Human Akt Pan Specific Antibody Summary
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Accession # P31749
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Akt by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, MCF-7 human breast cancer cell line, and KG-1 human myeloid leukemia cell lines. PVDF membrane was probed with 0.1 µg/mL of Human Akt Pan Specific, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2055) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Akt Pan Specific, at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Akt by Simple WesternTM. Simple Western lane view shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Akt at approximately 60 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human Akt Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2055). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Akt
Rabbit antibodies were raised against a synthetic peptide corresponding to amino acids 75‑90 of human v-akt murine thymoma viral oncogene homolog 1 (Akt1), also known as protein kinase B alpha (PKB alpha ) and RAC-alpha serine/threonine kinase (RAC-PK-alpha ). In addition, this peptide sequence is identical to amino acids 75‑90 of human Akt2 (PKB beta, RAC-PK-beta ), and amino acids 74‑89 of human Akt3 (PKB gamma, RAC-PK-gamma ).
Product Datasheets
Citation for Human Akt Pan Specific Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Method for Recovery and Immunoaffinity Enrichment of Membrane Proteins Illustrated with Metastatic Ovarian Cancer Tissues
Authors: Luke V. Schneider, Varsha Likhte, William H. Wright, Frances Chu, Emma Cambron, Anne Baldwin-Burnett et al.
International Journal of Proteomics
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30 microg cell homogenate/line
Gel SDS/Page 4-12% BisTris Novex Invitrogen.
Western Blot on PVDF membrane.
Blocking Reagent: 5% non fat dry milk/T-TBS
Anti AKT1-2-3 (R& D System cat. N. AF2055)
1:1250 in 3% BSA/PBS o.n. 4°C
Anti pAKTser473 Cell Signalling 1:1250 in 3%
BSA/PBS o.n. 4°C.
Anti horsadish peroxidase linked II antibody against
Rabbit 1:20.000 (GE Healthcare) in T-TBS 90 min r.t.
ECL Prime Amersham (GE Healhtcare).
