Human alpha-Fetoprotein Quantikine ELISA Kit Summary
Product Summary
Recovery
The recovery of AFP spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 99 | 95-103 |
| EDTA Plasma (n=4) | 104 | 94-114 |
| Heparin Plasma (n=4) | 103 | 95-115 |
| Serum (n=4) | 103 | 86-115 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: alpha-Fetoprotein/AFP
alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. It is one of the earliest markers of the hepatocyte lineage. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human alpha-Fetoprotein Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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Tumour-specific activation of a tumour-blood transport improves the diagnostic accuracy of blood tumour markers in mice
Authors: Schmithals, C;Kakoschky, B;Denk, D;von Harten, M;Klug, JH;Hintermann, E;Dropmann, A;Hamza, E;Jacomin, AC;Marquardt, JU;Zeuzem, S;Schirmacher, P;Herrmann, E;Christen, U;Vogl, TJ;Waidmann, O;Dooley, S;Finkelmeier, F;Piiper, A;
EBioMedicine
Species: Mouse
Sample Types: Serum, Cell Culture Supernates
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Effect of Diphtheria Toxin-Based Gene Therapy for Hepatocellular Carcinoma
Authors: K Kamimura, T Yokoo, H Abe, N Sakai, T Nagoya, Y Kobayashi, M Ohtsuka, H Miura, A Sakamaki, H Kamimura, N Miyamura, H Nishina, S Terai
Cancers (Basel), 2020-02-18;12(2):.
Species: Mouse
Sample Types: Serum
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Protein kinase 2 (CK2)�controls CD4+ T cell effector function in the pathogenesis of colitis
Authors: W Yang, SA Gibson, Z Yan, H Wei, J Tao, B Sha, H Qin, EN Benveniste
Mucosal Immunol, 2020-01-27;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Maternal biomarkers for fetal heart failure in fetuses with congenital heart defects or arrhythmias
Authors: T Miyoshi, H Hosoda, M Nakai, K Nishimura, M Miyazato, K Kangawa, T Ikeda, J Yoshimatsu, N Minamino
Am. J. Obstet. Gynecol., 2018-09-28;220(1):104.e1-104.e1.
Species: Human
Sample Types: Serum
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Identification of anti-SF3B1 autoantibody as a diagnostic marker in patients with hepatocellular carcinoma
Authors: HM Hwang, CK Heo, HJ Lee, SS Kwak, WH Lim, JS Yoo, DY Yu, KJ Lim, JY Kim, EW Cho
J Transl Med, 2018-06-28;16(1):177.
Species: Human
Sample Types: Serum
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Serum long noncoding RNA LRB1 as a potential biomarker for predicting the diagnosis and prognosis of human hepatocellular carcinoma
Authors: ZF Wang, R Hu, JM Pang, GZ Zhang, W Yan, ZN Li
Oncol Lett, 2018-05-30;16(2):1593-1601.
Species: Human
Sample Types: Serum
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Knockdown of beta-catenin with dicer-substrate siRNAs reduces liver tumor burden in vivo.
Authors: Dudek H, Wong D, Arvan R, Shah A, Wortham K, Ying B, Diwanji R, Zhou W, Holmes B, Yang H, Cyr W, Zhou Y, Shah A, Farkiwala R, Lee M, Li Y, Rettig G, Collingwood M, Basu S, Behlke M, Brown B
Mol Ther, 2013-10-03;22(1):92-101.
Species: Human
Sample Types: Serum
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DLK1, a serum marker for hepatoblastoma in young infants.
Authors: Falix FA, Aronson DC, Lamers WH, Hiralall JK, Seppen J
Pediatr Blood Cancer, 2011-12-16;59(0):743.
Species: Human
Sample Types: Serum
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Proteomics mapping of cord blood identifies haptoglobin "switch-on" pattern as biomarker of early-onset neonatal sepsis in preterm newborns.
Authors: Buhimschi CS, Bhandari V, Dulay AT, Nayeri UA, Abdel-Razeq SS, Pettker CM, Thung S, Zhao G, Han YW, Bizzarro M, Buhimschi IA
PLoS ONE, 2011-10-10;6(10):e26111.
Species: Human
Sample Types: Serum
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A hypoxia- and alpha-fetoprotein-dependent oncolytic adenovirus exhibits specific killing of hepatocellular carcinomas.
Authors: Kwon OJ, Kim PH, Huyn S, Wu L, Kim M, Yun CO
Clin. Cancer Res., 2010-12-15;16(24):6071-82.
Species: Human
Sample Types: Serum
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Identifying serological biomarkers of hepatocellular carcinoma using surface-enhanced laser desorption/ionization-time-of-flight mass spectroscopy.
Authors: Wu FX, Wang Q, Zhang ZM, Huang S, Yuan WP, Liu JY, Ban KC, Zhao YN
Cancer Lett., 2009-03-05;279(2):163-70.
Species: Human
Sample Types: Serum
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A novel approach and protocol for discovering extremely low-abundance proteins in serum.
Authors: Tanaka Y, Akiyama H, Kuroda T, Jung G, Tanahashi K, Sugaya H, Utsumi J, Kawasaki H, Hirano H
Proteomics, 2006-09-01;6(17):4845-55.
Species: Human
Sample Types: Serum
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