba, F Locatelli, D Fruci
    Nat Commun, 2020-11-25;11(1):5992.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors
    Authors: JW Choi, S Lim, JH Kang, SH Hwang, KC Hwang, SW Kim, S Lee
    Molecules, 2020-11-09;25(21):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Trastuzumab upregulates programmed death ligand-1 expression through interaction with NK cells in gastric cancer
    Authors: K Yamashita, M Iwatsuki, N Yasuda-Yos, T Morinaga, Y Nakao, K Harada, K Eto, J Kurashige, Y Hiyoshi, T Ishimoto, Y Nagai, S Iwagami, Y Baba, Y Miyamoto, N Yoshida, JA Ajani, H Baba
    Br J Cancer, 2020-10-26;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  22. Chronic Inflammation Permanently Reshapes Tissue-Resident Immunity in Celiac Disease
    Authors: Toufic Mayassi, Kristin Ladell, Herman Gudjonson, James E. McLaren, Dustin G. Shaw, Mai T. Tran et al.
    Cell
  23. Mutated Von Hippel-Lindau-renal cell carcinoma (RCC) promotes patients specific natural killer (NK) cytotoxicity
    Authors: AM Trotta, S Santagata, S Zanotta, C D'Alterio, M Napolitano, G Rea, R Camerlingo, F Esposito, E Lamantia, A Anniciello, G Botti, N Longo, G Botti, S Pignata, S Perdonà, S Scala
    J. Exp. Clin. Cancer Res., 2018-12-04;37(1):297.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  24. Targeted therapies for targeted populations: Anti-EGFR treatment for EGFR amplified gastroesophageal adenocarcinoma
    Authors: SB Maron, L Alpert, HA Kwak, S Lomnicki, L Chase, D Xu, E O'Day, RJ Nagy, RB Lanman, F Cecchi, T Hembrough, A Schrock, J Hart, SY Xiao, N Setia, DVT Catenacci
    Cancer Discov, 2018-02-15;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  25. Infiltration and persistence of lymphocytes during late-stage cerebral ischemia in middle cerebral artery occlusion and photothrombotic stroke models
    Authors: Y Feng, S Liao, C Wei, D Jia, K Wood, Q Liu, X Wang, FD Shi, WN Jin
    J Neuroinflammation, 2017-12-15;14(1):248.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  26. Low-risk population among patients with tumor-node-metastasis stage III/IV oral squamous cell carcinoma
    Authors: Tohru Ikeda, Sachiko Seki, Mutsunori Fujiwara, Masaaki Matsuura, Yuu Ozaki-Honda, Shuichi Fujita et al.
    Oncology Letters
  27. Activated hepatic stellate cells impair NK cell anti-fibrosis capacity through a TGF-?-dependent emperipolesis in HBV cirrhotic patients
    Authors: J Shi, J Zhao, X Zhang, Y Cheng, J Hu, Y Li, X Zhao, Q Shang, Y Sun, B Tu, L Shi, B Gao, FS Wang, Z Zhang
    Sci Rep, 2017-03-14;7(0):44544.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  28. Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome
    J Immunol, 2016-08-10;197(5):1621-30.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  29. Natural Killer-like B Cells Prime Innate Lymphocytes against Microbial Infection.
    Authors: Wang S, Xia P, Chen Y, Huang G, Xiong Z, Liu J, Li C, Ye B, Du Y, Fan Z
    Immunity, 2016-07-12;45(1):131-44.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. NKp30 isoforms and NKp46 transcripts in metastatic melanoma patients: Unique NKp30 pattern in rare melanoma patients with favorable evolution
    Authors: M Messaouden, G Fregni, D Enot, N Jacquelot, E Neves, N Germaud, HJ Garchon, W Boukouaci, R Tamouza, J Chanal, MF Avril, A Toubert, L Zitvogel, S Rusakiewic, A Caignard
    Oncoimmunology, 2016-03-10;5(12):e1154251.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  31. Human liver-resident CD56(bright)/CD16(neg) NK cells are retained within hepatic sinusoids via the engagement of CCR5 and CXCR6 pathways.
    Authors: Hudspeth K, Donadon M, Cimino M, Pontarini E, Tentorio P, Preti M, Hong M, Bertoletti A, Bicciato S, Invernizzi P, Lugli E, Torzilli G, Gershwin M, Mavilio D
    J Autoimmun, 2015-08-30;66(0):40-50.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  32. Characterization of the Microenvironment in Positive and Negative Sentinel Lymph Nodes from Melanoma Patients.
    Authors: Messaoudene M, Perier A, Fregni G, Neves E, Zitvogel L, Cremer I, Chanal J, Sastre-Garau X, Deschamps L, Marinho E, Larousserie F, Maubec E, Avril M, Caignard A
    PLoS ONE, 2015-07-28;10(7):e0133363.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  33. A novel tetravalent bispecific TandAb (CD30/CD16A) efficiently recruits NK cells for the lysis of CD30+ tumor cells.
    Authors: Reusch U, Burkhardt C, Fucek I, Le Gall F, Le Gall M, Hoffmann K, Knackmuss S, Kiprijanov S, Little M, Zhukovsky E
    MAbs, 2014-03-26;6(3):728-39.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  34. CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph.
    Authors: Carrega, Paolo, Bonaccorsi, Irene, Di Carlo, Emma, Morandi, Barbara, Paul, Petra, Rizzello, Valeria, Cipollone, Giuseppe, Navarra, Giuseppe, Mingari, Maria Cr, Moretta, Lorenzo, Ferlazzo, Guido
    J Immunol, 2014-03-19;192(8):3805-15.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  35. Mature cytotoxic CD56(bright)/CD16(+) natural killer cells can infiltrate lymph nodes adjacent to metastatic melanoma.
    Cancer Res, 2013-11-13;74(0):81.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  36. Natural killer cells are characterized by the concomitantly increased interferon-gamma and cytotoxicity in acute resolved hepatitis B patients.
    Authors: Zhao J, Li Y, Jin L, Zhang S, Fan R, Sun Y, Zhou C, Shang Q, Li W, Zhang Z, Wang F
    PLoS ONE, 2012-11-01;7(11):e49135.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  37. Sequential metastases of colorectal cancer: Immunophenotypes and spatial distributions of infiltrating immune cells in relation to time and treatments.
    Authors: Keim S, Zoernig I, Spille A, Lahrmann B, Brand K, Herpel E, Grabe N, Jager D, Halama N
    Oncoimmunology, 2012-08-01;1(5):593-599.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  38. Dimerization of NKp46 Receptor Is Essential for NKp46-Mediated Lysis: Characterization of the Dimerization Site by Epitope Mapping
    Authors: Michal Jaron-Mendelson, Rami Yossef, Michael Y. Appel, Alon Zilka, Uzi Hadad, Fabian Afergan et al.
    The Journal of Immunology
  39. Innate immunity in multiple sclerosis white matter lesions: expression of natural cytotoxicity triggering receptor 1 (NCR1).
    Authors: Durrenberger PF, Ettorre A, Kamel F
    J Neuroinflammation, 2012-01-02;9(0):1.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  40. NKp46 identifies an NKT cell subset susceptible to leukemic transformation in mouse and human.
    Authors: Yu J, Mitsui T, Wei M, Mao H, Butchar JP, Shah MV, Zhang J, Mishra A, Alvarez-Breckenridge C, Liu X, Liu S, Yokohama A, Trotta R, Marcucci G, Benson DM, Loughran TP, Tridandapani S, Caligiuri MA
    J. Clin. Invest., 2011-04-01;121(4):1456-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  41. An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation.
    Authors: Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socie G, Toubert A
    J. Immunol., 2008-08-01;181(3):2227-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  42. Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
    Authors: Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD, Barlogie B, van Rhee F
    Blood, 2007-10-18;111(3):1309-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  43. A novel binding assay to assess specificity of monoclonal antibodies.
    Authors: Warren HS, Warren</LastName><ForeNam HS</Initia, Bettadapura J
    J. Immunol. Methods, 2005-08-15;305(1):33-8.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Epitope Mapping
  44. Case Report: A Peculiar Case of Inflammatory Colitis After SARS-CoV-2 Infection
    Authors: Rutigliani M, Bozzo M, Barberis A et al.
    Frontiers in immunology
  45. An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer
    Authors: Johnson BE, Creason AL, Stommel JM et al.
    Cell reports. Medicine
  46. Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer
    Authors: Jung-Won Choi, Eui Soo Lee, Se Young Kim, Su Il Park, Sena Oh, Jung Hwa Kang et al.
    BMC Cancer

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Human NKp46/NCR1 Antibody
By Anonymous on 08/09/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Natural killer cells Species: Human

NKp46/NCR1 in NK-92 human natural killer lymphoma cell line

Immunocytochemistry/Immunofluorescence Human NKp46/NCR1 Antibody MAB1850
Human NKp46/NCR1 Antibody
By Anonymous on 05/18/2018
Application: IHC Sample Tested: Lymph node tissue Species: Human

Paraffin embedded lymph node metastasis samples
Antigen retrieval: Citrat buffer
Antibody Dilution: 1:50
Buffer: TBS-T

At this concentration, the isotype showed no specific staining, whereas MAB1850 showed clear surface staining of cells.

Immunohistochemistry Human NKp46/NCR1 Antibody MAB1850
Human NKp46/NCR1 Antibody
By Becker Law on 10/01/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Kidney (cortex) tissue Species: Human
Immunocytochemistry/Immunofluorescence Human NKp46/NCR1 Antibody MAB1850
Human NKp46/NCR1 Antibody
By Anonymous on 10/26/2015
Application: IHC Sample Tested: Colon tissue Species: Human

At this concentration there is little backgroud.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1:500

Immunohistochemistry Human NKp46/NCR1 Antibody MAB1850
Human NKp46/NCR1 Antibody
By Anonymous on 10/26/2015
Application: IHC Sample Tested: Colon tissue Species: Human

Seems like the immune cells show a little staining. At 1:250 all cells showed staining. Dilutions are important.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1:1000

Immunohistochemistry Human NKp46/NCR1 Antibody MAB1850
Human NKp46/NCR1 Antibody
By Anonymous on 10/26/2015
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Natural killer cells Species: Human

Human NKp46/NCR1 MAb (Clone 195314), works well for detection of NKp46 on surface of NK cells.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1:500

Immunocytochemistry/Immunofluorescence Human NKp46/NCR1 Antibody MAB1850