ExCellerate™ T Cell Media, Animal Component-Free

Catalog #: CCM035 Datasheet / COA / SDS
Newer Version Available: CCM038-GMP-1L
NEW
This media has been discontinued. Please see CCM038-GMP for cell therapy applications and CCM030 for basic T cell research applications

Discontinued Product

CCM035 has been discontinued and is replaced by CCM038-GMP-1L.

ExCellerate™ Human T Cell Media, Animal-Component Free
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ExCellerate™ T Cell Media, Animal Component-Free Summary

This media has been discontinued. Please see CCM038 for cell therapy applications and see CCM030 for basic T cell research applications.
 

Key Benefits

• Phenol red-free formulation in 1 L bottles
• Robust T cell expansion under serum-free and animal component-free conditions
• Optimized for use with Bio-Techne cytokines and G-Rex® bioreactors
• Compatible with a variety of T cell activation methods
• Supports T cell expansion from PBMCs or purified CD3+ T cells

ExCellerate T Cell Media is formulated and optimized for the ex vivo culture of human T lymphocytes. Unlike traditional serum-containing culture media, animal component-free media provides a stable culture and optimized environment that facilitates the generation and expansion of T cells. The medium supports expansion of routine culture of human T cells while maintaining high viability and expansion of stem cell memory T Cells.

Versatility of ExCellerate T Cell Media, Animal Component-Free

ExCellerate T Cell Media is optimized for T cell expansion in a G-Rex bioreactor. This media is compatible with a variety of cytokine combinations (IL-2, IL-7, and IL-15) AF RUO Proteins from R&D Systems in different cell activation methods, including particle, bead-based, or plate-bound. The activation reagents, culture vessel, and cytokine growth factor combinations used with this media should be optimized by application or experimental protocol.

Kit Contents

1 L Bottle of ExCellerate T Cell Media, Animal Component-Free

Specifications

Stability
Product is stable for 12 months from date of manufacture when stored at 2-8 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped at 2-8 °C. Upon receipt, store immediately at the recommended temerature. Protect from light.
Storage
Store the unopened product at 2-8 °C protected from light. Do not use past expiration date.
Species
Human

Product Datasheets

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Scientific Data

Cell Culture View Larger

Stem Cell Memory Population is enhanced when using ExCellerate™ Human T Cell Media, ACF compared to other media suppliers. Two donors were evaluated after nine days in culture within a G-Rex® Bioreactor with media supplemented with 10 ng/mL of BT007-AFL and 10 ng/mL of BT-015-AFL. Flow Cytometry was used to evaluate CCR7+ T Cells.

Cell Proliferation View Larger

A fully Animal-Free and Serum-Free T Cell process expands viable T cells similar to other Xeno-Free media alternatives. Two donors were evaluated for expansion and viability during a 9 day culture period in a G-Rex® bioreactor supplemented with 10 ng/mL of BT-007-AFL and 10 ng/mL of BT-015-AFL.

Cell Proliferation View Larger

Excellerate™ Human T Cell Media, Animal-Component Free supports edited and non-edited T cell cultures. Both Lentiviral transduction and TcBuster™ transposition of a CD19 CAR was evaluated at day 8 and 9 of culture within a G-Rex® bioreactor. Both Lentiviral transduction and TcBuster™ transposition of a CD19 CAR was evaluated at day 8 and 9 of culture within a G-Rex® bioreactor.

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