GABA-A R alpha 1 C-Terminus Antibody Summary
Applications
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Scientific Data
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Detection of GABAAR alpha 1 C-Terminus by Western Blot Western blot of forebrain lysates from wildtype and alpha 1 knockout animals, using approximately 5 - 7 μg of tissue per lane. Blots were incubated overnight at 2 - 8° C with anti-GABAAR, alpha 1 subunit, C-Terminus diluted 1:1000. The antibody labelled the ~51kDa alpha 1 subunit of the GABAA R (Control). The labeling was absent from a lysate prepared from alpha 1 knockout animals.
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Background: GABA-A R alpha 1
GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta, and gamma subunits. The rat alpha 1 isoform is a 50 - 52 kDa, 428 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 15 - 222). As with many receptors, phosphorylation is used as a regulatory mechanism. CaM kinase II is known to phosphorylate the alpha 1 subunit and regulate benzodiazepine binding. alpha 1 subunits are particularly abundant in the cerebellum and may contribute to GABA receptor distribution. In the hippocampus and amygdala, the alpha 1 subunit may contribute to amnesia.
- Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
- Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
- Churn, S.B. et al. (2002) J. Neurochem. 82:1065.
- Kralic, J.E. et al. (2006) J. Comp. Neurol. 495:408.
- Sonner, J.M. et al. (2006) Mol. Pharmacol. 68:61.
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Citations for GABA-A R alpha 1 C-Terminus Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors
Authors: Angela L. Whittsette, Ya-Juan Wang, Ting-Wei Mu
iScience
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Grp94 Protein Delivers ?-Aminobutyric Acid Type A (GABAA) Receptors to Hrd1 Protein-mediated Endoplasmic Reticulum-associated Degradation
Authors: XJ Di, YJ Wang, DY Han, YL Fu, AS Duerfeldt, BS Blagg, TW Mu
J. Biol. Chem., 2016-03-04;291(18):9526-39.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot
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