Human A33 Biotinylated Antibody

Name: Human A33 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF3080
Rating: 5 (1 reviews)

Catalog #: BAF3080 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF3080

Detection of A33 in HT‑29 Human Cell Line by Flow Cytometry.

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Human A33 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human A33 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse A33 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human A33
Ile22-Val235
Accession # Q99795

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human A33

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of A33 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon carcinoma cell line was stained with Goat Anti-Human A33 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3080, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: A33

Human A33, also known as GPA33, is a 43 kDa type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.

Long Name

Cell Surface A33 Antigen

Entrez Gene IDs

10223 (Human); 59290 (Mouse)

Alternate Names

A33; cell surface A33 antigen; glycoprotein A33 (transmembrane); Glycoprotein A33; GPA33; MGC129986; MGC129987; transmembrane glycoprotein A33