Human ACLP Antibody

Catalog #: MAB6425 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6425
MAB6425-SP

Detection of ACLP in HASMC Human Cells by Flow Cytometry.

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All ACLP Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ACLP Antibody Summary

Species Reactivity

Human

Specificity

Detects human ACLP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse ACLP is observed.

Source

Monoclonal Mouse IgG₁ Clone # 590831

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ACLP
Glu345-Phe1158
Accession # Q8IUX7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of ACLP antibody in HASMC Human Cells antibody by Flow Cytometry.

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Detection of ACLP in HASMC Human Cells by Flow Cytometry. HASMC human human aortic smooth muscle cells was stained with Human ACLP Monoclonal Antibody (Catalog # MAB6425, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Intracellular Staining by Flow Cytometry

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Detection of ACLP in Molt-4 Human Cells by Flow Cytometry. Molt-4 human human cell line was stained with Human ACLP Monoclonal Antibody (Catalog # MAB6425, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ACLP

Aortic carboxypeptidase-like protein (ACLP), also known as adipocyte enhancer binding protein (AEBP1), is a 175 kDa molecule that contains a discoidin-like domain (aa 383-540) and a peptidase-like domain (aa 560-942). It is expressed in proliferating adipocytes and represses the transcription of FABP4. ACLP promotes macrophage cholesterol retention, the formation of foam cells, and inflammatory responses. It is also an extracellular matrix-associated protein found in fibrotic tissues including vascular smooth muscle. An alternately spliced isoform lacks aa 1-457 and has an altered sequence at its N-terminus. Over aa 345-1158, human ACLP shares 88% aa sequence identity with mouse and rat ACLP.

Long Name

Aortic Carboxypeptidase-like Protein

Entrez Gene IDs

165 (Human)

Alternate Names

ACLP; ACLPadipocyte enhancer binding protein 1; AE binding protein 1; AE-binding protein 1adipocyte enhancer-binding protein 1; AEBP1; Aortic carboxypeptidase-like protein; FLJ33612