Human Annexin A3 Antibody

Catalog #: MAB4855 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4855
MAB4855-SP

Detection of Human, Mouse, and Rat Annexin A3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Annexin A3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Annexin A3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human Annexin A1, A2, A4, A6, A8, A9, A10, A11, or A13 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 693510

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Annexin A3
Ala2-Asp323
Accession # P12429

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Annexin A3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Annexin A3 Monoclonal Antibody (Catalog # MAB4855) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunohistochemistry

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Annexin A3 in Human Prostate. Annexin A3 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Annexin A3 Monoclonal Antibody (Catalog # MAB4855) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Annexin A3

Annexin A3 (ANXA3), also known as Annexin III and Lipocortin III (LPC3), is a 36 kDa member of the Annexin family of calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexins consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing calcium-dependent phospholipid-binding sites. The C-terminal domain is comprised of four 60-70 amino acid (aa) annexin repeats. Annexin A3 is upregulated in microglia following stroke and in parenchymal hepatocytes in regenerating liver. It can be up- or down‑regulated in various carcinomas. Alternate splicing generates an additional isoform with a 30 amino acid (aa) deletion near the N-terminus. Human Annexin A3 shares 89% and 85% aa sequence identity with mouse and rat Annexin A3, respectively.

Entrez Gene IDs

306 (Human); 11745 (Mouse); 25291 (Rat)

Alternate Names

35-alpha calcimedin; Annexin A3; annexin A3,1,2-cyclic-inositol-phosphate phosphodiesterase; Annexin III; annexin-3; ANXA3; Calcimedin 35-alpha ; calcimedin 35-alpha; Lipocortin III; PAP-III; Placental anticoagulant protein III; placental anticoagulant protein III, calcimedin 35-alpha