Human BASP1 Antibody

Catalog #: AF6479 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6479
AF6479-SP

Detection of Human BASP1 by Western Blot.

1 Image

All BASP1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BASP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human BASP1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BASP1
Gly2-Ala45
Accession # P80723

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human BASP1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and DU145 human prostate carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human BASP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6479) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BASP1 at approximately 60 kDa, possibly a higher molecular weight isoform or a product of SUMOyation. Lower molecular weight isoforms were also observed. This experiment was conducted under reducing conditions on a 4-20% SDS-PAGE gel and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BASP1

BASP1 (Brain acid soluble protein 1; also NAP-22) is a transcriptional cosuppressor that belongs to the BASP1 family of proteins. Although its predicted MW is 23 kDa, BASP1 will show multiple bands in SDS-Page. First, BASP1 forms oligomers in SDS, ranging in size from 30-150 kDa. Second, varying concentrations of SDS will change the apparent BASP1 MW from 56 kDa in 8% SDS-Page to 41 kDa in 13% SDS-Page. At least two forms of BASP1 apparently exist in human, one that is 48-52 kDa, and another that is 32-40 kDa in size. They are not functionally equivalent. BASP1 can be SUMOylated that add 20 kDa in apparent MW. There are also N-terminal fragments termed BIRPs that run from 30-50 kDa in 12% acidic SDS-Page. BASP1 is expressed in neurons, renal podocytes and spermatids. It binds to the cytoplasmic side of the plasmalemma via its myristroylate adduct, and undergoes nuclear translocation when SUMOylated. Human BASP1 is 227 amino acids (aa) in length. It contains a myristoylation site at Gly2, SUMOylation sites at Lys79 and 84, at least eight utilized phosphorylation sites, and an NLS. One splice variant shows a deletion of aa 88-141. Over aa 1-45, human BASP1 shares 96% and 91% aa identity with mouse and rat BASP1, respectively.

Long Name

Brain Acid Soluble Protein 1

Entrez Gene IDs

10409 (Human); 70350 (Mouse); 64160 (Rat)

Alternate Names

BASP1; brain abundant, membrane attached signal protein 1,22 kDa neuronal tissue-enriched acidic protein; brain acid-soluble protein 1; CAP23; CAP-23; CAP23brain acid soluble protein 1; NAP22; NAP-22; NAP22MGC8555; Neuronal axonal membrane protein NAP-22; neuronal tissue-enriched acidic protein