Key Product Details

Species Reactivity

Sheep

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Donkey IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Sheep IgG

Specificity

Sheep IgG specific IgG was purified by sheep IgG (H+L) affinity chromatography and absorbed against human IgG, and human, mouse, bovine, and rabbit serum to eliminate cross-reactivity.

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Scientific Data Images for Sheep IgG (H+L) APC‑conjugated Antibody

Detection of Sheep IgG Primary Antibody by Flow Cytometry

Detection of Sheep IgG Primary Antibody by Flow Cytometry

Human peripheral blood granulocytes were stained with Sheep Anti-Human Fce RIa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6678, filled histogram) or isotype control antibody (Catalog # 5-001-A), open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127) at 10 µL/106cells.

Applications for Sheep IgG (H+L) APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of sheep origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Sheep IgG (H+L) APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Sheep IgG (H+L) APC‑conjugated Antibody

For research use only

Citations for Sheep IgG (H+L) APC‑conjugated Antibody

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FAQs for Sheep IgG (H+L) APC‑conjugated Antibody

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  • Q: Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?

    A: All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.

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