Human APC C-Terminus Antibody

Catalog #: AF3695 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3695
AF3695-SP

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Human APC C-Terminus Antibody Summary

Species Reactivity

Human

Specificity

Detects human APC C-Terminus in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human APC
Ser2537-Val2843
Accession # P25054

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human APC C-Terminus

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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APC in Human Colon Cancer Tissue. APC was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using 15 µg/mL Goat Anti-Human APC C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3695) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APC

APC is a ubiquitous, tumor-suppressor protein that is mutated in most colon cancers, generally resulting in a gene product that is truncated in the carboxy terminal region. APC contains several binding domains. The region used as an immunogen overlaps the EB1, DLG (PDZ sequence), and PSD-93 domains. These domains bind microtubules, mitotic spindles, and F-actin and are involved in cell cycle, division, and migration. The immunogen does not include the beta -catenin-binding region, which antagonizes Wnt signaling. The human APC amino acid sequence used as immunogen is 83% and 86% identical to mouse and rat APC, respectively.

Long Name

Adenomatosis Polyposis Coli

Entrez Gene IDs

324 (Human); 11789 (Mouse); 24205 (Rat)

Alternate Names

adenomatosis polyposis coli tumor suppressor; adenomatosis polyposis coli; adenomatous polyposis coli protein; adenomatous polyposis coli; APC; BTPS2; Deleted in polyposis 2.5; DP2; DP2.5DP2; DP3; FPC; GS; Min; Protein APC