ExCellerate™ T Cell Media, Animal Component-Free
ExCellerate™ T Cell Media, Animal Component-Free Summary
This media has been discontinued. Please see CCM038 for cell therapy applications and see CCM030 for basic T cell research applications.Key Benefits
• Phenol red-free formulation in 1 L bottles
• Robust T cell expansion under serum-free and animal component-free conditions
• Optimized for use with Bio-Techne cytokines and G-Rex® bioreactors
• Compatible with a variety of T cell activation methods
• Supports T cell expansion from PBMCs or purified CD3+ T cells
ExCellerate T Cell Media is formulated and optimized for the ex vivo culture of human T lymphocytes. Unlike traditional serum-containing culture media, animal component-free media provides a stable culture and optimized environment that facilitates the generation and expansion of T cells. The medium supports expansion of routine culture of human T cells while maintaining high viability and expansion of stem cell memory T Cells.
Versatility of ExCellerate T Cell Media, Animal Component-Free
ExCellerate T Cell Media is optimized for T cell expansion in a G-Rex bioreactor. This media is compatible with a variety of cytokine combinations (IL-2, IL-7, and IL-15) AF RUO Proteins from R&D Systems in different cell activation methods, including particle, bead-based, or plate-bound. The activation reagents, culture vessel, and cytokine growth factor combinations used with this media should be optimized by application or experimental protocol.
Kit Contents
1 L Bottle of ExCellerate T Cell Media, Animal Component-Free
Specifications
Product Datasheets
Scientific Data
Stem Cell Memory Population is enhanced when using ExCellerate™ Human T Cell Media, ACF compared to other media suppliers. Two donors were evaluated after nine days in culture within a G-Rex® Bioreactor with media supplemented with 10 ng/mL of BT007-AFL and 10 ng/mL of BT-015-AFL. Flow Cytometry was used to evaluate CCR7+ T Cells.
A fully Animal-Free and Serum-Free T Cell process expands viable T cells similar to other Xeno-Free media alternatives. Two donors were evaluated for expansion and viability during a 9 day culture period in a G-Rex® bioreactor supplemented with 10 ng/mL of BT-007-AFL and 10 ng/mL of BT-015-AFL.
Excellerate™ Human T Cell Media, Animal-Component Free supports edited and non-edited T cell cultures. Both Lentiviral transduction and TcBuster™ transposition of a CD19 CAR was evaluated at day 8 and 9 of culture within a G-Rex® bioreactor. Both Lentiviral transduction and TcBuster™ transposition of a CD19 CAR was evaluated at day 8 and 9 of culture within a G-Rex® bioreactor.
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