Protocol for the Characterization of Human Th9 Cells

CD4+ T cell subsets exert diverse functions due to their expression of characteristic cytokines. T helper type 9 (Th9) cells constitute one subset of CD4+ effector T cells and are characterized by the secretion of IL-9, IL-10, and IL-21. Th9 cells protect against helminth infection, promote CTL and NK cell mediated anti-tumor immunity, and contribute to chronic inflammation in allergic asthma, ulcerative colitis, psoriasis, autoimmunity, and tuberculosis.

The development of the Th9 subset of CD4+ T cells is induced by distinct extracellular signals (e.g. IL-2, IL-4, IL-6, and TGF-beta) and is controlled by distinct transcription factors (e.g. GATA-3, IRF4, PU.1, STAT6). Th9 polarized cells are present in low abundance in normal human peripheral blood. In vitro differentiation of Th9 cells from the larger CD4+ T cell population provides increased numbers of Th9 cells to facilitate research into their functions.

Products for the Differentiation of Th9 Cells

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T Cell Subsets Poster
CD4+ T Cell Subsets Brochure

Flow Cytometric Analysis of Differentiated Th9 Cells

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Multi-analyte Assay of Cell Supernatants

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Single Analyte Assay of Cell Supernatants

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